FastQCFastQC Report
Tue 27 Jun 2023
SRR315627.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR315627.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76656433
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length47
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAAAA3276160.4273822654910124No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2678800.3494553418628284No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAAAAAA2600450.3392344123290996No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAAAAAAAAAAAAAAAAAA2472510.3225443584102068No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAAAAAAAAAAAAA2080130.27135752585826683No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAA1927290.25141921226624253No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAAAAAAAAAAA1899750.2478265587964418No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTAAAAA1765260.2302820429956609No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAAAAAAAAAAAA1635810.2133950062612488No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAAAAA1569280.20471602168078964No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCAAAAAAAAAAAAAA1473880.192270882210238No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAAAAAAAAAAAAAA1448730.18899000948817954No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCAAAA1382280.18032146108337704No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAAAAAAAAAAAA1325800.17295352106978418No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA1296090.16907778633529685No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAAAAAAAAAAAA1187040.15485197439332976No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAA1179590.15388010553530454No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAAAAAAA1077630.1405791996609078No Hit
TTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTAAAA1056610.13783709450712375No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAAAA1005660.13119055513579664No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAAAAAAAAAAA996270.12996560901809767No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAAAAAAAAAAAAAA951770.12416048630908771No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA936080.1221136913584278No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAAAAAAAAAAAAAAAAAAA924790.12064088606888348No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAAAAAAAAAAA872320.11379605936008005No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAA865960.11296638339537662No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAAAAAAAAAAA842160.10986162113752411No Hit
GGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAAAAAAAAAAA835850.10903846778260606No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAAAAAAAAAAAA824010.10749391378542228No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGAAAAAAAAAAAAAAAAAAA795010.10371080010988772No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCAAAAAA784440.1023319204012532No Hit
TTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCGCTCAAA777760.10146049973392317No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)