FastQCFastQC Report
Mon 26 Jun 2023
SRR315618.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR315618.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25726565
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length40
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCA2771741.0773844079067687No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACC2435090.9465274512940224No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCA2004450.7791362741197669No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA1746700.6789480056898385Illumina Single End Adapter 1 (95% over 22bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGT1643370.638783296565243No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCAC1528240.5940318888277545No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCA1266280.49220717962153127No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACC1149660.44687660400834706No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCA1072990.4170747241227113No Hit
GTGTAATGGTTAGCACTCTGGACTCTGTAGGCACCATCAA878950.34165074116968197No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATT866160.3366792263172328No Hit
GCCTGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCA727260.282688341797671No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCAC689240.26790984338562107No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTG645790.2510206862050958No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGTAGGCA608070.23635879877472954No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCA561080.2180936320103364No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCA508430.19762840472484375No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCA505340.19642731161350147No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAAT474190.18431920468200866No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT469060.18232515689521706No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCA420010.16325926138992905No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCAC373120.1450329649527638No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCT368540.1432527039657257No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGTAGGCACCAT351490.13662531317336768No Hit
GCGCAGCGGAAGCGTGCTGGGCCCTGTAGGCACCATCAAT347580.13510548337875655No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT341720.13282768220320126No Hit
TCACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCA336720.13088416584180593No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGC334250.12992406875927665No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATC330920.12862968686258736No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGCTGTAGGCAC311250.12098389349685822No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCA284090.11042671262175886No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCA280400.10899239754704913No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGCTGTAGGCAC279740.10873585338734494No Hit
GTCTAGCGGTTAGGATTCCTGGCTGTAGGCACCATCAATT275450.10706831634926778No Hit
GTATAGTGGTGAGTATCCCCGCCTGTAGGCACCATCAATT267860.1041180585126697No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTG266520.10359719612781575No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCAT263370.1023727808201367No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCAC261970.10182859623894601No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)