FastQCFastQC Report
Mon 26 Jun 2023
SRR315601.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR315601.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17915072
Total Bases716.6 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCAT3420391.909224813609457No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGG3127891.7459544678357979No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG3116701.7397083305051746No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATC2490331.3900753510786896No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCA2440921.3624952219003081No Hit
GGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCAT2078791.1603581609942732No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGG1858851.0375900247568082No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA1854031.034899552734145Illumina Single End Adapter 1 (95% over 22bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGG1502560.8387127888740832No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCG1404300.7838651164784601No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGG1384830.7729971724367058No Hit
GGGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCA1210190.6755150076985457No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGC1154890.6446471440360385No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGG1113250.6214041450684653No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT1014370.5662103953587236No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGG999620.5579771044180006No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT925360.5165259732140625No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGC849200.4740142824991158No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACC741350.41381357551898196No Hit
GGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCG718360.4009808054357806No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGG711540.3971739549804768No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGT702990.39240143718093906No Hit
GGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATC684940.3823261218263594No Hit
AGCGCCGAAGGAGCCTCGGTTGGCCCCGGATAGCCGGGTC682670.3810590323053126No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGC655600.36594885021952467No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCA639350.3568782754543214No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC554580.30956057558685784No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATC537560.30006019512508797No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCCTGTA529230.29541047895314065No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGG508980.2841071473226566No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTA498220.2781010313550512No Hit
TTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACC484450.270414765846322No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATC475600.2654747912818882No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAG472300.26363276686803155No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCA459260.2563539794872161No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATC456640.2548915237404572No Hit
AGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAG424320.23685084826898825No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGT419390.23409897543252967No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGT416980.23275373942119795No Hit
GAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGC408770.22817100595520912No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGG400900.2237780568227691No Hit
AAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATTC387540.21632064889273123No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCAC386040.21548336506825091No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGG371640.20744544035324No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACC358570.20014990729593493No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC350220.19548902733966125No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCA339730.18963362246046236No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTG335610.18733388288922312No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGC329190.1837503081204474No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAG311500.17387594088374303No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGT311130.17366941087370455No Hit
TGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGG306640.17116314129242685No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCTGTAG303460.1693880995845286No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCAAAACCCTGTA293040.1635717679504721No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGC277890.15511520132322104No Hit
GTCTAGCGGTTAGGATTCCTCTGTAGGCACCATCAATTCG270050.15073899786727066No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCA265790.14836111180574657No Hit
GGGGGGGGATGCGTGCATTTATCAGATCAAAACCCTGTAG261650.14605020845018094No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTA251690.1404906438556317No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCA246600.1376494607445619No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAG240630.1343170711231303No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCCTGTAGGCACC232590.12982922982391587No Hit
GTCTAGCGGTTAGGATTCCTGGCTGTAGGCACCATCAATT229570.12814349839062886No Hit
AAGCGTCCGTGGGGGTGGGGGCCGGGCCGCCCCTCCCACG214970.11999393583235389No Hit
TGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAG214890.11994928069504829No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCA214290.11961436716525617No Hit
GTCTAGCGGTTAGGATTCCTGCTGTAGGCACCATCAATTC213810.11934643634142247No Hit
GGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCCTGTA212720.11873801009563345No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGC212440.11858171711506378No Hit
GCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGG209300.11682900297581836No Hit
GGGGGGGGATGCGTGCATTTATCAGATCCTGTAGGCACCA208060.11613684834758131No Hit
CGGGCGTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCG200890.11213463166656545No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACC200530.11193368354869017No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTA197070.11000234886022228No Hit
TAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG195680.10922646584953719No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGC190260.1062010802970817No Hit
AGGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCA189330.1056819643259039No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCTGTAGGCACCATCAA187430.10462140481489553No Hit
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAA181190.10113830410505746Illumina Single End Adapter 1 (95% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)