FastQCFastQC Report
Mon 26 Jun 2023
SRR315604.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR315604.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16889537
Total Bases675.5 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC2320701.3740459551969957No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGG2277481.3484561477321728No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGG2053211.215669796039998No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGG1877381.1115639226818357No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC1702341.0079257945318454No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTA1293420.7658114014611531No Hit
AGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAG1231110.7289187382697346No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGG989610.5859308043790662No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGCTGT912210.5401036156290134No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAG874470.5177584204942978No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAG850570.5036076477407285No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCG842690.4989420373098446No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAG823340.4874852401223314No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTA761330.45077020169350995No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCAT760330.4501781191515197No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTA753260.4459920955796479No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAG738960.437525315229186No Hit
TTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACC735790.43564841357107653No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGT644580.38164456491613713No Hit
TGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAG632720.37462246596813165No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCAC603060.35706129777269796No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCTGTAG588940.3487010922797943No Hit
GGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCG563630.33371548314201865No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTA539190.3192449858157746No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACC525480.3111275341650869No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGCTGT488100.2889954887454878No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCA479640.2839864704402495No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCT458720.2716001036618114No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAG458700.27158826201097164No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGCTGTA453400.2684502245384228No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGT451070.2670706722155853No Hit
AGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGT430950.25515797147073954No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCA430120.25466654296088753No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCCTGTA421040.24929043347961524No Hit
TGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAG411550.2436715701561268No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCA404980.23978158785525028No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTG390710.231332569981048No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCA387180.22924251860782213No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA386490.22883398165384877Illumina Single End Adapter 1 (95% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCAC378420.22405587553998668No Hit
GGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCAT376540.22294276036104485No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCA363740.2153641038235684No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCTGTAGG358770.21242145358987638No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCT349300.20681443191722781No Hit
CGGGCGTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCG345540.20458820155934412No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATC335360.1985608012818824No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCAC334520.19806345194661049No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGC334200.19787398553317356No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGG332820.19705691162522693No Hit
TCGGGCGTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGC297950.17641099338602356No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGG292190.17300059794415915No Hit
TGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTA291070.17233746549712997No Hit
AGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTG288680.17092238822177305No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCCTGTA283520.16786724230510286No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGT280910.16632190687050805No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA278860.16510813765942786No Hit
TCGAGCCCCGAGGGGCTCTCGCTTCTGGCGCCAAGCGTCC278710.1650193252781293No Hit
AGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTA275560.1631542652708597No Hit
TTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGCTG275010.162828619872765No Hit
TGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGT258520.1530651787553442No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGC256700.15198758852892177No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTC256660.15196390522724218No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCC256650.15195798440182226No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGC247200.1463628043800135No Hit
TGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGT245730.14549244304328768No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGC244780.14492996462839686No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGCTGT238370.14113471553423876No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAG237440.14058407877018772No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCTGTAGGC235130.13921636809819002No Hit
TGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGG233380.13818022364970692No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGTAGG233010.13796115310917048No Hit
GAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGC232660.13775392421947388No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGG228040.13501850287547845No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAG225000.13321857194782782No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAG223690.13244294381782046No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGC223090.13208769429262626No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGC218040.12909767745557502No Hit
TGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTG218030.12909175663015512No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCA215670.1276944418310579No Hit
TCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAG214340.12690697205021073No Hit
GAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAG212010.12552741972737322No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAG206220.12209926180924913No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCCTGTA203860.12070194701015191No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATC201600.11936384046525372No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCA197640.11701919359897196No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACTG196840.11654552756537968No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGG189780.11236542481892783No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGG189610.11226477078678948No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC187400.11095626836899082No Hit
GAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTA186300.11030497757280144No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCTGTAG185950.1100977486831048No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCA185260.10968921172913149No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGG183060.1083866301367527No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCTGTAGGCA180020.10658669920910206No Hit
TTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCCTGT176470.10448480618503633No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGG174770.10347826586365275No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)