FastQCFastQC Report
Thu 28 Sept 2023
SRR3199149.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3199149.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42906847
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG22462155.235096859948716TruSeq Adapter, Index 3 (100% over 49bp)
GACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGT2267960.5285776416990043No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAGATCGGAAGAGCACACGTCT1440130.33564106912819763Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGCGATCCGTTGAAACTCAGATCGGAAGAGCACACGTCTGAACTCCAG937100.21840337044574726Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGTGACCGGTTGGGAATACCTCGTGCGCTAAGCAAGATCGGAAGAGCACA846090.19719230359667306No Hit
TGACCTCAGATTAGTCGAGAGCACTCGCTGAGATCGGAAGAGCACACGTC824440.19214648888090052No Hit
GCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAACT701640.16352634813739636Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGAGATCGGAAGAGCACACGT610680.14232693444009065No Hit
GACTTGGGCCAACGTCTATCTGAGGGTAGATCGGAAGAGCACACGTCTGA603290.14060459860870225Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGTAGATCGGAAGAGCACACG602560.14043446259288173No Hit
GCTTAGCGTCATACCGCGTAGAATAAACAGATCGGAAGAGCACACGTCTG595390.13876340062927486Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACG593890.13841380607621903No Hit
CGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTGA532860.12418996902755404Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAAGATCGGAAGAGCACACGTC503090.1172516824645726No Hit
ATCCGGCGCGCTTGTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGT482460.11244359204487805Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCTGGTAGAGATAAATCGATGCAGATCGGAAGAGCACACGTCTGAACTC459860.10717636744550352Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTG446470.10405565340189178Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)