FastQCFastQC Report
Thu 28 Sept 2023
SRR3199152.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3199152.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21898497
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG10524084.80584580759127TruSeq Adapter, Index 3 (100% over 49bp)
GACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGT1295150.5914332842112406No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAGATCGGAAGAGCACACGTCT888470.40572190867711144Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGCGATCCGTTGAAACTCAGATCGGAAGAGCACACGTCTGAACTCCAG585120.2671964199186821Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGACCTCAGATTAGTCGAGAGCACTCGCTGAGATCGGAAGAGCACACGTC536060.24479305588872147No Hit
GGTGACCGGTTGGGAATACCTCGTGCGCTAAGCAAGATCGGAAGAGCACA493390.22530770034126088No Hit
GCTTAGCGTCATACCGCGTAGAATAAACAGATCGGAAGAGCACACGTCTG368190.16813482678742747Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGAGATCGGAAGAGCACACGT367800.1679567323730026No Hit
GCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAACT366140.16719868948083513Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGTAGATCGGAAGAGCACACG361000.16485149642918415No Hit
GACTTGGGCCAACGTCTATCTGAGGGTAGATCGGAAGAGCACACGTCTGA350440.16002924766937202Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTGA347970.15890131637801444Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACG336030.15344888738254503No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAAGATCGGAAGAGCACACGTC311490.14224263884411792No Hit
ATCCGGCGCGCTTGTGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGT304500.13905063895481046Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCTGGTAGAGATAAATCGATGCAGATCGGAAGAGCACACGTCTGAACTC289330.1321232228860273Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGAAC267380.12209970392031927Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAACTCCA257880.11776150664586707Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTGAA252160.11514945523430214Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTG251960.11505812476536631Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCCGAGTGTCGTAAACGGTAGAGCAGATCGGAAGAGCACACGTCTGAAC231070.10551865728501823Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTGCGATCCGTTGAAACTCATCTCAGCAGATCGGAAGAGCACACGTCTG223490.10205723251235006Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGTGACATCCGGCGCGCTTGTGGCGAGATCGGAAGAGCACACGTCTGAA223180.1019156702854995Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTTGGGCCAACGTCTATCTGAGAGATCGGAAGAGCACACGTCTGAACT223040.10185173895724442Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)