FastQCFastQC Report
Thu 28 Sept 2023
SRR3199158.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3199158.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18774138
Total Bases957.4 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG4201012.237657995269876TruSeq Adapter, Index 7 (100% over 49bp)
GACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGT3847172.049185959962583No Hit
TGACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACG3254291.7333898365932965No Hit
TTGTGACATCCGGCGCGCTTGTGGCGAGATCGGAAGAGCACACGTCTGAA1692890.9017138363423131Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGTGACATCCGGCGCGCTTGTGGCAGATCGGAAGAGCACACGTCTGAAC1222140.6509699673028929Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGATGGCCGCATCGGCGACTCAGGAGATCGGAAGAGCACACGTCTGAA829620.44189512189587615Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGCGATCCGTTGAAACTCATCTCAGCGAGATCGGAAGAGCACACGTCT743000.39575718469737464Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTG647430.34485205126328566Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGAAC637760.33970134873835484Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGTGATGGCCGCATCGGCGACTCAGGAAGATCGGAAGAGCACACGTCTG632210.3367451544246665Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGATCGGAAGAGCACACGTCT615190.32767949186268897Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGACCTCAGATTAGTCGAGAGCACTCGCTGAGATCGGAAGAGCACACGTC613370.32671007318684886No Hit
GGTGACCGGTTGGGAATACCTCGTGCGCTAAGCAAGATCGGAAGAGCACA612730.32636917870743254No Hit
TTGTGGTGAGTCTGGATAAACTAGCTGATCAGATCGGAAGAGCACACGTC601140.3201957927442528No Hit
GCACGCGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACG567640.30235209733730517No Hit
TTGTGGTGAGTCTGGATAAACTAGCTGATCGCATAGATCGGAAGAGCACA538170.2866549718554322No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAGTAGATCGGAAGAGCACACG538050.28659105414054165No Hit
AGCTGGTAGAGATAAATCGATGCAGATCGGAAGAGCACACGTCTGAACTC525870.28010340607914996Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTGAA505430.2692160886427915Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTTGGGCCAACGTCTATCTGAGGGTAGATCGGAAGAGCACACGTCTGA491540.2618176131442093Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGA490460.26124235371019433Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTGA483250.25740196434051993Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGGTAGAGATAAATCGATGCCAAAGCAGATCGGAAGAGCACACGTCT466550.24850674901825054Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTTCGGGGGAGTGTTATAGTCCAGCCAGATCGGAAGAGCACACGTCTG458900.24443199469397744Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGAGATCGGAAGAGCACACGT449430.2393878216938642No Hit
TGTGACATCCGGCGCGCTTGTGGCGAGATCGGAAGAGCACACGTCTGAAC421060.224276608598488Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCCGAGTGTCGTAAACGGTAGAGCAATTTAGATCGGAAGAGCACACGTC408140.2173948012952712No Hit
GAGTGATGGCCGCATCGGCGACTCAGGAGATCGGAAGAGCACACGTCTGA407580.21709651862578191Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAACT403900.21513637536913813Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGCAGAGCGCTATGTGGCATATAGCTCGAGAGATCGGAAGAGCACACGT400420.2132827616373119No Hit
GACTTGGGCCAACGTCTATCTGAGAGATCGGAAGAGCACACGTCTGAACT400320.21322949687490314Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGATAGGGATAGCGATCACGTAAAGTGTCACAAGATCGGAAGAGCACAC399450.21276609344194658No Hit
TGACTTGGGCCAACGTCTATCTGAGGGTAGATCGGAAGAGCACACGTCTG396520.21120543590336877Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTGACATCCGGCGCGCTTGTGGCAGATCGGAAGAGCACACGTCTGAACT375830.2001849565609883Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTTTGGAGCAGAGCGCTATGTGGCATATAGCTCGAGAGATCGGAAGAGC374840.19965763541314122No Hit
TGGAGCTGGTAGAGATAAATCGATGCCAGATCGGAAGAGCACACGTCTGA368770.19642446433492713Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGGTAGAGATAAATCGATGCCAGATCGGAAGAGCACACGTCTGAACT368180.19611020223671521Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCCGAGTGTCGTAAACGGTAGAGCAGATCGGAAGAGCACACGTCTGAAC364400.19409679421766263Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCT360000.19175314467167545Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGCGATCCGTTGAAACTCATCTCAGCGATAGATCGGAAGAGCACACGT358710.19106602923660196No Hit
CATAGAAGACGATCAAACTCAGTCAGATCGGAAGAGCACACGTCTGAACT356730.19001138694090774Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACTTGGGCCAACGTCTATCTGAGGGTCAGATCGGAAGAGCACACGTCTG351780.18737478120167222Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGCTGGTAGAGATAAATCGATGCCAAAGCAGATCGGAAGAGCACACGTC347780.18524419070532028No Hit
TTGTGACATCCGGCGCGCTTGTGGCGGCAGATCGGAAGAGCACACGTCTG343550.18299109125542806Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAGTCTGAATATTCGTGCATGGAATAATGAAAAGATCGGAAGAGCACAC342290.1823199552490772No Hit
TTGTGGTGAGTCTGGATAAACTAGCTGATCGCAAGATCGGAAGAGCACAC330930.1762690782394377No Hit
TCCGTGTTAGTATAGTGGTGAGTATTCCCAGATCGGAAGAGCACACGTCT327380.1743781791739253Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTC326380.17384553154983734No Hit
CAAGTGATCCCTGGAATCGGTTTGCCCGAGATCGGAAGAGCACACGTCTG320220.17056442218545534Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAAC315490.16804499892351915Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGCGAGTTTAAGTACATTTGAATGTGTCTAGATCGGAAGAGCACACGTC315460.1680290194947965No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAAGATCGGAAGAGCACACGTC315370.1679810812086286No Hit
TGGAGCTGGTAGAGATAAATCGATGCAGATCGGAAGAGCACACGTCTGAA306870.1634535764038807Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGCGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTC303670.16174910400679915No Hit
ATTGCGATCCGTTGAAACTCATCTCAGCAGATCGGAAGAGCACACGTCTG303420.16161594210077715Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGCTGGTAGAGATAAATCGATGCAGATCGGAAGAGCACACGTCTGAACT298510.15900064226650512Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CATTGCGATCCGTTGAAACTCATCTCAGCGAGATCGGAAGAGCACACGTC297380.1583987504512857No Hit
TTTTTGGAGCAGAGCGCTATGTGGCATAGATCGGAAGAGCACACGTCTGA290290.15462227879650187Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTTGGGCCAACGTCTATCTGAAGATCGGAAGAGCACACGTCTGAACTC283340.15092037780909034Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGTGGTGAGTCTGGATAAACTAGCTGATCGCAGATCGGAAGAGCACACG281560.14997226503821376No Hit
TGACTTGGGCCAACGTCTATCTGAGGGTCAGATCGGAAGAGCACACGTCT277210.14765524787343098Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCGAAAGGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGA275500.14674442043624053No Hit
GAGTGATGGCCGCATCGGCGACTCAGGAGAGATCGGAAGAGCACACGTCT275240.14660593205397765Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCGAGTGTCGTAAACGGTAGAGCAATTAGATCGGAAGAGCACACGTCT274780.14636091414689717Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTAGCGTCATACCGCGTAGAATAAACAGATCGGAAGAGCACACGTCTG255360.13601689728710847Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGACTTGGGCCAACGTCTATCTGAGAGATCGGAAGAGCACACGTCTGAAC254980.13581449118995503Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTCGGGGGAGTGTTATAGTCCAGCCGAGATCGGAAGAGCACACGTCTGA251990.13422187479393194Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGAATCAACTGTAGACGACTTTAAGCAGATCGGAAGAGCACACGTCTGA251240.13382238907586597Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGCTGGTAGAGATAAATCGATGCCAAAGAGATCGGAAGAGCACACGTCT237260.12637597529111588Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGCTGGTAGAGATAAATCGATGCCAGATCGGAAGAGCACACGTCTGAAC235640.12551308614009335Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTCGGGGGAGTGTTATAGTCCAGCCAGATCGGAAGAGCACACGTCTGAA225480.1201013862793594Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAACT225220.11996289789709652Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACTTGGGCCAACGTCTATCTGAGGAGATCGGAAGAGCACACGTCTGAAC224730.1197019005612934Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAA224430.11954210627406701Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTATACACTGACGAGTAGGAGGGCGTGTCGAGATCGGAAGAGCACACGT222020.11825842550001496No Hit
GCTGCGCATAACCGCCCGATGCGAGAGCAGATCGGAAGAGCACACGTCTG221090.11776306320961313Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTG220250.11731563920537923Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTTCGGGGGAGTGTTATAGTCCAGCCGAGATCGGAAGAGCACACGTCT217350.11577096109552407Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGAGTGTTATAGTCCAGCCGAGATCGGAAGAGCACACGTCTGAACTC213620.11378418545767587Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGCGATGGATCATTCAAGTGTCTGACCAGATCGGAAGAGCACACGTCTG212530.11320359954741996Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGAA210060.11188795991592262Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGTGATGGCCGCATCGGCGACTCAGGAGGAGATCGGAAGAGCACACGTC209810.11175479800990065No Hit
ATTGCGATCCGTTGAAACTCATCTCAGCGAAGATCGGAAGAGCACACGTC209200.11142988295920696No Hit
ATTGCGATCCGTTGAAACTCATCTCAGATCGGAAGAGCACACGTCTGAAC208510.11106235609858627Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGACTTGGGCCAACGTCTATCTGAAGATCGGAAGAGCACACGTCTGAACT208070.11082799114398754Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTAGAACATCGAAACTCTGAACGAGATCGGAAGAGCACACGTCTGAACTC205200.10929929246285502Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCAGCTAAATGCGAGAATTAGTGTGAATTGCAGGAGATCGGAAGAGCACA204570.10896372445967958No Hit
CTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAACTCC200080.10657213662752453Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTGGTGAGTCTGGATAAACTAGCTGATCGCATAGATCGGAAGAGCACAC200020.10654017777007925No Hit
AGAGCGCTATGTGGCATATAGCTCGAGAGATCGGAAGAGCACACGTCTGA196780.10481439946803417Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCTTCGGGGGAGTGTTATAGTCCAGCCAGATCGGAAGAGCACACGTCT195660.10421783412905561Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGA192500.10253466763693758Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGTGAGTCCCATCTGACGAGCGCGGCGAGCAGATCGGAAGAGCACACGT192040.1022896497298571No Hit
CGTAAGCTGTGTGAGAATGTGATGCTAGATCGGAAGAGCACACGTCTGAA191900.10221507906248478Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGCGATGGATCATTCAAGTGTCTGACCTATCAGATCGGAAGAGCACACG188980.10065974800014786No Hit
CAAGTGATCCCTGGAATCGGTTTGCCCAGATCGGAAGAGCACACGTCTGA188940.10063844209518434Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)