FastQCFastQC Report
Thu 28 Sept 2023
SRR3199159.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3199159.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13303574
Total Bases678.4 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG2949572.2171260144078575TruSeq Adapter, Index 7 (100% over 49bp)
GACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGT2516991.8919652718885918No Hit
TGACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACG2180651.6391459918966136No Hit
TTGTGACATCCGGCGCGCTTGTGGCGAGATCGGAAGAGCACACGTCTGAA1077960.8102785011005312Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGTGACATCCGGCGCGCTTGTGGCAGATCGGAAGAGCACACGTCTGAAC733850.5516186853247105Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGATGGCCGCATCGGCGACTCAGGAGATCGGAAGAGCACACGTCTGAA578330.4347177683230085Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGCGATCCGTTGAAACTCATCTCAGCGAGATCGGAAGAGCACACGTCT522770.3929545549188511Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGAAC474000.35629523314561934Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTG439710.33052020457059134Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGACCTCAGATTAGTCGAGAGCACTCGCTGAGATCGGAAGAGCACACGTC432150.32483752110523084No Hit
GAGTGATGGCCGCATCGGCGACTCAGGAAGATCGGAAGAGCACACGTCTG414660.3116906780087817Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACGCGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACG413280.3106533627730413No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAGATCGGAAGAGCACACGTCT408760.3072557795371379Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGTGGTGAGTCTGGATAAACTAGCTGATCAGATCGGAAGAGCACACGTC403290.30314410247952916No Hit
AGCTGGTAGAGATAAATCGATGCAGATCGGAAGAGCACACGTCTGAACTC386570.29057605121751495Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGTGACCGGTTGGGAATACCTCGTGCGCTAAGCAAGATCGGAAGAGCACA361720.27189686019711695No Hit
TTGTGGTGAGTCTGGATAAACTAGCTGATCGCATAGATCGGAAGAGCACA358720.26964182707594214No Hit
GCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTGAA357220.26851431051535474Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGTAGATCGGAAGAGCACACG349690.262854177381206No Hit
CGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTGA348320.2618243789225361Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGGTAGAGATAAATCGATGCCAAAGCAGATCGGAAGAGCACACGTCT344880.2592386076102557Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGA322920.24273176516325612Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTTGGGCCAACGTCTATCTGAGGGTAGATCGGAAGAGCACACGTCTGA319770.24036398038602258Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTTCGGGGGAGTGTTATAGTCCAGCCAGATCGGAAGAGCACACGTCTG303690.22827700285652563Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGAGATCGGAAGAGCACACGT294700.2215194202700718No Hit
GAGCAGAGCGCTATGTGGCATATAGCTCGAGAGATCGGAAGAGCACACGT291350.2190012999514266No Hit
TGTGACATCCGGCGCGCTTGTGGCGAGATCGGAAGAGCACACGTCTGAAC280640.21095083170883253Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTGCGATCCGTTGAAACTCATCTCAGCGATAGATCGGAAGAGCACACGT274660.20645579902062405No Hit
GCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAACT272440.20478707451095474Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGTGATGGCCGCATCGGCGACTCAGGAGATCGGAAGAGCACACGTCTGA271860.20435110144086094Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTTGGGCCAACGTCTATCTGAGAGATCGGAAGAGCACACGTCTGAACT271260.20390009481662594Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCCGAGTGTCGTAAACGGTAGAGCAATTTAGATCGGAAGAGCACACGTC269470.20255459172099166No Hit
GAGATAGGGATAGCGATCACGTAAAGTGTCACAAGATCGGAAGAGCACAC262510.19732291487986614No Hit
AGCTGGTAGAGATAAATCGATGCCAGATCGGAAGAGCACACGTCTGAACT261780.1967741901537136Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGACTTGGGCCAACGTCTATCTGAGGGTAGATCGGAAGAGCACACGTCTG259770.19526331796252647Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTGGAGCAGAGCGCTATGTGGCATATAGCTCGAGAGATCGGAAGAGC256030.19245204333812854No Hit
TGGAGCTGGTAGAGATAAATCGATGCCAGATCGGAAGAGCACACGTCTGA255850.19231674135085805Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCT246830.18553660843319247Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATAGAAGACGATCAAACTCAGTCAGATCGGAAGAGCACACGTCTGAACT242850.18254493115910056Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTGACATCCGGCGCGCTTGTGGCAGATCGGAAGAGCACACGTCTGAACT235530.17704265034343403Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGTGGTGAGTCTGGATAAACTAGCTGATCGCAAGATCGGAAGAGCACAC235210.1768021134771754No Hit
ATCCGAGTGTCGTAAACGGTAGAGCAGATCGGAAGAGCACACGTCTGAAC234160.1760128518847642Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAGTCTGAATATTCGTGCATGGAATAATGAAAAGATCGGAAGAGCACAC233070.1751935231840707No Hit
GACTTGGGCCAACGTCTATCTGAGGGTCAGATCGGAAGAGCACACGTCTG231630.17411110728590679Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAAGTGATCCCTGGAATCGGTTTGCCCGAGATCGGAAGAGCACACGTCTG227030.17065338983343875Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGCTGGTAGAGATAAATCGATGCCAAAGCAGATCGGAAGAGCACACGTC226150.16999191345122747No Hit
ACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTC220770.165947887387254No Hit
CATTGCGATCCGTTGAAACTCATCTCAGCGAGATCGGAAGAGCACACGTC218370.16414386089031413No Hit
AAACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAAC218180.16400104212597305Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGTGACATCCGGCGCGCTTGTGGCGGCAGATCGGAAGAGCACACGTCTG218050.1639033240240555Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTGCGATCCGTTGAAACTCATCTCAGCAGATCGGAAGAGCACACGTCTG217160.16323433086477362Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTC213850.16074627765441077No Hit
TCCGTGTTAGTATAGTGGTGAGTATTCCCAGATCGGAAGAGCACACGTCT212710.15988936506836435Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCGAGTTTAAGTACATTTGAATGTGTCTAGATCGGAAGAGCACACGTC209960.1578222513739541No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAAGATCGGAAGAGCACACGTC208650.15683755357770776No Hit
TGACTTGGGCCAACGTCTATCTGAGGGTCAGATCGGAAGAGCACACGTCT203620.15305661471120466Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTTGGAGCAGAGCGCTATGTGGCATAGATCGGAAGAGCACACGTCTGA203130.15268829263474612Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGCTGGTAGAGATAAATCGATGCAGATCGGAAGAGCACACGTCTGAACT202820.15245527254555805Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGAGCTGGTAGAGATAAATCGATGCAGATCGGAAGAGCACACGTCTGAA201190.1512300378830531Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTTGGGCCAACGTCTATCTGAAGATCGGAAGAGCACACGTCTGAACTC191860.14421688487619944Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGCGAAAGGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGA189190.14220990539835385No Hit
TTGTGGTGAGTCTGGATAAACTAGCTGATCGCAGATCGGAAGAGCACACG185400.139361046888603No Hit
GAGTGATGGCCGCATCGGCGACTCAGGAGAGATCGGAAGAGCACACGTCT181150.13616641663360537Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCGAGTGTCGTAAACGGTAGAGCAATTAGATCGGAAGAGCACACGTCT179110.1346329941112065Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCGGGGGAGTGTTATAGTCCAGCCGAGATCGGAAGAGCACACGTCTGA171010.12854440468403452Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGACTTGGGCCAACGTCTATCTGAGAGATCGGAAGAGCACACGTCTGAAC170730.1283339349260582Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGAATCAACTGTAGACGACTTTAAGCAGATCGGAAGAGCACACGTCTGA170250.12797312962667023Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTAGCGTCATACCGCGTAGAATAAACAGATCGGAAGAGCACACGTCTG169200.12718386803425907Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGCTGGTAGAGATAAATCGATGCCAAAGAGATCGGAAGAGCACACGTCT158480.11912588301459444Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTGCGCATAACCGCCCGATGCGAGAGCAGATCGGAAGAGCACACGTCTG158200.11891541325661811Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGCTGGTAGAGATAAATCGATGCCAGATCGGAAGAGCACACGTCTGAAC157370.11829152075975974Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GACTTGGGCCAACGTCTATCTGAGGAGATCGGAAGAGCACACGTCTGAAC154850.11639729293797291Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAACT153700.1155328635748559Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGT151890.11417232692508042No Hit
GCGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTG151030.11352588409701031Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTTCGGGGGAGTGTTATAGTCCAGCCGAGATCGGAAGAGCACACGTCT150440.11308239424984595Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGCGATCCGTTGAAACTCATCTCAGATCGGAAGAGCACACGTCTGAAC148280.11145877040260009Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGAA146260.10994038143434237Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTCGGGGGAGTGTTATAGTCCAGCCAGATCGGAAGAGCACACGTCTGAA145850.10963219357444849Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTATACACTGACGAGTAGGAGGGCGTGTCGAGATCGGAAGAGCACACGT145450.10933152249162517No Hit
AGAGCGCTATGTGGCATATAGCTCGAGAGATCGGAAGAGCACACGTCTGA145370.10927138827506051Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAA143360.1077605160838734Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGCGATCCGTTGAAACTCATCTCAGCGAAGATCGGAAGAGCACACGTC142530.10713662358701505No Hit
CGCACGCGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACAC141790.10658038208379193No Hit
TGACTTGGGCCAACGTCTATCTGAAGATCGGAAGAGCACACGTCTGAACT141430.10630977810925095Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAACTCC141280.10619702645319219Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGCGATGGATCATTCAAGTGTCTGACCAGATCGGAAGAGCACACGTCTG140380.10552051651683976Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGAGTGTTATAGTCCAGCCGAGATCGGAAGAGCACACGTCTGAACTC138990.10447568450402878Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTAGAACATCGAAACTCTGAACGAGATCGGAAGAGCACACGTCTGAACTC137200.10313018140839447Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCCTTCGGGGGAGTGTTATAGTCCAGCCAGATCGGAAGAGCACACGTCT136920.10291971165041815Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTAAGCTGTGTGAGAATGTGATGCTAGATCGGAAGAGCACACGTCTGAA136730.10277689288607707Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGTGATGGCCGCATCGGCGACTCAGGAGGAGATCGGAAGAGCACACGTC136110.10231085270770095No Hit
CAAGTGATCCCTGGAATCGGTTTGCCCAGATCGGAAGAGCACACGTCTGA135960.1021981010516422Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCAGCTAAATGCGAGAATTAGTGTGAATTGCAGGAGATCGGAAGAGCACA135920.1021680339433599No Hit
GTGTGAGTCCCATCTGACGAGCGCGGCGAGCAGATCGGAAGAGCACACGT133810.10058199398146693No Hit
TGTGGTGAGTCTGGATAAACTAGCTGATCGCATAGATCGGAAGAGCACAC133100.10004830280945556No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)