FastQCFastQC Report
Mon 26 Jun 2023
SRR315602.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR315602.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24497475
Total Bases979.8 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA6959212.8407866525019414Illumina Single End Adapter 1 (95% over 22bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGT3358091.370790254914027No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT2123810.8669505734774705No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT1670710.6819927359860557No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTG1554130.6344041579795469No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATC1446030.5902771612176356No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCA1113900.45469992315534563No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATC976900.3987757921989919No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACC879980.3592125310873876No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATC809100.3302789369108449No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTA784300.3201554445917385No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCA647200.26419049310184006No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCA632150.25804700280335013No Hit
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAA614430.2508136042592145Illumina Single End Adapter 1 (95% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACC599060.2445394882533812No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCT576060.23515076553808095No Hit
GTCTAGCGGTTAGGATTCCTCTGTAGGCACCATCAATTCG562050.22943180878845676No Hit
GTCTAGCGGTTAGGATTCCTGGCTGTAGGCACCATCAATT558420.22795002342078113No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCA554980.22654579706684058No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCTGTAGGCACCATCAA541270.22094930191785073No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCA540640.22069213255651857No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGTAGGCACCAT510760.2084949571333372No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAG508730.20766630030237812No Hit
GTCTAGCGGTTAGGATTCCTGCTGTAGGCACCATCAATTC496040.20248617459554505No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCAC452520.1847210783968552No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCAT400790.1636046163941386No Hit
TGGGGAAGGTGGCTCGGGGGGGGCTGTAGGCACCATCAAT378180.154375093759663No Hit
GGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTA348950.14244325180452272No Hit
GGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCAC346700.14152478979976507No Hit
TTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCAC327390.13364234477226733No Hit
ATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTC317260.12950722472418075No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAAT297420.12140843086889568No Hit
TCACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCA294850.12035934315679474No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACC294790.12033485083666785No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGT291150.11884898341563772No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCA288390.1177223366898017No Hit
TTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCCT285810.11666916692434628No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCCTGTA275170.11232586215518131No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTC274520.11206052868714021No Hit
GCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT268120.10944801454027406No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCAC264650.10803154202627006No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTG257560.10513736619794489No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGCTGTAGGCAC256650.10476589934268737No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATC255220.10418216571299695No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAG250460.10223910831626525No Hit
TTGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATT248810.10156556951277632No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)