FastQCFastQC Report
Mon 26 Jun 2023
SRR315612.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR315612.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15697206
Total Bases627.8 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC2873731.830727073340313No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC2529801.6116243871680096No Hit
AAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATTC1915981.220586644527695No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGG1805981.1505104793808527No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGG1389970.8854887933559642No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCCTGTA1347760.8585986576209804No Hit
GGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCAT1206450.76857626764916No Hit
TGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCAT1170480.7456613616461426No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGG1108120.7059345465683511No Hit
TTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCCTGT867820.5528499785248406No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCA854590.5444217270258159No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTCG811160.5167543829137491No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGG808400.5149961082246102No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCA750110.47786211125725175No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGC707580.45076811758729546No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGC694060.4421551198347018No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCAT688880.43885516951233233No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGG622110.39631893726819917No Hit
TGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCA598950.381564719224555No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA587570.3743150214120908Illumina Single End Adapter 1 (95% over 22bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGT578860.36876626324455447No Hit
GGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCA559400.35636915257403134No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATC555930.3541585680916719No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAG522680.33297645453592184No Hit
GATCGTCGGACTGTAGGCACCATCAATTCGTATGCCGTCT514520.3277780771941198No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAG499980.3185152822738008No Hit
AGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAG487200.31037370599583136No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGT463890.29552392954516876No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGCTGT463370.2951926604008382No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCAC433080.2758962327435851No Hit
TTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACC416960.26562688926933875No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCG408390.260167318948353No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG407050.2593136638456551No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA403510.2570584854400203No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCA385250.24542584202564455No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTG376030.23955218527424563No Hit
TGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAG373570.2379850273991435No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGC352890.22481070835153721No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGC342500.21819169602539457No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTA341220.21737626428550405No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTA326380.20792235255114827No Hit
TGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGG326370.20791598199068037No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGCTGT323350.2059920727293762No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACC320760.20434209756819144No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC317870.20250100559296985No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCT314320.20023945662686723No Hit
GGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCG310310.19768486187924142No Hit
TTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGCTG290760.18523041616450725No Hit
TCGGGCGTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGC290710.18519856336216778No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCA289840.1846443246014609No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGG286750.18267582141688146No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAG276300.17601858572793147No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCAC273140.17400548862007673No Hit
CGGGCGTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCG269640.17177579245631355No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGG260380.16587665346304303No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCTGTAG251550.16025144856989199No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCAC240010.15289982178994146No Hit
GAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGC233730.14889910981610358No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGG228010.1452551492284678No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAG226660.144395123565302No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGC225230.14348413341839306No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAG223810.142579513831952No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTA222780.14192334610375884No Hit
TGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAG218960.13948979200502307No Hit
GATCGTCGGACTGTAGAACTCTGCACCATCAATTCGTATG214590.13670585708055308Illumina DpnII expression Sequencing Primer (96% over 27bp)
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGT213360.135922278143002No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACC202770.1291758546075015No Hit
ATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTC201790.1285515396816478No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCTGTAG201470.12834768174667519No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCT197450.12578671643858147No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGG197010.12550641177799413No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTC196090.12492032021494781No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAGG193940.12355064971435045No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCAT193420.12321938057001992No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAG192540.12265877124884518No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCA179980.1146573473011694No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCTGTAGG176730.11258691514910361No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAA171780.10943348771749573No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCA168800.10753506069806308No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTA165050.10514610052260256No Hit
ACTGTAGAACTCTGAACCTGTAGGCACCATCAATTCGTAT163110.10391021179183098Illumina DpnII expression PCR Primer 2 (95% over 24bp)
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAG162790.10370635385685836No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCA161440.10284632819369255No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTG161060.10260424689591256No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGCTGTAGGC157920.1006038909089936No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGC157150.1001133577529657No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)