FastQCFastQC Report
Mon 26 Jun 2023
SRR315625.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR315625.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17257062
Total Bases603.9 Mbp
Sequences flagged as poor quality0
Sequence length35
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGC2396991.3889907795428909No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGT2181671.2642186717530481No Hit
GGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGC1755181.017079268765448No Hit
TTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCG1297030.7515937533283475No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCC1059010.613667610396254No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCG965580.559527456064074No Hit
TTGGGGAAGGTGGCTCGGGGGGAAAAAAAAAAAAA898250.5205115447809135No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA893710.5178807377524632No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAA809200.4689094818109827No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC711600.412352925428442No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGT644000.3731805564585675No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAA641040.37146531663385113No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAA615990.35694952014427483No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCC605800.3510446911531059No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCA586670.33995937431296247No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA559820.32440052657862617No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCAA523100.30312228118552276No Hit
GGGGGGGGGGATGCGTGCATTTATCAAAAAAAAAA447410.25926197634336595No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTC441960.2561038489633983No Hit
TGGGGGTGGGGGCCGGGCCGCAAAAAAAAAAAAAA428550.2483331171899365No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAA389740.225843773407084No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCC365290.21167566066576105No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAA364750.21136274529233307No Hit
AGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCC355740.20614169433939566No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGGCGC339900.19696284338550793No Hit
GGGGGGGGGATGCGTGCATTTATCAAAAAAAAAAA338790.19631962845123926No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAA319780.18530384836074645No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGT298000.1726829282991508No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAA293000.16978556373037312No Hit
AGCGCCGAAGGAGCCTCGGTTGGCCCCGGATAGCC277630.16087906504595045No Hit
TGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCC276300.1601083660706556No Hit
TTGGGGGTGGGGGCCGGGCCGCAAAAAAAAAAAAA274490.15905952009675806No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGA269640.1562490764650437No Hit
GGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAA269390.1561042082366048No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGC260450.15092372038763027No Hit
GGGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCG259760.15052388407713896No Hit
TTGGGGAAGGTGGCTCGGGGGGGGAAAAAAAAAAA255830.1482465555260797No Hit
GGGGGGTGGGGGCCGGGCCGCAAAAAAAAAAAAAA242330.1404236711903799No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCG240040.13909667821787972No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA229270.13285575493673257No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAAA217050.12577459593063986No Hit
GTCTAGCGGTTAGGATTCCTGAAAAAAAAAAAAAA216210.12528783868308524No Hit
GCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGT214260.12415786650126191No Hit
GGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAA209830.12159080149332488No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAA207780.12040288202012601No Hit
TGTGGGGAAGGTGGCTCGGGGGGAAAAAAAAAAAA203030.1176503856797872No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGAAAAAAA200690.11629441906159924No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCG200280.11605683516695947No Hit
GTCTAGCGGTTAGGATTCCTAAAAAAAAAAAAAAA195680.11339125976368399No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAA193530.11214539299910957No Hit
TTGGGGAAGGTGGCTCGGGGAGAAAAAAAAAAAAA186910.10830928231004791No Hit
TGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCG183900.10656506883964373No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCA182590.10580595932262397No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGAAAAA179570.10405595112308226No Hit
TTGGGGGGGGGGATGCGTGCATTTATCAGAAAAAA177160.1026594214009314No Hit
CGGGCGTGGGGGTGGGGGCCGGGCCGCCCCTCCCA176200.10210312740372607No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)