FastQCFastQC Report
Thu 28 Sept 2023
SRR3199154.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3199154.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18699497
Total Bases953.6 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACCTCAGATTAGTCGAGAGCACTCGCTGAGATCGGAAGAGCACACGTC1988861.0635901061937656No Hit
GACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGT1884311.0076795113793704No Hit
GGAGCTCCGGCGACAATGAAATACCACTACAGATCGGAAGAGCACACGTC1554060.8310704828049653No Hit
GGAGCTCCGGCGACAATGAAATACCACAGATCGGAAGAGCACACGTCTGA1507460.8061500263884103Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGGTGGGAGCTCCGGCGACAATGAAATACCACAGATCGGAAGAGCACAC1412850.7555550825778897No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAGATCGGAAGAGCACACGTCT1371060.7332068878644169Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGACCGGTTGGGAATACCTCGTGCGCTAAGCAAGATCGGAAGAGCACA1178110.6300222941825654No Hit
TTGTGACATCCGGCGCGCTTGTGGCGAGATCGGAAGAGCACACGTCTGAA1006210.5380946877875913Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG900290.481451452945499TruSeq Adapter, Index 5 (100% over 49bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGAGATCGGAAGAGCACACGT867340.4638306581187719No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAAGATCGGAAGAGCACACGTC841780.45016184125166575No Hit
GAGTGATGGCCGCATCGGCGACTCAGGAGATCGGAAGAGCACACGTCTGA788160.42148727316034224Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAGCTCCGGCGACAATGAAATACCACTACTAGATCGGAAGAGCACACGT769930.4117383478282865No Hit
GGGAGCTCCGGCGACAATGAAATACCACTACAGATCGGAAGAGCACACGT769630.4115779157054331No Hit
AGGTGGGAGCTCCGGCGACAATGAAATACCACAGATCGGAAGAGCACACG763890.40850831442150554No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAGTAGATCGGAAGAGCACACG684720.3661702772005044No Hit
GGAGCTCCGGCGACAATGAAATACCAGATCGGAAGAGCACACGTCTGAAC649140.3471430274300961Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAAACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAA596760.3191315787798998Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGTGACATCCGGCGCGCTTGTGGCAGATCGGAAGAGCACACGTCTGAAC593770.3175326052887947Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGAGCTCCGGCGACAATGAAATACCACTACTAGATCGGAAGAGCACACG585160.3129282033629033No Hit
TAGGTGGGAGCTCCGGCGACAATGAAATACCACTACAGATCGGAAGAGCA555600.2971202915244191No Hit
TGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGA535880.286574553315525Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGAGCTCCGGCGACAATGAAATACCACTACTAGATCGGAAGAGCACA526310.28145676859650287No Hit
GCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTGAA524400.28043535074766984Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGTAAACGATGTCTACTTGGAGGTTGTGGCAGATCGGAAGAGCACACGT524250.2803551346862432No Hit
ATTGCGATCCGTTGAAACTCATCTCAGCGAGATCGGAAGAGCACACGTCT496700.2656221180708765Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGAGCTCCGGCGACAATGAAATACCACAGATCGGAAGAGCACACGTCTG488150.26104980256955573Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTAGCGTCATACCGCGTAGAATAAACAGATCGGAAGAGCACACGTCTG478410.25584110631425006Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAAC471650.25222603581262104Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCATTGATGCCATGCTTCCAGGAAAAGCCTAGATCGGAAGAGCACACGTC456130.243926347323674No Hit
GTGGGAGCTCCGGCGACAATGAAATACCACTACAGATCGGAAGAGCACAC452490.2419797708997199No Hit
CGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTGA452280.24186746841372256Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAGCTCCGGCGACAATGAAATAGATCGGAAGAGCACACGTCTGAACTCC422900.2261558158489504Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTAGAACATCGAAACTCTGAACGCAAATTGAGATCGGAAGAGCACACGTC422570.2259793405138117No Hit
ATTGATGCCATGCTTCCAGGAAAAGCCTAGATCGGAAGAGCACACGTCTG410220.21937488478968176Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAAC384980.2058771955202859Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAAATTGCCTTGATGTGGGGGATAACCAAGATCGGAAGAGCACACGTCT381500.20401618289518697Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAA345610.18482315326449691Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGCAGAGCGCTATGTGGCATATAGCTCGAGAGATCGGAAGAGCACACGT329870.17640581455212404No Hit
GACTTGGGCCAACGTCTATCTGAGGGTAGATCGGAAGAGCACACGTCTGA328430.17563574036242793Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACG326810.1747694068990198No Hit
TTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTG324340.17344851575419382Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGAGCTCCGGCGACAATGAAATACCACAGATCGGAAGAGCACACGTC323790.1731543901956293No Hit
AAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGAAC317560.16982274977770792Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTAAACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTG313930.1678815210911823Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGTGGTGAGTCTGGATAAACTAGCTGATCAGATCGGAAGAGCACACGTC307560.16447501234926268No Hit
CAAGTGATCCCTGGAATCGGTTTGCCCGAGATCGGAAGAGCACACGTCTG305000.16310599156758065Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTCCGGCGACAATGAAATACCACTACAGATCGGAAGAGCACACGTCTG301710.1613465859536222Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGTAAACGATGTCTACTTGGAGGTTGTGGCAGATCGGAAGAGCACACG300500.1606995097247803No Hit
CGTTAAACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCT300380.16063533687563897Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAGCTCCGGCGACAATGAAATACCAAGATCGGAAGAGCACACGTCTGAA295720.15814329123398346Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTCCGGCGACAATGAAATACCACTACTAGATCGGAAGAGCACACGTCT283870.1518062223812758Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGGTGGGAGCTCCGGCGACAGATCGGAAGAGCACACGTCTGAACTCCAG280280.14988638464446397Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCTGCGCATAACCGCCCGATGCGAGAGCAGATCGGAAGAGCACACGTCTG267730.14317497417176514Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTAGCGTCATACCGCGTAGAATAGATCGGAAGAGCACACGTCTGAACT266470.14250115925578105Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTGACATCCGGCGCGCTTGTGGCGAGATCGGAAGAGCACACGTCTGAAC265690.1420840357363623Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACGAGCGCAACCCTTATCCTTGTTTGCAGATCGGAAGAGCACACGTCTG263270.14078988327867856Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAACT259280.1386561360447289Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTGCGATCCGTTGAAACTCATCTCAGCAGATCGGAAGAGCACACGTCTG257490.1376988910450372Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTCTCGCGTCAAGATATGCCTAGGTGGGAGATCGGAAGAGCACACGTC248900.13310518459400272No Hit
CTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAACTCC248270.13276827713601067Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGACCTCAGATTAGTCGAGAGCACTCGCTAGATCGGAAGAGCACACGTCT241760.1292869000700928Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATTGCGATCCGTTGAAACTCATCTCAGCGAGATCGGAAGAGCACACGTC240090.12839382791954243No Hit
CTCCGGCGACAATGAAATACCACTACAGATCGGAAGAGCACACGTCTGAA233080.12464506398220229Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGAATCAACTGTAGACGACTTTAAGCAGATCGGAAGAGCACACGTCTGA230340.1231797839268083Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTTGGGCCAACGTCTATCTGAGAGATCGGAAGAGCACACGTCTGAACT229670.12282148551910246Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCCGGCGACAATGAAATACCACAGATCGGAAGAGCACACGTCTGAACTC228390.12213697512826148Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAACT223920.11974653649774644Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGATCGGAAGAGCACACGT221150.1182652132300671No Hit
TTAAACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGA219070.11715288384495047Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGCGCTATGTGGCATATAGCTCGAGAGATCGGAAGAGCACACGTCTGAA217830.11648976440382328Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCTTGCGGATGGAGCAGACGAGAGATCGGAAGAGCACACGTCTGAACTC217050.11607264088440454Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCTGGTAGAGATAAATCGATGCAGATCGGAAGAGCACACGTCTGAACTC215440.11521165515842484Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGAAAAGCTCAAAGCCGAATCGTTTTAGATCGGAAGAGCACACGTCTGAA208300.11139337063451493Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAGTCTGAATATTCGTGCATGGAATAATGAAAGATCGGAAGAGCACACG204260.10923288471342305No Hit
GTGCGAGTTTAAGTACATTTGAATGTGTCTAGATCGGAAGAGCACACGTC201700.10786386393174105No Hit
GACTTGGGCCAACGTCTATCTGAAGATCGGAAGAGCACACGTCTGAACTC196830.10525951580408821Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCATTGATGCCATGCTTCCAGGAAAAGCCTCAGATCGGAAGAGCACACGT195330.10445735518982142No Hit
GTGTGAGTCCCATCTGACGAGCGCGGCGAGAGATCGGAAGAGCACACGTC192960.1031899414192799No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)