FastQCFastQC Report
Mon 26 Jun 2023
SRR315608.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR315608.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20544504
Total Bases821.7 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTA4410892.1469926944938655No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAG2429881.182739675778982No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTG2157001.0499158315041337No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCT1820670.8862078149951929No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGT1194380.5813622952396417No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTC1015480.4942830452368186No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA992090.48289800522806486Illumina Single End Adapter 1 (95% over 22bp)
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCA878540.42762774900771516No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCAC828040.40304696574811444No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCA814020.3962227562174293No Hit
TCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAG774070.37677716629226No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGC760600.37022066826242195No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCAC754870.36743160117177814No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGTAGG672860.32751338265455326No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGG653670.31817268501590495No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACC435390.21192529155242687No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT393330.1914526629603713No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAG381210.18555327497806715No Hit
TCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGG373520.1818101814480408No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAAT360670.17555546729188495No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGTAGGCACC358920.17470365797100773No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGTA356170.17336510046677203No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGCTGTAGGC353140.17189025347119602No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCCTGTA341000.16598113052522467No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAG330100.16067557532661775No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGCTGT322970.15720506077927215No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCA322120.15679132482341748No Hit
GGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTA319140.15534081523700938No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGCTGTA312040.1518849031351645No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGG300660.14634570880854558No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTGTAGGC285420.1389276664941631No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGTAGGC280810.13668375736888075No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGTAGGCA279520.13605585221234837No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCA278940.1357735382660005No Hit
TACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGT272870.1328189767930148No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGCTGTAGG260390.1267443594647016No Hit
CCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAG255320.12427654617507436No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCTGTAG248180.12080116414589516No Hit
TGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACC247720.12057725998155029No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCTGTAG239260.11645937035033797No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCA230740.11231227582812414No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT229760.11183526260843289No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCA226580.11028740338535309No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGCTGTAGG223020.10855457985259706No Hit
TGGCCGTGATCGTATAGTGGTTAGTACTCTGCGCTGTAGG209310.10188126225875299No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)