FastQCFastQC Report
Sun 2 Jul 2023
SRR3182055.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3182055.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28791381
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAG2031100.7054541774151091No Hit
GGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAGA1719990.5973975336577291No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAA908630.31559097495184407No Hit
GGCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAG878740.3052093958257855No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG834760.28993399100932327No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAA812770.28229628860109207No Hit
CGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAGA782360.27173409986829045No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG689170.2393667743829308No Hit
GGGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA673810.23403184446067382No Hit
GCGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAG657850.2284885188383287No Hit
GGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAAA603530.20962176145701383No Hit
CCGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAG577090.20043845760646214No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA505200.17546917947423224No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAG487980.169488222881702No Hit
GCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAGA479550.1665602632954633No Hit
GGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG454910.15800214654517614No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAGA448080.1556299088258392No Hit
TTTCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA443430.15401484215015598No Hit
GGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAGAT435030.1510973023489217No Hit
GGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAAG433650.15061799223871894No Hit
TTTCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAA430800.14962811266330017No Hit
TTAGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAAA422260.146661947198712No Hit
GGCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAA421160.14627988841521705No Hit
GCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG419940.14585615049170444No Hit
CGCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAG397830.13817676894345568No Hit
CCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAGA367970.1278056096024015No Hit
GCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAG367300.1275729010706364No Hit
GGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAAA360100.12507215266957844No Hit
GGCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAA350510.121741294729836No Hit
CCGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG335490.11652445570429566No Hit
TTACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA315220.10948415430298394No Hit
GGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAAG310560.10786561436563255No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA310150.10772321063723897No Hit
GGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAGA302580.10509395155446No Hit
GGGGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAAAAAAAAAAAAA293380.10189855081977485No Hit
GCGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAA291010.10107538780442661No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)