FastQCFastQC Report
Thu 28 Sept 2023
SRR3199156.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3199156.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13231977
Total Bases674.8 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACCTCAGATTAGTCGAGAGCACTCGCTGAGATCGGAAGAGCACACGTC1452031.0973643621055267No Hit
GACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGT1264970.9559947088783484No Hit
GGAGCTCCGGCGACAATGAAATACCACTACAGATCGGAAGAGCACACGTC988840.7473108515832517No Hit
GGAGCTCCGGCGACAATGAAATACCACAGATCGGAAGAGCACACGTCTGA976280.7378186948178643Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGATCGGAAGAGCACACGTCT950350.7182222278651179Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGGTGGGAGCTCCGGCGACAATGAAATACCACAGATCGGAAGAGCACAC863660.6527066968148448No Hit
GGTGACCGGTTGGGAATACCTCGTGCGCTAAGCAAGATCGGAAGAGCACA774210.5851053096600757No Hit
TTGTGACATCCGGCGCGCTTGTGGCGAGATCGGAAGAGCACACGTCTGAA728570.5506131094393528Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG614170.4641558853979265TruSeq Adapter, Index 5 (100% over 49bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGAGATCGGAAGAGCACACGT588240.44455941844518015No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAAGATCGGAAGAGCACACGTC580880.4389971354998577No Hit
GAGTGATGGCCGCATCGGCGACTCAGGAGATCGGAAGAGCACACGTCTGA543000.4103695162106161Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAGCTCCGGCGACAATGAAATACCACTACTAGATCGGAAGAGCACACGT499760.3776911039068463No Hit
GGGAGCTCCGGCGACAATGAAATACCACTACAGATCGGAAGAGCACACGT481020.3635284432553049No Hit
AGGTGGGAGCTCCGGCGACAATGAAATACCACAGATCGGAAGAGCACACG471950.3566738364191534No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAGTAGATCGGAAGAGCACACG456670.34512605334788593No Hit
TAAACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAA445450.3366465948361307Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGAGCTCCGGCGACAATGAAATACCAGATCGGAAGAGCACACGTCTGAAC418960.31662691070276194Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGTGACATCCGGCGCGCTTGTGGCAGATCGGAAGAGCACACGTCTGAAC408150.30845730762681944Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGAGCTCCGGCGACAATGAAATACCACTACTAGATCGGAAGAGCACACG372860.2817870677979564No Hit
CCGTAAACGATGTCTACTTGGAGGTTGTGGCAGATCGGAAGAGCACACGT371410.28069123759813064No Hit
GCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTGAA370540.2800337394782352Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGA369240.27905127102321897Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTGCGATCCGTTGAAACTCATCTCAGCGAGATCGGAAGAGCACACGTCT354760.2681080839242692Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGGTGGGAGCTCCGGCGACAATGAAATACCACTACAGATCGGAAGAGCA341150.2578223949452149No Hit
TCATTGATGCCATGCTTCCAGGAAAAGCCTAGATCGGAAGAGCACACGTC333940.2523734737447019No Hit
GCTTAGCGTCATACCGCGTAGAATAAACAGATCGGAAGAGCACACGTCTG333430.25198804381234946Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTGA329760.24921445978934215Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGAGCTCCGGCGACAATGAAATACCACTACTAGATCGGAAGAGCACA329610.24910109804453257No Hit
GGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAAC317000.23957115403087534Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGAGCTCCGGCGACAATGAAATACCACAGATCGGAAGAGCACACGTCTG306570.23168873404178378Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTGATGCCATGCTTCCAGGAAAAGCCTAGATCGGAAGAGCACACGTCTG289890.2190829080189604Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGAGCTCCGGCGACAATGAAATACCACTACAGATCGGAAGAGCACAC284770.21521349379612736No Hit
GGAGCTCCGGCGACAATGAAATAGATCGGAAGAGCACACGTCTGAACTCC276550.20900127018056336Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAAC269680.2038093022682854Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTAGAACATCGAAACTCTGAACGCAAATTGAGATCGGAAGAGCACACGTC260150.19660705274805118No Hit
GGAAATTGCCTTGATGTGGGGGATAACCAAGATCGGAAGAGCACACGTCT253400.19150577423162088Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGCAGAGCGCTATGTGGCATATAGCTCGAGAGATCGGAAGAGCACACGT248600.18787819839771486No Hit
AAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGAAC238670.18037365089132185Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAA226900.17147853264859816Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTG224530.16968741708060708Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTAAACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTG223810.16914328070552118Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAAGTGATCCCTGGAATCGGTTTGCCCGAGATCGGAAGAGCACACGTCTG222790.16837242084081616Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACG222510.16816081225050497No Hit
GACTTGGGCCAACGTCTATCTGAGGGTAGATCGGAAGAGCACACGTCTGA221820.16763934822438098Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGTAAACGATGTCTACTTGGAGGTTGTGGCAGATCGGAAGAGCACACG215550.16290082729134128No Hit
TTGTGGTGAGTCTGGATAAACTAGCTGATCAGATCGGAAGAGCACACGTC212410.16052778809999443No Hit
CGTTAAACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCT212050.16025571991245147Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTCCGGCGACAATGAAATACCACTACAGATCGGAAGAGCACACGTCTG202260.15285697670121404Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGAGCTCCGGCGACAATGAAATACCACAGATCGGAAGAGCACACGTC201010.1519122954944677No Hit
TGTGACATCCGGCGCGCTTGTGGCGAGATCGGAAGAGCACACGTCTGAAC197060.1489271028811492Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTGCGCATAACCGCCCGATGCGAGAGCAGATCGGAAGAGCACACGTCTG195510.14775569818478373Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAGCTCCGGCGACAATGAAATACCAAGATCGGAAGAGCACACGTCTGAA193400.14616107630779587Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTCCGGCGACAATGAAATACCACTACTAGATCGGAAGAGCACACGTCT192150.14521639510104953Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTAGCGTCATACCGCGTAGAATAGATCGGAAGAGCACACGTCTGAACT189000.1428357984600487Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTGCGATCCGTTGAAACTCATCTCAGCAGATCGGAAGAGCACACGTCTG188470.14243525362838827Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGAGCGCAACCCTTATCCTTGTTTGCAGATCGGAAGAGCACACGTCTG188460.1424276961787343Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTCTCGCGTCAAGATATGCCTAGGTGGGAGATCGGAAGAGCACACGTC186400.1408708615500163No Hit
GCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAACT180960.13675960893825617Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CATTGCGATCCGTTGAAACTCATCTCAGCGAGATCGGAAGAGCACACGTC179300.135505072295697No Hit
CTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAACTCC178220.13468886773306815Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGACCTCAGATTAGTCGAGAGCACTCGCTAGATCGGAAGAGCACACGTCT177850.13440924209587124Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTAAACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGA168450.12730523942113867Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGGTGGGAGCTCCGGCGACAGATCGGAAGAGCACACGTCTGAACTCCAG166260.12565015794691906Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCCGGCGACAATGAAATACCACTACAGATCGGAAGAGCACACGTCTGAA162260.1226271780853307Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGCGCTATGTGGCATATAGCTCGAGAGATCGGAAGAGCACACGTCTGAA161800.12227953540124806Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCCGGCGACAATGAAATACCACAGATCGGAAGAGCACACGTCTGAACTC158670.11991405365955518Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGAATCAACTGTAGACGACTTTAAGCAGATCGGAAGAGCACACGTCTGA158010.1194152619823931Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTTGGGCCAACGTCTATCTGAGAGATCGGAAGAGCACACGTCTGAACT157880.1193170151368915Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAACT156420.11821362748741175Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCTGGTAGAGATAAATCGATGCAGATCGGAAGAGCACACGTCTGAACTC155640.11762414641440203Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGATCGGAAGAGCACACGT153210.1157876861484871No Hit
GGAAAAGCTCAAAGCCGAATCGTTTTAGATCGGAAGAGCACACGTCTGAA149180.11274203393793686Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAGTCTGAATATTCGTGCATGGAATAATGAAAGATCGGAAGAGCACACG143770.10865345367513865No Hit
GTGCGAGTTTAAGTACATTTGAATGTGTCTAGATCGGAAGAGCACACGTC142410.1076256405221986No Hit
CCCTTGCGGATGGAGCAGACGAGAGATCGGAAGAGCACACGTCTGAACTC142010.10732334253603978Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAAAGCTCAAAGCCGAATCGTTTTAGATCGGAAGAGCACACGTCTGAACT135620.10249413220715241Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCATTGATGCCATGCTTCCAGGAAAAGCCTCAGATCGGAAGAGCACACGT134950.10198778308033637No Hit
GACTTGGGCCAACGTCTATCTGAAGATCGGAAGAGCACACGTCTGAACTC134170.10139830200732664Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTGCGCATAACCGCCCGATGCGAGAGATCGGAAGAGCACACGTCTGAAC133870.10117157851770751Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)