FastQCFastQC Report
Mon 26 Jun 2023
SRR315597.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR315597.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10809020
Total Bases432.3 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGG1306101.208342661961954No Hit
ATCTGAGGGTCCAGGGTTCAGGTCCCTGTTCGAAAAAAAA647760.5992772702798218No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGG476790.44110381884759214No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG439140.40627179892349163No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGG350540.32430322082853025No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCAAAAAAAAAAA336730.31152685442343525No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAAAA294970.2728924546351103No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAA286230.2648066152158105No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCAAAACCAAAAA278790.25792347502363766No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATCAAAAAAAAAA225190.20833526073594089No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA206350.19090537347511616No Hit
GGGGGGGGATGCGTGCATTTATCAGATCAAAACCAAAAAA188850.17471519157148382No Hit
GGGGGGGGATGCGTGCATTTATCAGATCAAAAAAAAAAAA183010.16931229658192878No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAAAAAAAAA178970.1655746774453188No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGG168590.15597158669333575No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAAAAA164390.15208594303646397No Hit
CGGTGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAAA160420.14841308462746855No Hit
TCGTCGCGCCGTCGGGTCCCGGGGGAAAAAAAAAAAAAAA156950.14520280284429116No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAA155330.14370405457664062No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA148620.1374962762581622No Hit
ATCTGAGGGTCCAGGGTTCATGTCCCTGTTCGAAAAAAAA142080.13144577399246185No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATAAAAAAAAAAA122960.11375684382117898No Hit
GTCTAGCGGTTAGGATTCCTGAAAAAAAAAAAAAAAAAAA120500.11148096682215408No Hit
GGGGGGGATGCGTGCATTTATCAGATCAAAAAAAAAAAAA111300.10296955690710166No Hit
GGGGGGGATGCGTGCATTTATCAGATCAAAACCAAAAAAA110450.10218317664321096No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGAAAAAAA108710.10057340998536408No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)