FastQCFastQC Report
Thu 29 Jun 2023
SRR315600.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR315600.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75080024
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length47
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAAAA17403412.3179814114071142No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAAAAA6693930.8915727038126678No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAAAAAA5297790.7056191138138155No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAAAA5142090.6848812408477652No Hit
ATCTGAGGGTCCAGGGTTCAGGTCCCTGTTCGAAAAAAAAAAAAAAA4772340.635633787224149No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAAAAAAAAAAA4127990.5498120245672804No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAAAAAAAA2346920.3125891382240368No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAAAAAAAAAAAA2313560.30814587912225494No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAAAAAAA2072370.27602148875178834No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCAAAAAAAAAAAAAAAAAA2045410.2724306534585018No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCAAAACCAAAAAAAAAAAA1599290.21301138635757494No Hit
GCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAAAA1387980.1848667496430209No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAAAAAAAAAAA1290410.1718712823000696No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAAAAAAAAAAAAAAAA1180730.15726286928198105No Hit
GGGGGGGGATGCGTGCATTTATCAGATCAAAACCAAAAAAAAAAAAA1132760.15087368645486848No Hit
GGGGGGGGATGCGTGCATTTATCAGATCAAAAAAAAAAAAAAAAAAA1075580.14325781249084313No Hit
ATCTGAGGGTCCAGGGTTCATGTCCCTGTTCGAAAAAAAAAAAAAAA1051340.14002925731616708No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGGAAAAA1039740.13848423916326932No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA989660.13181402286179345No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATCAAAAAAAAAAAAAAAAA956410.1273854147942201No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAAAAA947350.12617870234031892No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGAGAAAA889790.11851221571266414No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGAAAAAAAAAAAAAA822190.10950848923543231No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGAAAAA811010.10801941139496704No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAAAAAAAAAAAA799500.10648638045187625No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)