FastQCFastQC Report
Mon 26 Jun 2023
SRR315620.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR315620.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11418377
Total Bases456.7 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGG669440.5862829717393286No Hit
GTCTAGCGGTTAGGATTCCTGAAAAAAAAAAAAAAAAAAA377110.3302658512676539No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGG348440.30515720404046914No Hit
GTCTAGGGGTATGATTCTCGCAAAAAAAAAAAAAAAAAAA302440.26487126848237713No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGG272290.23846646506767116No Hit
GTCTAGTGGTTAGGATTCGGCGAAAAAAAAAAAAAAAAAA269250.23580409019600596No Hit
CGGTGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAAA247140.21644056769188827No Hit
GTCTAGCGGTTAGGATTCCTAAAAAAAAAAAAAAAAAAAA240650.21075674765336613No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG236220.20687703690288034No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGG231780.20298856834031664No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAAAAAAAAA220260.19289956882663797No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA209360.18335355366178574No Hit
TCGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAAAA204930.17947384291129992No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAAAA197500.17296678853746028No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAAAAA195250.1709962808199449No Hit
TCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCAA187060.16382363272818895No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAAAAAAAAA183890.16104740629951173No Hit
TCGTCGCGCCGTCGGGTCCCGGGGGAAAAAAAAAAAAAAA170630.14943454748428783No Hit
GTATAGTGGTGAGTATCCCCGAAAAAAAAAAAAAAAAAAA157990.13836467301789038No Hit
GTATAGTGGTGAGTATCCCCGCAAAAAAAAAAAAAAAAAA157250.13771659492412977No Hit
GTCTAGTGGTTAGGATTCGGCAAAAAAAAAAAAAAAAAAA148120.12972071249705627No Hit
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAAAAA134060.11740722871560468No Hit
CGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCAAA131850.11547175224640069No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAAAAAAAAA118550.1038238621828654No Hit
CCGGTGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAA115550.10119651855951158No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)