FastQCFastQC Report
Mon 26 Jun 2023
SRR315619.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR315619.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25600179
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length40
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCA2692571.0517778020224No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACC2482970.9699033745037486No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCA1987160.7762289474616564No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAA1859730.7264519517617436Illumina Single End Adapter 1 (95% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCAC1556330.607937155439421No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGT1337060.5222854105824807No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCA1254480.49002782363357694No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACC1152580.45022341445346925No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCA1081150.42232126580052426No Hit
GTGTAATGGTTAGCACTCTGGACTCTGTAGGCACCATCAA951030.3714934961978196No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATT834850.32611100102073504No Hit
GCCTGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCA725810.2835175488421389No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCAC668840.26126379819453605No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGTAGGCA589090.23011167226604157No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCA552030.21563521098817315No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCA524580.20491262971247196No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTG520790.20343217131411465No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAAT501420.19586581796947594No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCA486690.19011195195158595No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCA411750.16083871913551853No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT404320.15793639567910833No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCAC380540.14864739812952088No Hit
GCGCAGCGGAAGCGTGCTGGGCCCTGTAGGCACCATCAAT336210.1313311129582336No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGTAGGCACCAT333640.13032721372768527No Hit
TCACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCA328800.12843660194719733No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGC321100.12542881047823923No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGCTGTAGGCAC304090.11878432568772272No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTCT300300.11730386728936544No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATC288780.11280389875398919No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCA283620.11078828784751857No Hit
GTCTAGCGGTTAGGATTCCTGGCTGTAGGCACCATCAATT278390.10874533338223924No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT277950.10857345958401306No Hit
GTATAGTGGTGAGTATCCCCGCCTGTAGGCACCATCAATT277450.10837814844966513No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGCTGTAGGCAC272530.10645628688768154No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCAT265890.10386255502354104No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCA265870.10385474257816714No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)