FastQCFastQC Report
Thu 28 Sept 2023
SRR3199151.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3199151.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21886546
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG10637844.860447144103962TruSeq Adapter, Index 3 (100% over 49bp)
GACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGT1249630.5709580671157524No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAGATCGGAAGAGCACACGTCT857670.39187087811845694Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGCGATCCGTTGAAACTCAGATCGGAAGAGCACACGTCTGAACTCCAG586250.2678586196286979Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGACCTCAGATTAGTCGAGAGCACTCGCTGAGATCGGAAGAGCACACGTC535260.24456120211932939No Hit
GGTGACCGGTTGGGAATACCTCGTGCGCTAAGCAAGATCGGAAGAGCACA478080.21843556310803908No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAGAGATCGGAAGAGCACACGT353740.1616244061534424No Hit
GCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAACT353380.16145992154266828Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCTTAGCGTCATACCGCGTAGAATAAACAGATCGGAAGAGCACACGTCTG352880.16123147069437088Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTGA349100.15950438228124256Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGTAGATCGGAAGAGCACACG347530.15878704661758872No Hit
GACTTGGGCCAACGTCTATCTGAGGGTAGATCGGAAGAGCACACGTCTGA340270.15546994030031053Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACG333230.15225335235628318No Hit
ATCCGGCGCGCTTGTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGT304970.13934131041051429Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAAGATCGGAAGAGCACACGTC297310.1358414434145982No Hit
AGCTGGTAGAGATAAATCGATGCAGATCGGAAGAGCACACGTCTGAACTC287590.13140035892369678Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGAAC265400.1212617102762583Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAACTCCA258510.11811365758672017Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTGAA245430.11213738339526026Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTG245150.11200945092021371Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCCGAGTGTCGTAAACGGTAGAGCAGATCGGAAGAGCACACGTCTGAAC233210.10655404466287188Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTGCGATCCGTTGAAACTCATCTCAGCAGATCGGAAGAGCACACGTCTG225090.10284400288652217Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTTGGGCCAACGTCTATCTGAGAGATCGGAAGAGCACACGTCTGAACT219750.10040414782670597Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)