FastQCFastQC Report
Thu 28 Sept 2023
SRR3199153.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3199153.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18654657
Total Bases951.3 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACCTCAGATTAGTCGAGAGCACTCGCTGAGATCGGAAGAGCACACGTC1981641.062276299156827No Hit
GACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGT1877461.00642965453613No Hit
GGAGCTCCGGCGACAATGAAATACCACTACAGATCGGAAGAGCACACGTC1539450.8252362935432155No Hit
GGAGCTCCGGCGACAATGAAATACCACAGATCGGAAGAGCACACGTCTGA1495990.8019391618939978Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGGTGGGAGCTCCGGCGACAATGAAATACCACAGATCGGAAGAGCACAC1403660.7524448184707979No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAGATCGGAAGAGCACACGTCT1364900.7316671649336678Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGACCGGTTGGGAATACCTCGTGCGCTAAGCAAGATCGGAAGAGCACA1170430.6274197376022513No Hit
TTGTGACATCCGGCGCGCTTGTGGCGAGATCGGAAGAGCACACGTCTGAA1010020.5414304856958775Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG909250.4874118028543757TruSeq Adapter, Index 5 (100% over 49bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGAGATCGGAAGAGCACACGT858960.4604533870550394No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAAGATCGGAAGAGCACACGTC838120.4492819138942088No Hit
GAGTGATGGCCGCATCGGCGACTCAGGAGATCGGAAGAGCACACGTCTGA787920.4223717434204231Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGAGCTCCGGCGACAATGAAATACCACTACAGATCGGAAGAGCACACGT770720.4131515256485284No Hit
GGAGCTCCGGCGACAATGAAATACCACTACTAGATCGGAAGAGCACACGT767810.4115915934557253No Hit
AGGTGGGAGCTCCGGCGACAATGAAATACCACAGATCGGAAGAGCACACG761410.408160814749904No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAGTAGATCGGAAGAGCACACG680240.36464889169498No Hit
GGAGCTCCGGCGACAATGAAATACCAGATCGGAAGAGCACACGTCTGAAC641450.3438551563826663Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAAACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAA597320.3201988650876829Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGTGACATCCGGCGCGCTTGTGGCAGATCGGAAGAGCACACGTCTGAAC591310.31697714945924765Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGAGCTCCGGCGACAATGAAATACCACTACTAGATCGGAAGAGCACACG585030.3136106978541605No Hit
TAGGTGGGAGCTCCGGCGACAATGAAATACCACTACAGATCGGAAGAGCA552850.2963603136739529No Hit
TGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGA541360.29020099377865805Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGTAAACGATGTCTACTTGGAGGTTGTGGCAGATCGGAAGAGCACACGT527140.2825782323416614No Hit
GTGGGAGCTCCGGCGACAATGAAATACCACTACTAGATCGGAAGAGCACA521690.2796567098499854No Hit
GCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTGAA519900.27869716393070104Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGCGATCCGTTGAAACTCATCTCAGCGAGATCGGAAGAGCACACGTCT492500.2640091425964037Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGAGCTCCGGCGACAATGAAATACCACAGATCGGAAGAGCACACGTCTG489130.26220262318411963Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTAGCGTCATACCGCGTAGAATAAACAGATCGGAAGAGCACACGTCTG473780.2539741148818764Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAAC465510.24954090552294794Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTGA456660.2447967818438045Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGAGCTCCGGCGACAATGAAATACCACTACAGATCGGAAGAGCACAC454500.2436388940305898No Hit
TCATTGATGCCATGCTTCCAGGAAAAGCCTAGATCGGAAGAGCACACGTC454390.24357992752158347No Hit
GGAGCTCCGGCGACAATGAAATAGATCGGAAGAGCACACGTCTGAACTCC424850.2277447395575271Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTAGAACATCGAAACTCTGAACGCAAATTGAGATCGGAAGAGCACACGTC419800.22503764073496502No Hit
ATTGATGCCATGCTTCCAGGAAAAGCCTAGATCGGAAGAGCACACGTCTG405240.21723261917922157Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAAC382120.20483893110444218Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAAATTGCCTTGATGTGGGGGATAACCAAGATCGGAAGAGCACACGTCT373970.20047004884624786Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAA344400.18461877910700797Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTG330640.1772426048894922Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTTGGGCCAACGTCTATCTGAGGGTAGATCGGAAGAGCACACGTCTGA329000.1763634678461255Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGCAGAGCGCTATGTGGCATATAGCTCGAGAGATCGGAAGAGCACACGT328300.1759882264251763No Hit
TGACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACG327120.17535567660129048No Hit
GTGGGAGCTCCGGCGACAATGAAATACCACAGATCGGAAGAGCACACGTC321570.17238054819233609No Hit
AAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGAAC321240.17220364866531718Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTAAACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTG313840.16823681078671132Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGTGGTGAGTCTGGATAAACTAGCTGATCAGATCGGAAGAGCACACGTC306590.16435038178402314No Hit
AGCTCCGGCGACAATGAAATACCACTACAGATCGGAAGAGCACACGTCTG303980.1629512673430554Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAAGTGATCCCTGGAATCGGTTTGCCCGAGATCGGAAGAGCACACGTCTG303620.16275828604085296Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTTAAACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCT300190.16091960307820186Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGTAAACGATGTCTACTTGGAGGTTGTGGCAGATCGGAAGAGCACACG295060.158169619521817No Hit
GGAGCTCCGGCGACAATGAAATACCAAGATCGGAAGAGCACACGTCTGAA290450.1556983867352801Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTCCGGCGACAATGAAATACCACTACTAGATCGGAAGAGCACACGTCT285120.15284119134433832Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGGTGGGAGCTCCGGCGACAGATCGGAAGAGCACACGTCTGAACTCCAG283240.1518334000995033Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTGACATCCGGCGCGCTTGTGGCGAGATCGGAAGAGCACACGTCTGAAC269270.14434465345570277Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTTAGCGTCATACCGCGTAGAATAGATCGGAAGAGCACACGTCTGAACT263410.14120334670318516Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCTGCGCATAACCGCCCGATGCGAGAGCAGATCGGAAGAGCACACGTCTG262160.14053327273720442Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGAGCGCAACCCTTATCCTTGTTTGCAGATCGGAAGAGCACACGTCTG260590.13969165983593265Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAACT255500.13696311864645916Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTGCGATCCGTTGAAACTCATCTCAGCAGATCGGAAGAGCACACGTCTG255140.13677013734425672Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTCTCGCGTCAAGATATGCCTAGGTGGGAGATCGGAAGAGCACACGTC247410.13262639993863193No Hit
CTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAACTCC246110.13192952301401198Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGACCTCAGATTAGTCGAGAGCACTCGCTAGATCGGAAGAGCACACGTCT241250.12932427543427896Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATTGCGATCCGTTGAAACTCATCTCAGCGAGATCGGAAGAGCACACGTC236100.12656357069443838No Hit
CTCCGGCGACAATGAAATACCACTACAGATCGGAAGAGCACACGTCTGAA233370.1251001291527365Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGAATCAACTGTAGACGACTTTAAGCAGATCGGAAGAGCACACGTCTGA231160.12391543838088258Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTTGGGCCAACGTCTATCTGAGAGATCGGAAGAGCACACGTCTGAACT228300.12238230914671872Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCCGGCGACAATGAAATACCACAGATCGGAAGAGCACACGTCTGAACTC227560.12198562535885812Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGATCGGAAGAGCACACGT223330.11971809505797937No Hit
AACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAACT220620.11826537469973315Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTAAACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGA219330.11757385836684105Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCTTGCGGATGGAGCAGACGAGAGATCGGAAGAGCACACGTCTGAACTC218190.11696275090986663Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGCGCTATGTGGCATATAGCTCGAGAGATCGGAAGAGCACACGTCTGAA218020.11687162085049325Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTGGTAGAGATAAATCGATGCAGATCGGAAGAGCACACGTCTGAACTC213060.11421276735348176Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGAAAAGCTCAAAGCCGAATCGTTTTAGATCGGAAGAGCACACGTCTGAA208250.11163432273238795Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGCGAGTTTAAGTACATTTGAATGTGTCTAGATCGGAAGAGCACACGTC202650.10863239136479433No Hit
TGAGTCTGAATATTCGTGCATGGAATAATGAAAGATCGGAAGAGCACACG201750.10814993810928822No Hit
GACTTGGGCCAACGTCTATCTGAAGATCGGAAGAGCACACGTCTGAACTC198480.10639702461428265Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCATTGATGCCATGCTTCCAGGAAAAGCCTCAGATCGGAAGAGCACACGT193410.10367920460826485No Hit
GTGTGAGTCCCATCTGACGAGCGCGGCGAGAGATCGGAAGAGCACACGTC188420.10100426933606982No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)