FastQCFastQC Report
Mon 26 Jun 2023
SRR315609.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR315609.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17103202
Total Bases684.1 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTA3924802.294774978392935No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAG2212281.2934887864857119No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTG1942221.135588528978375No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCT1871291.0941167624635433No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGT1110560.6493287046484044No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTC1014000.5928714400964218No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA822090.48066438085687113Illumina Single End Adapter 1 (95% over 22bp)
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCA821110.4800913887352789No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCA746460.4364445909017504No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCAC718180.4199096753929469No Hit
TCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAG676760.3956919879681009No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGC669170.39125422245495317No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCAC664030.3882489372457859No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGTAGG637880.3729594025726879No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGG605830.3542202214532694No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACC409300.23931191364049842No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT380560.22250804264604954No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAG348600.20382148325208344No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGTA338220.19775244424991298No Hit
TCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGG333800.19516813284436446No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGTAGGCACC315510.1844742288607712No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCA313990.18358550638646495No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAG309570.18100119498091644No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGCTGTAGGC279480.1634079981046824No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAAT279430.16337876381276442No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGTAGGC262480.15346833885257274No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTGTAGGC260060.15205339912374305No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCCTGTA253300.14810092285643356No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGTAGGCA251300.14693155117971476No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGCTGTA249180.14569201720239286No Hit
CCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAG235740.13783383953484266No Hit
TACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGT229670.13428479649600114No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT228310.13348962375583237No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGG217530.12718671041831817No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGCTGTAGG209160.12229288995125005No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCTGTAG202050.11813577364051481No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCTGTAG200780.11739322262579839No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGCTGTAGG200500.11722951059105774No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCA194220.11355768352616079No Hit
TGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACC187480.10961690097561849No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGCTGT187100.10939472035704191No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCCTGTAGG180780.10569950585861056No Hit
TGGCCGTGATCGTATAGTGGTTAGTACTCTGCGCTGTAGG175190.10243111202218158No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCA172870.1010746408771878No Hit
GGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTA172120.10063612649841824No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)