FastQCFastQC Report
Mon 26 Jun 2023
SRR315621.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR315621.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19727446
Total Bases789 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGG1367780.693338610583448No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGG786620.39874396310601995No Hit
GTCTAGCGGTTAGGATTCCTGAAAAAAAAAAAAAAAAAAA710910.36036595918194375No Hit
GTCTAGGGGTATGATTCTCGCAAAAAAAAAAAAAAAAAAA573870.2908992882302149No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGG546550.2770505619429905No Hit
GTCTAGTGGTTAGGATTCGGCGAAAAAAAAAAAAAAAAAA520650.26392164500158816No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG485200.24595175675553743No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGG474490.24052277218247103No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAAAAAAAAA466420.2364320247030457No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAAAAA456630.23146939548079362No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAAAA443770.22495055872919384No Hit
TCGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAAAA441840.22397222630846386No Hit
GTCTAGCGGTTAGGATTCCTAAAAAAAAAAAAAAAAAAAA419610.2127036616904185No Hit
CGGTGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAAA396720.20110053779896295No Hit
TCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCAA378050.1916365656253729No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA367480.1862785481709087No Hit
TCGTCGCGCCGTCGGGTCCCGGGGGAAAAAAAAAAAAAAA313750.15904238186737402No Hit
GTATAGTGGTGAGTATCCCCGCAAAAAAAAAAAAAAAAAA309020.15664470707460054No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAAAAAAAAA306050.15513919034425439No Hit
GTATAGTGGTGAGTATCCCCGAAAAAAAAAAAAAAAAAAA296730.15041480787730963No Hit
GTCTAGTGGTTAGGATTCGGCAAAAAAAAAAAAAAAAAAA277230.14053010207200667No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAAAAA249000.12622008951386815No Hit
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAAAAA242790.12307219089587167No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAAAAAA231770.11748606484590048No Hit
CCGGTGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAA231290.11724274901069302No Hit
CGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCAAA210100.10650136870226384No Hit
CCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCAA199910.10133597628400554No Hit
ATCTGAGGGTCCAGGGTTCAGGTCCCTGTTCGAAAAAAAA199010.10087975909299156No Hit
TCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCAAA198250.10049450902057976No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)