FastQCFastQC Report
Mon 26 Jun 2023
SRR315610.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR315610.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14364423
Total Bases574.5 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGT2309391.6077151167157915No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA1262500.8789075621067411Illumina Single End Adapter 1 (95% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCA1116210.7770656712072598No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACC1083930.7545934841935523No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT879810.6124924057165402No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCA685340.47710931375384863No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT668240.46520490241759105No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTG667160.4644530448595116No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACC569780.3966605550393496No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTA558490.3888008588997971No Hit
TCACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCA516900.35984738126968274No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAG503250.35034473713284553No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCT438330.3051497439194042No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCA418060.2910384914173023No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATC415340.28914492423399113No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCAT389730.2713161538058299No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGCTGTAGGCAC388070.27016052089248555No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATC365200.254239240935748No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATC307360.21397309171416073No Hit
GGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCAC304540.21200990809028666No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACC300220.20900247785796897No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCCTGTA291010.20259080368212495No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCA287270.19998714880507207No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTGTAGGC285330.19863658985815164No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCAC260920.18164321671674527No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCA252090.17549608501504027No Hit
ATAACAAAGCAAAGCACTGAAAATGCTTAGATGCTGTAGG242070.1685205176706367No Hit
GTCTAGCGGTTAGGATTCCTGGCTGTAGGCACCATCAATT229640.15986719410866693No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGTAGGCACCAT221250.1540263747454388No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAG220240.15332324869575337No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGC216050.15040631983616745No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCA212030.14760773892553847No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCA211430.147190040282161No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGG203430.1416207250371282No Hit
AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGT202980.14130745105459508Illumina DpnII expression PCR Primer 2 (100% over 40bp)
AACAAAGCAAAGCACTGAAAATGCTTAGATGCTGTAGGCA201460.14024928115803886No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGG200020.13924680441393295No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGACTGTAGGCA192970.13433884535424778No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACC188470.13120610552891682No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGTGCTGTAGGCA185820.1293612698539997No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAG175350.12207242852706301No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTGCTGTAGGCAC173130.12052694354656641No Hit
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAA170980.11903019007446383Illumina Single End Adapter 1 (95% over 22bp)
CTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCA169730.11815998456742745No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCTGTAG168700.11744293522962948No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCA167810.11682334890861959No Hit
GGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTA160460.11170654052724568No Hit
AAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCTGTA157280.10949273771734515No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCAC156940.10925604181943126No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG150450.10473793482689837No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCGCTGTAGGCAC144880.10086029908754428No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)