FastQCFastQC Report
Mon 26 Jun 2023
SRR315622.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR315622.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19029625
Total Bases761.1 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGG1386720.7287164092828945No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGG789460.4148584115556665No Hit
GTCTAGCGGTTAGGATTCCTGAAAAAAAAAAAAAAAAAAA728130.38262971550937025No Hit
GTCTAGGGGTATGATTCTCGCAAAAAAAAAAAAAAAAAAA584020.3069004249952377No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGG526930.27689983381175404No Hit
GTCTAGTGGTTAGGATTCGGCGAAAAAAAAAAAAAAAAAA520230.27337900773136625No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGG474200.24919040706267204No Hit
TCGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAAAA471660.2478556461307041No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAAAAAAAAA470850.2474299940224781No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAAAAA466460.24512306469517925No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG466060.24491286612321578No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAAAA454910.23905358092973458No Hit
GTCTAGCGGTTAGGATTCCTAAAAAAAAAAAAAAAAAAAA447180.23499149352654083No Hit
CGGTGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAAA405780.2132359413283236No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA402470.21149654814532604No Hit
GTATAGTGGTGAGTATCCCCGCAAAAAAAAAAAAAAAAAA338580.17792258123846372No Hit
TCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCAA336780.17697668766462818No Hit
TCGTCGCGCCGTCGGGTCCCGGGGGAAAAAAAAAAAAAAA333290.17514270512424707No Hit
GTATAGTGGTGAGTATCCCCGAAAAAAAAAAAAAAAAAAA313220.16459599177598086No Hit
GTCTAGTGGTTAGGATTCGGCAAAAAAAAAAAAAAAAAAA275810.14493717033309905No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGAAAAA261060.13718609799194678No Hit
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAAAAA250150.13145293194164362No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAAAAAAAAA247560.1300918961881803No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCAAAAAA243900.1281685792547147No Hit
CCGGTGGGGGGCGGGGCGGACTGTAAAAAAAAAAAAAAAA241020.12665514953657783No Hit
GGATGCGTGCATTTATCAGATCAAAAAAAAAAAAAAAAAA192930.10138402622227186No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)