FastQCFastQC Report
Sun 2 Jul 2023
SRR3182056.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3182056.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19328294
Total Bases966.4 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAG946360.4896241747978378No Hit
GGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAGA722280.373690507812019No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAA514760.2663245912960554No Hit
GGGCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAA479230.24794221362733826No Hit
GGCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAG472880.24465687452808818No Hit
TTTCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAA446140.23082223397471086No Hit
TTAGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAAA382990.19814992466484627No Hit
CGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAGA311980.16141103813921703No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG311920.1613799955650509No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG303020.15677534706373983No Hit
GCGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAG302800.1566615242917973No Hit
GGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAAA301280.1558751124129217No Hit
TTTCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA290690.1503960980725976No Hit
GGCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAA280800.1452792470975452No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAA271510.1404728218641542No Hit
CCGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAG254820.13183781248360565No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAG252890.13083927634792808No Hit
TTACGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAA242180.1252981768592717No Hit
GCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAGA226550.11721158628899167No Hit
TTACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA224550.11617683381678695No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAGA212780.11008731551786205No Hit
GGGGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAAAAAAAAAAAAA211930.10964754571717503No Hit
GGCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAG206080.10662089473597618No Hit
GCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAG197100.1019748561357769No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)