FastQCFastQC Report
Mon 26 Jun 2023
SRR315606.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR315606.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16775797
Total Bases671 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA2653081.5814926706611911Illumina Single End Adapter 1 (95% over 22bp)
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTA1641480.978481082001648No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCT1130640.673970959472149No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAG1011420.6029042912238387No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACC834350.4973534193338176No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCA728560.4342923319827964No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCAC646860.38559121811023345No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTC549080.32730486664806446No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTG543000.32368059770871094No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCA480680.28653184108033736No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCAC371220.22128307823467344No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCA348320.2076324600255952No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGT334760.1995493865358528No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAG321930.19190146375757886No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGC308860.1841104777316988No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCA292490.17435237205123547No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGTAGG287960.17165205325267108No Hit
TCACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCA276400.1647611734929792No Hit
TCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAG248980.14841619745398685No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGCTGTAGGCAC243940.14541186925425956No Hit
ATAACAAAGCAAAGCACTGAAAATGCTTAGATGCTGTAGG241550.14398719774684923No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCCTGTA230910.13764472710298056No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGG230530.13741821029427098No Hit
TAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAA230180.13720957639151213Illumina Single End Adapter 1 (95% over 22bp)
TCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGC221880.13226197241180254No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCA221020.13174932910788084No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAG201860.12032811317399704No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCA201750.12026254251884426No Hit
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAA199530.11893920747848821Illumina Single End Adapter 1 (95% over 22bp)
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCAC198350.11823581317775841No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTGTAGGC186360.11108861176610567No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCTGTAG181660.10828695650048699No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGACTGTAGGCA180720.10772662544736324No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACC179960.10727359182994406No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAAT177330.10570585707492765No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)