FastQCFastQC Report
Mon 26 Jun 2023
SRR315624.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR315624.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11036184
Total Bases441.4 Mbp
Sequences flagged as poor quality0
Sequence length40
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCG902960.8181813568893016No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA539100.4884840629695917No Hit
GGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCG498020.45126105182733456No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGG464040.4204714238182328No Hit
TTGGGGAAGGTGGCTCGGGGGGAAAAAAAAAAAAAAAAAA443510.4018689793501087No Hit
TTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACC438170.39703035034573547No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGG400780.3631508862121183No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAA296980.2690966370259865No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAA296720.26886104834787095No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAA278760.25258730735188906No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAA265800.24084411785812923No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAAAAAA235370.21327118141560522No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGG211510.19165138964700118No Hit
TGGGGGTGGGGGCCGGGCCGCAAAAAAAAAAAAAAAAAAA199330.18061496618758802No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAAAA195340.17699958608881475No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA180950.1639606588654194No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAA176210.15966569604131284No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAAAAAAA167270.15156506995533964No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAAAAA163530.14817621743167747No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA162980.14767785676643302No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGG161290.14614653035868194No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAAAAA161040.14592000278357084No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAAAA160320.14526760336725086No Hit
GGGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAA159490.14451553181788196No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAAAAAAA154370.13987624707960652No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAA136320.12352095615658457No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGG136070.12329442858147344No Hit
GGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAAAAAAA133980.1214006580535446No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGAAAAAAAAAAAA126190.11434205881308249No Hit
TTGGGGGTGGGGGCCGGGCCGCAAAAAAAAAAAAAAAAAA124620.11291946564138473No Hit
TTGGGGAAGGTGGCTCGGGGGGGGAAAAAAAAAAAAAAAA123300.1117234000447981No Hit
GGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAA120770.1094309409846737No Hit
TGTGGGGAAGGTGGCTCGGGGGGAAAAAAAAAAAAAAAAA115310.1044835787442471No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAAAA114970.104175501242096No Hit
GTCTAGCGGTTAGGATTCCTGAAAAAAAAAAAAAAAAAAA111570.10109472622058494No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)