FastQCFastQC Report
Tue 27 Jun 2023
SRR315623.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR315623.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77904761
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAAAAAAA4099930.5262746393638252No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAAAAAAAA2356390.3024706025348053No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAAAAAAAAAAAAAA1676040.21513961130052114No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAAAAAAAAAAAAAAA1644960.21115012470162126No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAAAAAAA1563350.20067451333301697No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAAAAAAAAAA1338130.1717648552955576No Hit
TCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCAAAAAAAAAAAA1180710.15155813134450152No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAAAAAAAAAAAAAAAAAAA1142190.14661363250957155No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAAAAAAAAA1130790.14515030730920284No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1052750.13513294777966137No Hit
TCGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAAAAAAAAAAAAAA1007530.12932842448486556No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAAAAAAAAAAAAAAAAAAA900030.11552952456910816No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)