FastQCFastQC Report
Sun 2 Jul 2023
SRR3182054.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3182054.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49351988
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAG3302140.6690996926000226No Hit
GGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAGA3091830.6264854011554711No Hit
TTAGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAAA2272080.46038266989366267No Hit
TTTCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAA2086900.42286037190639614No Hit
TTTCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA1739310.35242957183406676No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAA1639070.3321183333080726No Hit
CGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAGA1458980.2956274020815534No Hit
GGCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAG1372130.2780293268023975No Hit
GGGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA1345030.27253815996226943No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAA1293890.2621758620949576No Hit
TTACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA1229500.24912876863238012No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG1144080.23182044865143023No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1142620.23152461457074436No Hit
GCGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAG1125030.22796042177672762No Hit
ATAGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAAA1076170.2180601113778841No Hit
TTACGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAA1027250.20814764341408087No Hit
CCGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAG1011910.20503935930605266No Hit
GGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAAA977790.19812575736564048No Hit
GGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAAA894100.18116798050769506No Hit
GCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAGA816610.16546648536225125No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAG799780.16205628839105732No Hit
GGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAAG790220.16011918304081285No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA786170.15929854740603358No Hit
TATCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAA755320.15304753275592464No Hit
TATCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA749250.151817592434169No Hit
TAAGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAAA737700.14947726117942808No Hit
TTATCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA716030.1450863539681522No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAGA697610.1413539815255264No Hit
GGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAGAT679220.1376276878653804No Hit
GGCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAA647740.13124901878319473No Hit
CCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAGA638690.12941525273510765No Hit
CGCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAG623440.12632520497451896No Hit
GGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG621570.12594629419994186No Hit
TAACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA609570.12351478120800322No Hit
GCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAG585690.11867607035404532No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA569090.11531247738186352No Hit
ATACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA563570.11419398140557174No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA557450.11295390977968305No Hit
GGCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAA556080.1126763120464367No Hit
AATCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAA554490.11235413657500483No Hit
GCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG551670.11178273102189927No Hit
TTAGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAAA551200.11168749676304833No Hit
AAACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA547530.11094385903968043No Hit
ATACGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAA543320.11009080323167528No Hit
AATCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA528390.1070655958175383No Hit
AAAGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAAA522240.10581944540916974No Hit
GCGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAA513910.10413157014059901No Hit
AAACGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAA500380.10139003924218817No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)