FastQCFastQC Report
Mon 26 Jun 2023
SRR315614.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR315614.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22292861
Total Bases891.7 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAGGAATTATAACAAAGAAGTAACACGTACTGTAGG8743023.921892304446702No Hit
ACTGCTAATGTAAAAGAGAAATTATATATGTGCCTCTGTA7132423.1994188632854255No Hit
TACTGCTAATGTAAAAGAGAAATTATATATGTGCCTCTGT6514882.922406415219653No Hit
AACTTAAGGAATTATAACAAAGAAGTAACACGTACTGTAG4338861.9463002079454943No Hit
ACTGCTAATGTAAAAGAGAAATTATATATGTGCCCTGTAG2378021.066718174935016No Hit
TTACTGCTAATGTAAAAGAGAAATTATATATGTGCCTCTG2346711.052673319947583No Hit
TGTAAAAGAGAAATTATATATGTGCCTCTGTAGGCACCAT2260271.0138985749742935No Hit
ATGTAAAAGAGAAATTATATATGTGCCTCTGTAGGCACCA2217470.9946996036085274No Hit
TACTGCTAATGTAAAAGAGAAATTATATATGTGCCCTGTA2108910.9460023996022762No Hit
TTAACTTAAGGAATTATAACAAAGAAGTAACACGTACTGT1844360.8273321221533656No Hit
TTTACTGCTAATGTAAAAGAGAAATTATATATGTGCCTCT1395520.6259941243073287No Hit
TGGATAAGTATTTGTTAGGCCTTATAAGAAAAAAGCTGTA1272570.5708419390404847No Hit
TAGTATTATGAGTTTCTTAGATGGTAACATACTGTAGGCA1039480.4662838026936067No Hit
TAGTATTATGAGTTTCTTAGATGGTAACATATCTGTAGGC967400.4339505817579897No Hit
ACTTAAGGAATTATAACAAAGAAGTAACACGTCTGTAGGC899210.4033623140609902No Hit
TTACTGCTAATGTAAAAGAGAAATTATATATGTGCCCTGT875090.392542706833367No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGG849960.38127003976743945No Hit
TTTTTGGTACATAAATAATCCTATCACCCTGTAGGCACCA846860.37987946006571344No Hit
TTTACTGCTAATGTAAAAGAGAAATTATATATGTGCCCTG720940.32339500972979646No Hit
TTTAACTTAAGGAATTATAACAAAGAAGTAACACGTACTG695680.3120640280312159No Hit
GGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCAT683600.3066452529354577No Hit
AAATCCCCACTTTGCATACAATACCAGTAAGCACTGTAGG627670.281556503671736No Hit
AAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATTC605000.27138732888524264No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA597330.26794676555871405Illumina Single End Adapter 1 (95% over 22bp)
TGGATGGCGCTAAAGCGGATTACCGATACCAAGCCACTGT569650.25553023454459256No Hit
TAGTATTATGAGTTTCTTAGATGGTAACATATACTGTAGG567560.2545927146811708No Hit
ATGTAAAAGAGAAATTATATATGTGCCCTGTAGGCACCAT546890.24532068808933946No Hit
AAGGAATTATAACAAAGAAGTAACACGTACTGTAGGCACC535450.24018900041587304No Hit
ATAAGTATTTGTTAGGCCTTATAAGAAAAAAGCTGTAGGC527290.23652863578165223No Hit
AAATCCCCACTTTGCATACAATACCGGTAAGCACTGTAGG523580.23486442588055428No Hit
AAATGTAAAAGAGAAATTATATATGTGCCTCTGTAGGCAC505830.2269022356529294No Hit
TGGATGGCGCTAAAGCGGATTACCGATACCAAGCCCTGTA505290.2266600056403707No Hit
ATTAACTTAAGGAATTATAACAAAGAAGTAACACGTACTG496460.22269909636093815No Hit
ACGGAAGGGCACCACCAGGCGTGGAGCTTGCGGCTCTGTA483640.21694837643315498No Hit
AACTTAAGGAATTATAACAAAGAAGTAACACGTCTGTAGG461850.20717394685231294No Hit
ACGCTTGGATAAGGTGCCTAAATATTCGCTCATGCTGTAG452180.20283623533112238No Hit
TGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCAT442060.19829666546613287No Hit
TAATGTAAAAGAGAAATTATATATGTGCCTCTGTAGGCAC441930.19823835083347982No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGG434890.19508038918826973No Hit
TAAGGAATTATAACAAAGAAGTAACACGTACTGTAGGCAC422730.189625728164725No Hit
TGAAAGATATGATGAATTGTTTGGACAAGCTGTAGGCACC411840.18474075624479064No Hit
ATGGATGGCGCTAAAGCGGATTACCGATACCAAGCCCTGT401710.18019670063882784No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC398990.17897657909408757No Hit
AACTATGCCGACTAGGTGTTGGATGAAAGTGCTGTAGGCA397310.17822297461057152No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC397210.17817811720083843No Hit
TTGGATAAGTATTTGTTAGGCCTTATAAGAAAAAAGCTGT384600.17252159783349477No Hit
TGTAAAAGAGAAATTATATATGTGCCCTGTAGGCACCATC381620.17118484702344847No Hit
ATGGCGCTAAAGCGGATTACCGATACCAAGCCCTGTAGGC378420.1697494099119893No Hit
AAACTATGCCGACTAGGTGTTGGATGAAAGTGCTGTAGGC378030.16957446601403023No Hit
TTAAATCCCCACTTTGCATACAATACCGGTAAGCACTGTA363390.16300734122910468No Hit
ATTAAATCCCCACTTTGCATACAATACCGGTAAGCACTGT352370.1580640546765173No Hit
TAATATACGCATAATTAATGTAATATGCTGTAGGCACCAT347970.15609032864826097No Hit
TTTTTTTGGTACATAAATAATCCTATCACCCTGTAGGCAC345300.15489263580838727No Hit
TTCTTTCTTTTTTTCTCCCCCTCTTTTCGCCCTGTAGGCA333870.14976543387589417No Hit
TCTTTCTTTTTTTCTCCCCCTCTTTTCGCCCTGTAGGCAC333250.14948731793554898No Hit
TTGGCGCTAAAGCGGATTACCGATACCAAGCCCTGTAGGC324830.1457103240360221No Hit
GGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCA317780.1425478766498387No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATC313870.14079395192927457No Hit
AATCCCCACTTTGCATACAATACCAGTAAGCACTGTAGGC310760.13939888648657522No Hit
AATGTAAAAGAGAAATTATATATGTGCCTCTGTAGGCACC310680.13936300055878875No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGC309430.13880228293712504No Hit
TTTTGGTACATAAATAATCCTATCACCCTGTAGGCACCAT306240.1373713315666392No Hit
TAACTTAAGGAATTATAACAAAGAAGTAACACGTACTGTA304200.13645624040808402No Hit
TAGGCTGCACGCGTGCTACACTGATATATATAACGCTGTA302550.1357160931474879No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGG300920.1349849173688384No Hit
AGGCTGCACGCGTGCTACACTGATATATATAACGCTGTAG300580.13483240217574585No Hit
AGGAATTATAACAAAGAAGTAACACGTACTGTAGGCACCA286920.12870488000620467No Hit
TGGATAAGTATTTGTTAGGCCTTATAAGAAAAAAGTCTGT281320.1261928650611512No Hit
ACTATGCCGACTAGGTGTTGGATGAAAGTGCTGTAGGCAC276760.12414736717732192No Hit
TAATCCCCACTTTGCATACAATACCAGTAAGCACTGTAGG275910.1237660791945906No Hit
ATGGATGGCGCTAAAGCGGATTACCGATACCAAGCCACTG266020.1193296813719872No Hit
TAAACAATACTATTTCGTTCTTTTTTGTTTCCTCTGTAGG264040.11844150465927186No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTCG261850.11745912738611701No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCA256860.11522074264043543No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCA253630.11377184830605636No Hit
TCTTCTTTCTTTTTTTCTCCCCCTCTTTTCGCCCTGTAGG250510.1123722971223837No Hit
AAGGGAAACAGGTTGATATTCCTGTGCCACTGTAGGCACC249750.11203138080841217No Hit
TAAACTATGCCGACTAGGTGTTGGATGAAAGTGCTGTAGG245430.11009354070794233No Hit
TTTTTGGTACATAAATAATCCTATCACCTCTGTAGGCACC236090.10590385863887097No Hit
TGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCA235630.10569751455409875No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCAT234860.10535211249915388No Hit
TTGAAAGATATGATGAATTGTTTGGACAAGCTGTAGGCAC231860.10400639020716095No Hit
ACGGAAGGGCACCACCAGGCGTGGAGCTTGCGGCCTGTAG224950.10090674319460385No Hit
TGCTAATGTAAAAGAGAAATTATATATGTGCCTCTGTAGG224250.1005927413264722No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)