FastQCFastQC Report
Mon 26 Jun 2023
SRR315617.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR315617.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24822364
Total Bases992.8 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCA2192360.8832196643317293No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGT1894760.7633277797392706No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACC1760960.709424775174516No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCA1513360.6096760163536398No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA1288730.5191810095122286Illumina Single End Adapter 1 (95% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCAC1101280.44366443099456604No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCA973520.39219471602301864No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACC869060.3501116976610286No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCA833080.3356167043557979No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTG765620.30843959906477886No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATT713040.28725708800338273No Hit
GTGTAATGGTTAGCACTCTGGACTCTGTAGGCACCATCAA603420.24309529906176544No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCAC563160.22687605419048723No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT538420.21690923555870828No Hit
GCCTGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCA525220.21159145035501054No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGTAGGCA458420.18468023432417638No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT440090.17729576441631426No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCA437360.17619594974918584No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCT416920.16796143993376297No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCA407300.1640859025353105No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATC360750.14533265244196725No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCA355630.1432699963629572No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAAT326980.13172798529584048No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTG322230.12981438834754014No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGTAGGCACCAT304740.12276832295264062No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCA301630.12151542052964819No Hit
GCGCAGCGGAAGCGTGCTGGGCCCTGTAGGCACCATCAAT294340.11857855279215146No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCAC286330.11535162404354396No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGC269790.10868827803830448No Hit
TCACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCA269180.10844253190389118No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCA251310.10124337875312761No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)