FastQCFastQC Report
Mon 26 Jun 2023
SRR315603.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR315603.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19226418
Total Bases769 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC5267752.7398499294044267No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGG4301792.237437051457011No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG4156572.1619055613999447No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCA3647211.8969784179247533No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC2814931.4640948719621096No Hit
GGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCAT2569381.3363799746785907No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCAT2462731.2809094236898417No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGG2215461.1522999239900018No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGG2166991.1270898198509989No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGC1753880.9122240034519171No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGG1643960.8550526676367902No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGG1629400.847479754159095No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGG1431490.7445432633369357No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATC1379780.717647977902072No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGT1337040.6954181480918599No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGG1221920.6355422003204133No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACC1127300.5863286650690732No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGG1071200.557150063001855No Hit
GGGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCA1070880.5569836253430046No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGG1069000.5560058040972583No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACC952200.49525605861684685No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA881430.4584473301267038Illumina Single End Adapter 1 (95% over 22bp)
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT811300.42197147695426157No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGG789100.41042486437151215No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGG785850.40873448189881234No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCG773900.40251907557611616No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT763990.39736470932859147No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA761180.395903178636811No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGT748390.38925087345963244No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGC726660.37794871618831966No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAG703440.36587158356798444No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGT679780.35356559916672986No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGG676790.35201044729184605No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAG668620.3477610858143207No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCCTGTAGGCACC656770.3415976912600152No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCA654960.34065627825214245No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCAC650370.3382689380830064No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCAAAACCCTGTA634180.3298482327805418No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCCTGTA614460.31959151205388336No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTA598440.3112592267576831No Hit
GGGGGGGGATGCGTGCATTTATCAGATCAAAACCCTGTAG568470.2956712997709714No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCA565860.29431379261597246No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTA544060.2829752271067861No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCA538710.2801925974978803No Hit
GGGGGGGGATGCGTGCATTTATCAGATCCTGTAGGCACCA527980.27461173474955136No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGC500820.26048533845462013No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGG492500.2561579593245086No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGG468020.24342547842244977No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCA463390.24101733354595745No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGC460310.23941537107952196No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCAC457100.23774579331417842No Hit
AGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAG456240.2372984921060179No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGT414240.21545354938189734No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCA409000.21272813271822136No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC400910.2085203806554086No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATC389750.20271586730299945No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACC386370.20095786953139164No Hit
GAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGC383020.19921547529030106No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGC362200.18838662511134419No Hit
GGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCG360910.18771567329910335No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGC351430.182784957655659No Hit
GCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGG348760.18139624343962563No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTG340090.17688682312014645No Hit
TGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGG338020.17581017951445765No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGC336380.17495718651284914No Hit
GGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATC330920.17211734395871348No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAG328650.17093667681624317No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGG327420.17029693206503677No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAG320940.1669265694733153No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAG319110.16597475411176432No Hit
CGGGCGTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCG311490.1620114573603882No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGG305500.15889595243378146No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAG300150.15611332282487564No Hit
TTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACC299350.15569722867774954No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGC296750.15434492269958971No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATC289940.15080292127217873No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATC289810.15073530597327073No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCAT288610.15011116475258157No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTA288600.15010596357574252No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGCTGT282200.14677721039873365No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCA277000.144072598442414No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAG262090.1363176437753512No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGC259780.13511617192552455No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTG256470.13339458239179028No Hit
GGGGGGGATGCGTGCATTTATCAGATCAAAACCCTGTAGG254540.13239075526184857No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAG245620.1277513055213925No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCAAAACCACTGT232170.12075572267283485No Hit
AGCGCCGAAGGAGCCTCGGTTGGCCCCGGATAGCCGGGTC227700.11843079662576773No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCA224940.11699527181818267No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCTGTAG224180.11659998237841286No Hit
GGGGGGGGATGCGTGCATTTATCAGATCAAAACCACTGTA221060.11497721520462105No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTA219870.11435827516077099No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAG218090.11343246568341539No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTA215360.11201254440634754No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGCTGT214840.11174208321071558No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTA214170.11139360436249748No Hit
TGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAG213050.11081107255652092No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAG210110.10928192656583248No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGT208570.10848094533261474No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGG205060.10665533226209895No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCTGTAGGC204560.10639527342014513No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATCCTGTAGGCAC203200.10568791337003075No Hit
GTGTAATGGTTAGCACTCTGGACTCTGTAGGCACCATCAA199710.10387270265319314No Hit
AGGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACC199100.10355543086600946No Hit
ACCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGG197350.10264522491917111No Hit
TCACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCA196310.10210430252790717No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCA195730.10180263427124076No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)