FastQCFastQC Report
Mon 26 Jun 2023
SRR315605.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR315605.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16714382
Total Bases668.5 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA2682591.6049591304063768Illumina Single End Adapter 1 (95% over 22bp)
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTA1595730.9547047566580684No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCT1120030.6700995585717737No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAG987410.5907547165070177No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACC831360.4973920064768174No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCA733450.4388137114492178No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCAC649370.3885097277302864No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTC555110.33211518080656527No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTG526090.31475288766285225No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCA473090.2830436686202338No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCAC367900.22010984312791226No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCA340690.20383044972886225No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGT326670.19544246386136205No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAG319080.19090146437959837No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGC301000.18008443267600321No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGTAGG289750.17335370221884364No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCA286870.17163063522181077No Hit
TCACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCA273280.16349991282956197No Hit
TCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAG244770.14644274613324024No Hit
ATAACAAAGCAAAGCACTGAAAATGCTTAGATGCTGTAGG241210.14431284387301904No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGCTGTAGGCAC241060.14422310080025694No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCCTGTA233760.13985560459250004No Hit
TAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAA230160.13770177084620897Illumina Single End Adapter 1 (95% over 22bp)
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGG226740.13565562878723245No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGC215440.1288949839724855No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCA213460.1277103754120254No Hit
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAA202970.12143434319019392Illumina Single End Adapter 1 (95% over 22bp)
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAG202940.12141639457564152No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCA201010.12026170037276879No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCAC199990.11965144747798633No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTGTAGGC183860.11000107572029885No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCTGTAG181470.10857116942762227No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGACTGTAGGCA181410.10853527219851743No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACC180880.10821818000809123No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAAT175230.10483785760071775No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)