FastQCFastQC Report
Thu 28 Sept 2023
SRR3199157.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3199157.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18764132
Total Bases956.9 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG4232122.255430733486633TruSeq Adapter, Index 7 (100% over 49bp)
GACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGT3834362.043451836727646No Hit
TGACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACG3255121.7347565024590534No Hit
TTGTGACATCCGGCGCGCTTGTGGCGAGATCGGAAGAGCACACGTCTGAA1705190.9087497359323629Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGTGACATCCGGCGCGCTTGTGGCAGATCGGAAGAGCACACGTCTGAAC1225300.6530011620041897Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGATGGCCGCATCGGCGACTCAGGAGATCGGAAGAGCACACGTCTGAA832540.4436869235411475Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGCGATCCGTTGAAACTCATCTCAGCGAGATCGGAAGAGCACACGTCT749540.39945359582846685Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTG653520.3482814979131462Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGAAC633100.3373990334325084Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGTGATGGCCGCATCGGCGACTCAGGAAGATCGGAAGAGCACACGTCTG632730.3372018487186085Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGACCTCAGATTAGTCGAGAGCACTCGCTGAGATCGGAAGAGCACACGTC613630.3270228540281No Hit
GGTGACCGGTTGGGAATACCTCGTGCGCTAAGCAAGATCGGAAGAGCACA610430.32531747271869543No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAGATCGGAAGAGCACACGTCT610110.32514693458775495Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGTGGTGAGTCTGGATAAACTAGCTGATCAGATCGGAAGAGCACACGTC604520.3221678466128889No Hit
GCACGCGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACG564630.30090920272784266No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAGTAGATCGGAAGAGCACACG542080.2888915938131324No Hit
TTGTGGTGAGTCTGGATAAACTAGCTGATCGCATAGATCGGAAGAGCACA536230.2857739436068772No Hit
AGCTGGTAGAGATAAATCGATGCAGATCGGAAGAGCACACGTCTGAACTC529220.28203809267596286Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTGAA504390.26880539957830185Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGA491260.26180800689315126Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTTGGGCCAACGTCTATCTGAGGGTAGATCGGAAGAGCACACGTCTGA489390.260811424690468Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTGA483370.25760317610215067Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGGTAGAGATAAATCGATGCCAAAGCAGATCGGAAGAGCACACGTCT471120.2510747632770863Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTTCGGGGGAGTGTTATAGTCCAGCCAGATCGGAAGAGCACACGTCTG456870.24348048713364415Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGAGATCGGAAGAGCACACGT446100.2377408131641794No Hit
TGTGACATCCGGCGCGCTTGTGGCGAGATCGGAAGAGCACACGTCTGAAC427790.22798283448443019Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCCGAGTGTCGTAAACGGTAGAGCAATTTAGATCGGAAGAGCACACGTC410370.2186991649813591No Hit
GAGTGATGGCCGCATCGGCGACTCAGGAGATCGGAAGAGCACACGTCTGA409480.21822485580468096Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTTGGGCCAACGTCTATCTGAGAGATCGGAAGAGCACACGTCTGAACT399410.2128582339966485Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAACT397600.21189362769351655Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGATAGGGATAGCGATCACGTAAAGTGTCACAAGATCGGAAGAGCACAC397220.21169111366302473No Hit
TGACTTGGGCCAACGTCTATCTGAGGGTAGATCGGAAGAGCACACGTCTG396910.2115259048486762Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGCAGAGCGCTATGTGGCATATAGCTCGAGAGATCGGAAGAGCACACGT396340.2112221338029385No Hit
TGTGACATCCGGCGCGCTTGTGGCAGATCGGAAGAGCACACGTCTGAACT375600.2001691311913602Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGAGCTGGTAGAGATAAATCGATGCCAGATCGGAAGAGCACACGTCTGA368810.1965505252254674Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTGGAGCAGAGCGCTATGTGGCATATAGCTCGAGAGATCGGAAGAGC366480.1953087944595572No Hit
AGCTGGTAGAGATAAATCGATGCCAGATCGGAAGAGCACACGTCTGAACT365780.19493574229812494Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCCGAGTGTCGTAAACGGTAGAGCAGATCGGAAGAGCACACGTCTGAAC364650.19433352952324148Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCT363180.19355011998423374Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGCGATCCGTTGAAACTCATCTCAGCGATAGATCGGAAGAGCACACGT356280.18987289153583017No Hit
GACTTGGGCCAACGTCTATCTGAGGGTCAGATCGGAAGAGCACACGTCTG352290.18774649421566636Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CATAGAAGACGATCAAACTCAGTCAGATCGGAAGAGCACACGTCTGAACT351290.18721356255647742Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGCTGGTAGAGATAAATCGATGCCAAAGCAGATCGGAAGAGCACACGTC347750.18532698448294863No Hit
TTGTGACATCCGGCGCGCTTGTGGCGGCAGATCGGAAGAGCACACGTCTG341670.18208675999507998Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAGTCTGAATATTCGTGCATGGAATAATGAAAAGATCGGAAGAGCACAC339430.1808929930784968No Hit
TTGTGGTGAGTCTGGATAAACTAGCTGATCGCAAGATCGGAAGAGCACAC331200.17650696552337192No Hit
ACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTC326160.17382098996105974No Hit
TCCGTGTTAGTATAGTGGTGAGTATTCCCAGATCGGAAGAGCACACGTCT324960.17318147197003306Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAAGTGATCCCTGGAATCGGTTTGCCCGAGATCGGAAGAGCACACGTCTG319930.17050082572431274Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAAC317960.16945095035571056Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGCGAGTTTAAGTACATTTGAATGTGTCTAGATCGGAAGAGCACACGTC316340.1685876010678245No Hit
TGGAGCTGGTAGAGATAAATCGATGCAGATCGGAAGAGCACACGTCTGAA311760.16614677406873923Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAAGATCGGAAGAGCACACGTC310630.16554456129385575No Hit
ACGCGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTC304070.1620485296095764No Hit
ATTGCGATCCGTTGAAACTCATCTCAGCAGATCGGAAGAGCACACGTCTG303570.16178206377998194Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGCTGGTAGAGATAAATCGATGCAGATCGGAAGAGCACACGTCTGAACT300510.16015129290286384Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CATTGCGATCCGTTGAAACTCATCTCAGCGAGATCGGAAGAGCACACGTC294400.1568950804652195No Hit
TTTTTGGAGCAGAGCGCTATGTGGCATAGATCGGAAGAGCACACGTCTGA291050.15510975940693658Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGTGGTGAGTCTGGATAAACTAGCTGATCGCAGATCGGAAGAGCACACG282350.15047325397199296No Hit
GACTTGGGCCAACGTCTATCTGAAGATCGGAAGAGCACACGTCTGAACTC281770.15016415360966337Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGTGATGGCCGCATCGGCGACTCAGGAGAGATCGGAAGAGCACACGTCT277300.1477819490930889Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCGAAAGGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGA273960.14600195735139787No Hit
ATCCGAGTGTCGTAAACGGTAGAGCAATTAGATCGGAAGAGCACACGTCT270330.14406741542854207Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGACTTGGGCCAACGTCTATCTGAGGGTCAGATCGGAAGAGCACACGTCT270140.14396615841329616Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGAATCAACTGTAGACGACTTTAAGCAGATCGGAAGAGCACACGTCTGA255380.13610008712366764Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGACTTGGGCCAACGTCTATCTGAGAGATCGGAAGAGCACACGTCTGAAC253810.13526338441874103Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTCGGGGGAGTGTTATAGTCCAGCCGAGATCGGAAGAGCACACGTCTGA253470.1350821876546168Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTAGCGTCATACCGCGTAGAATAAACAGATCGGAAGAGCACACGTCTG251280.13391506732099306Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGCTGGTAGAGATAAATCGATGCCAGATCGGAAGAGCACACGTCTGAAC234460.12495115681343534Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGCTGGTAGAGATAAATCGATGCCAAAGAGATCGGAAGAGCACACGTCT234000.12470600825020843Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAACT227390.12118332998296963Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACTTGGGCCAACGTCTATCTGAGGAGATCGGAAGAGCACACGTCTGAAC225460.120154771880735Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTCGGGGGAGTGTTATAGTCCAGCCAGATCGGAAGAGCACACGTCTGAA224100.11942998482423807Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAA223880.11931273985921652Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTATACACTGACGAGTAGGAGGGCGTGTCGAGATCGGAAGAGCACACGT222240.11843873193814666No Hit
GCGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTG219420.1169358646592339Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTGCGCATAACCGCCCGATGCGAGAGCAGATCGGAAGAGCACACGTCTG219040.1167333506287421Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTTCGGGGGAGTGTTATAGTCCAGCCGAGATCGGAAGAGCACACGTCT216460.11535838694803469Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGAGTGTTATAGTCCAGCCGAGATCGGAAGAGCACACGTCTGAACTC212660.11333324664311677Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGCGATGGATCATTCAAGTGTCTGACCAGATCGGAAGAGCACACGTCTG210330.11209151587720657Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGACTTGGGCCAACGTCTATCTGAAGATCGGAAGAGCACACGTCTGAACT209340.11156391353460954Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGTGATGGCCGCATCGGCGACTCAGGAGGAGATCGGAAGAGCACACGTC208750.11124948385568807No Hit
ATTGCGATCCGTTGAAACTCATCTCAGCGAAGATCGGAAGAGCACACGTC207640.11065792971398838No Hit
TTAGAACATCGAAACTCTGAACGAGATCGGAAGAGCACACGTCTGAACTC205420.10947482143058897Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGAA205370.1094481748476295Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGCGATCCGTTGAAACTCATCTCAGATCGGAAGAGCACACGTCTGAAC205110.10930961261624039Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAACTCC202480.10790800235257353Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTGGTGAGTCTGGATAAACTAGCTGATCGCATAGATCGGAAGAGCACAC201710.10749764497499803No Hit
GCAGCTAAATGCGAGAATTAGTGTGAATTGCAGGAGATCGGAAGAGCACA201510.10739105864316027No Hit
CTCCTTCGGGGGAGTGTTATAGTCCAGCCAGATCGGAAGAGCACACGTCT198670.10587753273106372Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGCGCTATGTGGCATATAGCTCGAGAGATCGGAAGAGCACACGTCTGA198410.10573897049967458Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGTGAGTCCCATCTGACGAGCGCGGCGAGCAGATCGGAAGAGCACACGT195070.10395897875798359No Hit
GGAAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGA192210.10243479421270325Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTAAGCTGTGTGAGAATGTGATGCTAGATCGGAAGAGCACACGTCTGAA191960.10230156129790602Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)