FastQCFastQC Report
Mon 26 Jun 2023
SRR315616.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR315616.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19449857
Total Bases777.9 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG4599302.364696048922108No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGG3252931.672469879855672No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCA3040131.563060335096551No Hit
GGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCAT2397571.232692867613371No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGG2159571.1103269293959332No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGG1996411.0264394231793066No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCAT1806400.9287471882184019No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGG1544610.7941497976051957No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCA1270290.6531102002446599No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC1204890.6194852743647421No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGG1189500.6115726197884128No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGG1074240.5523125439945393No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATC1060520.5452585075561224No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACC1057430.5436698069296859No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAG1009480.519016669377055No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGG996930.5125641797777741No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACC966330.4968314162926751No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGT959550.4933455294812707No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGG943180.4849290151593403No Hit
GGGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCA891770.45849694421917864No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCA841080.4324350559492545No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA823680.4234889747518452Illumina Single End Adapter 1 (95% over 22bp)
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTA763580.39258900463895446No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCCTGTAGGCACC744980.3830259523244824No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGC744650.3828562852672901No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGG681520.350398463083816No Hit
GGGGGGGGATGCGTGCATTTATCAGATCCTGTAGGCACCA679930.3495809763537079No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGT648220.33327751458532573No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTA642180.3301720932961101No Hit
GGGGGGGGATGCGTGCATTTATCAGATCAAAACCCTGTAG604580.3108403316281451No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGC603170.31011539056559645No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCA596850.30686600934906616No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCCTGTA585050.3007991266979495No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCA570900.293524008942585No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGC568750.29241860235784767No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCAAAACCCTGTA560050.28794556175914304No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCAC550790.2831846013058091No Hit
CGGGCGTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCG547280.28137996078840066No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCAC540430.2778580839951677No Hit
AGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAG529520.2722487882558725No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGC510580.26251092745823273No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCA496970.2555134467055465No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTA490230.25204812559804424No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT479740.24665476974972106No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATT469440.24135910099493274No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACC451130.2319451500337509No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCG441820.22715848245053938No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT439070.22574459030727062No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAG417480.21464425162611736No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGC404000.2077136094111129No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGT403710.20756450805782273No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCAT403120.2072611639252669No Hit
GAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGC402140.2067573041796657No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGG388250.19961586349966481No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAG381010.19589347109338645No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGG362430.18634070163086547No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTG360040.18511190082271556No Hit
TGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGG358750.18444865687187315No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAG355260.18265429920641577No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGC354200.18210930805301037No Hit
GTGTAATGGTTAGCACTCTGGACTCTGTAGGCACCATCAA347750.17879308829879828No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGC347330.17857714840782635No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGCTGT342760.1762275167370125No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAG341730.17569794986153367No Hit
GGGGGGGATGCGTGCATTTATCAGATCAAAACCCTGTAGG328490.1688907018699418No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAG315190.1620526053224967No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTA314030.1614561999093361No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGCTGT312610.1607261174208119No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGT311310.16005773204399396No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCAC311000.15989834783875276No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGC299590.15403198080068148No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCA286090.14709105573372597No Hit
GCCTGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCA278140.14300362208318548No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTG266020.1367722138008521No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATC265360.1364328796864676No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTA254770.1309881095783892No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGTAGGCA253950.13056651264839633No Hit
GCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGG253920.13055108837046978No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCT250540.12881328639074313No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAGG249370.128211739551607No Hit
GGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATC247760.12738396996954784No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCTGTAGGC247340.1271680300785759No Hit
TTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACC242720.12479269127788446No Hit
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCA239130.12294691935267185No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAAT235950.12131194589245567No Hit
GGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCG227890.11716795655618445No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCA226590.1164995711793665No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCTGTAG225930.11616023706498202No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCA222630.11446356649305955No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTA220640.11344042272393055No Hit
TGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAG218170.11217049050797649No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATCCTGTAGGCAC214580.11032471858276388No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCC212350.10917818059022234No Hit
AGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGT207100.10647893195307298No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAG206730.10628869919197864No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTA204550.1051678683293147No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAG202050.10388251183543407No Hit
TCACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCA199690.10266913530521073No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCAC198430.10202131563229488No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)