FastQCFastQC Report
Mon 26 Jun 2023
SRR315615.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR315615.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16391233
Total Bases655.6 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGT1659231.012266740397138No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA1532500.9349510192430308Illumina Single End Adapter 1 (95% over 22bp)
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTG989640.6037617792389383No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACC867820.5294415618397957No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCT759130.4631317241357011No Hit
ATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTC559930.34160334368988593No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCAC510470.31142867653702444No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAG475900.2903381338060413No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCA456950.2787770755256789No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTC430170.2624390733753831No Hit
GTCTAGCGGTTAGGATTCCTGGCTGTAGGCACCATCAATT394720.24081165828098472No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTA379920.23178244125991013No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCAC345780.21095423388832313No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT344630.21025263932249633No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCA341860.20856271154220063No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGCTGTAGGC327370.19972262001278365No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTG321240.19598281593581154No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTG288270.17586840477467436No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAG280200.1709450411692641No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCCTGTA279850.1707315123883603No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAG271770.16580204796063847No Hit
GTCTAGCGGTTAGGATTCCTGCTGTAGGCACCATCAATTC260650.15901793355020943No Hit
CCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAA252250.15389324280851843No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGTAGGCACC237240.14473590851890153No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTA230390.14055684523549875No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT222400.13568228820858078No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAAT220610.13459024101481568No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGCTGTAGG215150.1312591920327165No Hit
GTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATT213180.13005733003734374No Hit
TCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAA208150.12698861641464068No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGCTGTAGGCA204920.12501805080801426No Hit
GTCTAGCGGTTAGGATTCCTCTGTAGGCACCATCAATTCG199970.1219981437638035No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGG199550.12174190922671896No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCAC198530.12111962535094217No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATC194710.11878911122793506No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACC192690.11755674512100463No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCTGTAG190480.11620846339015496No Hit
AGTTATGGTTCCTTTGGTCGCTCGCTGTAGGCACCATCAA187720.11452463643217077No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCA184060.11229173546614826No Hit
AAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATTC178960.10918031608726446No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCAC174780.10663017236104202No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCA173460.10582486381591916No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGCTGTA171670.10473281662215406No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)