FastQCFastQC Report
Thu 28 Sept 2023
SRR3199155.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3199155.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13142157
Total Bases670.2 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACCTCAGATTAGTCGAGAGCACTCGCTGAGATCGGAAGAGCACACGTC1427881.0864883139046353No Hit
GACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGT1216190.9254112547886926No Hit
GGAGCTCCGGCGACAATGAAATACCACTACAGATCGGAAGAGCACACGTC949170.7222330398274803No Hit
GGAGCTCCGGCGACAATGAAATACCACAGATCGGAAGAGCACACGTCTGA942600.7172338604690235Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGATCGGAAGAGCACACGTCT907730.6907009252742909Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGGTGGGAGCTCCGGCGACAATGAAATACCACAGATCGGAAGAGCACAC838780.6382361738640011No Hit
GGTGACCGGTTGGGAATACCTCGTGCGCTAAGCAAGATCGGAAGAGCACA753190.5731098783860215No Hit
TTGTGACATCCGGCGCGCTTGTGGCGAGATCGGAAGAGCACACGTCTGAA717130.5456714601720251Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG615890.46863692162557485TruSeq Adapter, Index 5 (100% over 49bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGAGATCGGAAGAGCACACGT564830.42978485190825216No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAAGATCGGAAGAGCACACGTC558500.42496829097384853No Hit
GAGTGATGGCCGCATCGGCGACTCAGGAGATCGGAAGAGCACACGTCTGA521990.3971874632147524Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAGCTCCGGCGACAATGAAATACCACTACTAGATCGGAAGAGCACACGT478610.36417918306713276No Hit
AGGTGGGAGCTCCGGCGACAATGAAATACCACAGATCGGAAGAGCACACG464020.3530775047048974No Hit
GGGAGCTCCGGCGACAATGAAATACCACTACAGATCGGAAGAGCACACGT458970.3492349086987775No Hit
TAAACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAA445150.3387191311137129Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGTAGATCGGAAGAGCACACG434990.3309882844954599No Hit
GGAGCTCCGGCGACAATGAAATACCAGATCGGAAGAGCACACGTCTGAAC403860.3073011530755568Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGTGACATCCGGCGCGCTTGTGGCAGATCGGAAGAGCACACGTCTGAAC399530.3040064123415966Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGTAAACGATGTCTACTTGGAGGTTGTGGCAGATCGGAAGAGCACACGT366350.27875941521623887No Hit
GCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTGAA357490.27201775172827414Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGA357320.2718883970112364Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGAGCTCCGGCGACAATGAAATACCACTACTAGATCGGAAGAGCACACG355330.2703741859117952No Hit
ATTGCGATCCGTTGAAACTCATCTCAGCGAGATCGGAAGAGCACACGTCT353860.26925564806446917Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGGTGGGAGCTCCGGCGACAATGAAATACCACTACAGATCGGAAGAGCA330630.2515797064363179No Hit
CGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTGA328460.24992853151883668Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCATTGATGCCATGCTTCCAGGAAAAGCCTAGATCGGAAGAGCACACGTC325580.24773711043019803No Hit
GCTTAGCGTCATACCGCGTAGAATAAACAGATCGGAAGAGCACACGTCTG320440.24382603251505822Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGAGCTCCGGCGACAATGAAATACCACTACTAGATCGGAAGAGCACA316110.24053129178109803No Hit
GGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAAC304090.2313851523764326Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGAGCTCCGGCGACAATGAAATACCACAGATCGGAAGAGCACACGTCTG295820.22509242584759867Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTGATGCCATGCTTCCAGGAAAAGCCTAGATCGGAAGAGCACACGTCTG285910.2175518067544011Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAGCTCCGGCGACAATGAAATAGATCGGAAGAGCACACGTCTGAACTCC272180.20710451107835648Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGGGAGCTCCGGCGACAATGAAATACCACTACAGATCGGAAGAGCACAC270220.20561312728192183No Hit
AAACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAAC267310.20339887889027652Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTAGAACATCGAAACTCTGAACGCAAATTGAGATCGGAAGAGCACACGTC253870.1931722471432962No Hit
GGAAATTGCCTTGATGTGGGGGATAACCAAGATCGGAAGAGCACACGTCT245690.18694800252348226Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGAAC238490.18146944980188565Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGCAGAGCGCTATGTGGCATATAGCTCGAGAGATCGGAAGAGCACACGT237070.18038895745957076No Hit
CAAGTGATCCCTGGAATCGGTTTGCCCGAGATCGGAAGAGCACACGTCTG220750.16797090462395176Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTG220270.16760566777584532Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACG220200.16755240406882982No Hit
GGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAA216040.1643870180519073Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTAAACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTG212520.16170861449912674Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTTGGGCCAACGTCTATCTGAGGGTAGATCGGAAGAGCACACGTCTGA209740.15959328442051027Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGTAAACGATGTCTACTTGGAGGTTGTGGCAGATCGGAAGAGCACACG207710.15804863691706011No Hit
CGTTAAACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCT207620.15798015500804016Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGTGGTGAGTCTGGATAAACTAGCTGATCAGATCGGAAGAGCACACGTC207300.1577366637759692No Hit
AGCTCCGGCGACAATGAAATACCACTACAGATCGGAAGAGCACACGTCTG200210.152341811165397Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGAGCTCCGGCGACAATGAAATACCACAGATCGGAAGAGCACACGTC197220.15006668996573394No Hit
TGTGACATCCGGCGCGCTTGTGGCGAGATCGGAAGAGCACACGTCTGAAC191880.14600343003054977Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTCCGGCGACAATGAAATACCACTACTAGATCGGAAGAGCACACGTCT187930.14299783513467385Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTGCGCATAACCGCCCGATGCGAGAGCAGATCGGAAGAGCACACGTCTG187800.14289891682164504Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTGCGATCCGTTGAAACTCATCTCAGCAGATCGGAAGAGCACACGTCTG185940.14148362403523257Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGAGCGCAACCCTTATCCTTGTTTGCAGATCGGAAGAGCACACGTCTG185920.14146840583322814Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTCTCGCGTCAAGATATGCCTAGGTGGGAGATCGGAAGAGCACACGTC185560.1411944781971483No Hit
GGAGCTCCGGCGACAATGAAATACCAAGATCGGAAGAGCACACGTCTGAA183200.13939873036062497Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTTAGCGTCATACCGCGTAGAATAGATCGGAAGAGCACACGTCTGAACT181000.13772472814013711Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAACTCC178970.13618008063668696Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGACCTCAGATTAGTCGAGAGCACTCGCTAGATCGGAAGAGCACACGTCT176210.13407996876007494Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATTGCGATCCGTTGAAACTCATCTCAGCGAGATCGGAAGAGCACACGTC176010.13392778674003056No Hit
GCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAACT174810.13301469461976448Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGGTGGGAGCTCCGGCGACAGATCGGAAGAGCACACGTCTGAACTCCAG165640.126037149000731Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTAAACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGA164850.12543603002155582Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCGGCGACAATGAAATACCACTACAGATCGGAAGAGCACACGTCTGAA162040.12329787263993269Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGCGCTATGTGGCATATAGCTCGAGAGATCGGAAGAGCACACGTCTGAA158620.1206955600971743Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCCGGCGACAATGAAATACCACAGATCGGAAGAGCACACGTCTGAACTC156130.11880089394762214Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGAATCAACTGTAGACGACTTTAAGCAGATCGGAAGAGCACACGTCTGA155490.11831391148348022Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGGTAGAGATAAATCGATGCAGATCGGAAGAGCACACGTCTGAACTC154830.11781171081733385Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GACTTGGGCCAACGTCTATCTGAGAGATCGGAAGAGCACACGTCTGAACT153540.11683013678804781Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAACT152070.11571159894072183Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGATCGGAAGAGCACACGT149910.11406803312424285No Hit
GGAAAAGCTCAAAGCCGAATCGTTTTAGATCGGAAGAGCACACGTCTGAA142630.10852860759462848Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCTTGCGGATGGAGCAGACGAGAGATCGGAAGAGCACACGTCTGAACTC141620.10776008839340452Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGTCTGAATATTCGTGCATGGAATAATGAAAGATCGGAAGAGCACACG141110.10737202424229142No Hit
AAAAGCTCAAAGCCGAATCGTTTTAGATCGGAAGAGCACACGTCTGAACT137350.10451100226545765Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGCGAGTTTAAGTACATTTGAATGTGTCTAGATCGGAAGAGCACACGTC137040.1042751201343889No Hit
TCATTGATGCCATGCTTCCAGGAAAAGCCTCAGATCGGAAGAGCACACGT136850.10413054721534676No Hit
AGCTGGTAGAGATAAATCGATGCCAGATCGGAAGAGCACACGTCTGAACT132000.10044013322927127Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)