FastQCFastQC Report
Mon 26 Jun 2023
SRR315599.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR315599.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14087578
Total Bases563.5 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGG2365941.6794512158158061No Hit
ATCTGAGGGTCCAGGGTTCAGGTCCCTGTTCGAAAAAAAA937540.665508293902614No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGG877540.6229175802966272No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG764300.5425347068175949No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGG645260.4580347310233171No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAAAA567000.4024822435765751No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCAAAAAAAAAAA547960.38896679045894195No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAA506480.35952241045266975No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCAAAACCAAAAA455010.3229866766310007No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATCAAAAAAAAAA423490.30061235508332235No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAAAAA324140.23008923180407592No Hit
TCGTCGCGCCGTCGGGTCCCGGGGGAAAAAAAAAAAAAAA296630.21056138961573095No Hit
CGGTGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAAA293820.20856672452851727No Hit
GGGGGGGGATGCGTGCATTTATCAGATCAAAACCAAAAAA284950.20227039736709887No Hit
GGGGGGGGATGCGTGCATTTATCAGATCAAAAAAAAAAAA279160.19816039350412112No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAA276290.19612313770330145No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGG268650.1906999201708058No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAAAAAAAAA258650.18360146790314133No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA258460.1834665973100557No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA244860.17381270222603204No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATAAAAAAAAAAA222720.15809672890542292No Hit
ATCTGAGGGTCCAGGGTTCATGTCCCTGTTCGAAAAAAAA203250.14427604234028021No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATCAAAACCAAAA187810.13331603203900627No Hit
GGGGGGGGGATGCGTGCATTTATCAGATAAAAAAAAAAAA167600.11897006000605641No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAAAA165630.11757166490932651No Hit
GGGGGGGATGCGTGCATTTATCAGATCAAAAAAAAAAAAA163310.11592482398322834No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAAAAAA161870.11490264685668466No Hit
GGGGGGGATGCGTGCATTTATCAGATCAAAACCAAAAAAA161870.11490264685668466No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGAAAAAAA159870.11348295640315176No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAAAAA159120.11295057248307694No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGG155920.11067906775742431No Hit
CGGTGGGGGGCGGGGCGGACTGAAAAAAAAAAAAAAAAAA155890.11065777240062132No Hit
GCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGG153310.10882637171556388No Hit
GTCTAGCGGTTAGGATTCCTGAAAAAAAAAAAAAAAAAAA149450.1060863691402454No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCAAAAAAA148540.10544040998388793No Hit
GGGGGGGGGATGCGTGCATTTATCAGACCAAAAAAAAAAA146770.10418398393251133No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAAAA145700.10342444953987122No Hit
TCGACTGCATAATTTGTGGTAGTGGGAGAAAAAAAAAAAA142650.10125942159823356No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)