FastQCFastQC Report
Mon 26 Jun 2023
SRR315607.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR315607.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17086206
Total Bases683.4 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGG3029281.7729389426769173No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTA2614601.5302402417482266No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC2219251.2988547603839027No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAG1591240.931300957041019No Hit
AGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAG1558640.9122212385827491No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC1424440.8336783484876631No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGG1418890.8304301142102584No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAG1377380.8061356628850196No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGG1331900.7795176998334212No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCG1323110.7743731990589368No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAG1309540.7664311199338226No Hit
TTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACC1271070.7439158816181896No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTG1268500.742411744304148No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTA1251960.7327314208900443No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGCTGT1136650.665244232686882No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGT1123480.657536260536716No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTA1088320.6369582574387784No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAG1013210.5929988202178997No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGG1009070.590575813027187No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTA869990.5091768178377342No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCTGTAG828470.48487651383812186No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGT805820.4716202063816859No Hit
AGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGT791350.4631513865629385No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGCTGT737250.43148841820120865No Hit
GGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCG729880.42717499718778995No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAG722820.42304300907995607No Hit
CGGGCGTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCG686100.4015519887797209No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCT664020.388629283762586No Hit
TCGGGCGTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGC648810.3797273660401847No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAG616710.3609402813006001No Hit
AGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTG600960.35172231916201874No Hit
TGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAG585010.34238730353596347No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCAT571060.3342228227846486No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCT556440.32566621285029573No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCA513570.3005757978102336No Hit
TGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAG500070.2926746874057353No Hit
TGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTA484150.28335722980280115No Hit
AGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTA472020.27625793578750013No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCA464400.2717981979147389No Hit
TGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGT447310.26179597741008154No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCAC444120.2599289742848705No Hit
TTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGCTG440190.2576288732560055No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTC435290.2547610628128913No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGC430000.2516649980692027No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGTAGG428890.25101535121372176No Hit
TCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAG419170.24532655172248302No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCC415940.24343613789977714No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGG415220.24301474534487058No Hit
TGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTG403490.23614955830451767No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCA396910.2322984985666215No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGG396140.23184784263984645No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCA395000.23118063776124434No Hit
TCGAGCCCCGAGGGGCTCTCGCTTCTGGCGCCAAGCGTCC388470.22735884139521667No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCTGTAGGC386230.2260478423355074No Hit
TGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGT371340.2173332101930645No Hit
GCGGGCGTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGC364060.21307246324900916No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCAC361430.21153320988872545No Hit
GGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCAT337360.19744582267122382No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACTG337210.19735803255561826No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTA335870.19657377418954214No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGCTGTA333040.19491746734178436No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGC328930.1925120181741927No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA320890.18780646797773595Illumina Single End Adapter 1 (95% over 22bp)
GAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAG320070.18732654867909235No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGC307470.17995217896822735No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGT302490.17703754713012357No Hit
GAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTA276540.16184985713036587No Hit
GTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACC273040.15980142109957005No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGG269680.15783492251000603No Hit
TGTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAC265900.1556226115967465No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCCTGTA263360.15413603230582612No Hit
TGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTA254800.1491261430419369No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATC247880.14507609237533484No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA245900.14391726284934175No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCTGT234600.13730374080705804No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCAC232510.13608053186295427No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGG230920.13514995663753557No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCTGTAGG227630.13322442676858748No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT214300.1254228118284422No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGTA212240.1242171609074595No Hit
TCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGG211880.12400646463000622No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACC209260.12247306394409618No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGTA206730.12099233732754948No Hit
TGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAG205780.12043633326204775No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATC204980.11996811931215157No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAG204320.1195818428034872No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAG203300.11898487001736956No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGTAGGCACC202790.11868638362431075No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCTGTAGGCA201030.11765631293453913No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGG198420.11612876492300281No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTA197150.11538547527754259No Hit
TTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACT195170.11422664575154952No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGG194500.11383451656851147No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCTGTAGG188690.11043411275739037No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAG187530.1097552025300409No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGCTGTAGGC185440.10853199358593711No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGCTGTA183430.10735560603682291No Hit
GGGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACC182980.10709223569000631No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAG178710.1045931437324354No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGG177690.10399617094631775No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGTAGGC177380.10381473804073296No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCA174050.10186579747429009No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCA172980.10123956131630392No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC172450.10092936957449769No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCCTGTA171840.10057235643770185No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)