FastQCFastQC Report
Mon 26 Jun 2023
SRR315611.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR315611.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14423793
Total Bases576.9 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGT2339431.6219242747036096No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA1270020.8805034847629885Illumina Single End Adapter 1 (95% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCA1141180.7911788528856453No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACC1091820.7569576185681534No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT926390.6422651794850356No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCA714500.4953620729304698No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT695280.4820368678335858No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTG680970.4721157603967278No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTA567880.3937105863901402No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACC564660.3914781638921191No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAG517200.3585741975082421No Hit
TCACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCA513520.35602285751050367No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCT443440.3074364697274843No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATC428400.2970092540845532No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCA426460.2956642541944411No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCAT391950.2717385087265187No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGCTGTAGGCAC391730.2715859829657844No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATC387820.26887518421818724No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATC323250.22410887344265132No Hit
GGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCAC309260.2144096216577706No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCCTGTA306080.2122049311162466No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACC305770.21199000845339364No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCA295260.20470343688376558No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTGTAGGC281760.19534390156597503No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCAC263010.18234454695793262No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCA260270.18044490793787735No Hit
ATAACAAAGCAAAGCACTGAAAATGCTTAGATGCTGTAGG239770.1662322802330843No Hit
GTCTAGCGGTTAGGATTCCTGGCTGTAGGCACCATCAATT233870.16214181664975363No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGC230380.1597222034453767No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGTAGGCACCAT226270.15687274491529377No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAG224730.15580506459015322No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCA216710.15024480731247322No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCA212260.14715962715216446No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGG206200.14295823574284516No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGG202360.14029596791911808No Hit
AACAAAGCAAAGCACTGAAAATGCTTAGATGCTGTAGGCA195310.13540821058649413No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGACTGTAGGCA194920.13513782401064683No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACC194540.13487437042392386No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGTGCTGTAGGCA192800.1336680303162975No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCTGTAG180590.12520285059554032No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAG180150.12489779907407157No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCA176180.12214540239172873No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCA174140.1207310726103737No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGTGCTGTAGGCAC173570.12033589223028922No Hit
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAA170210.11800640788452801Illumina Single End Adapter 1 (95% over 22bp)
AATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGT169350.11741017081983914Illumina DpnII expression PCR Primer 2 (100% over 40bp)
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCAC163360.11325731033438985No Hit
GGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTA160130.11101795484724442No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG155960.10812689838241578No Hit
AAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCTGTA155210.10760692419809408No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCGCTGTAGGCAC144850.10042434746533038No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)