FastQCFastQC Report
Thu 28 Sept 2023
SRR3199160.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3199160.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13354354
Total Bases681 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG2917572.184733158938276TruSeq Adapter, Index 7 (100% over 49bp)
GACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGT2614051.9574514798694118No Hit
TGACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACG2222491.6642437365371625No Hit
TTGTGACATCCGGCGCGCTTGTGGCGAGATCGGAAGAGCACACGTCTGAA1099090.823019967869655Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGTGACATCCGGCGCGCTTGTGGCAGATCGGAAGAGCACACGTCTGAAC746060.5586642378957455Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGATGGCCGCATCGGCGACTCAGGAGATCGGAAGAGCACACGTCTGAA587440.4398864969432441Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGCGATCCGTTGAAACTCATCTCAGCGAGATCGGAAGAGCACACGTCT529610.39658226822502984Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGAAC474690.3554571041025272Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTG446310.3342056081484735Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGACCTCAGATTAGTCGAGAGCACTCGCTGAGATCGGAAGAGCACACGTC438660.3284771393659326No Hit
GAGTGATGGCCGCATCGGCGACTCAGGAAGATCGGAAGAGCACACGTCTG434280.32519731018063475Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGATCGGAAGAGCACACGTCT426430.3193190775083542Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACGCGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACG425590.31869006917144777No Hit
TTGTGGTGAGTCTGGATAAACTAGCTGATCAGATCGGAAGAGCACACGTC413370.3095394955083563No Hit
AGCTGGTAGAGATAAATCGATGCAGATCGGAAGAGCACACGTCTGAACTC387700.2903173002602747Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGTGACCGGTTGGGAATACCTCGTGCGCTAAGCAAGATCGGAAGAGCACA376370.28183317590652457No Hit
GCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTGAA370010.2770706842128043Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGTGGTGAGTCTGGATAAACTAGCTGATCGCATAGATCGGAAGAGCACA365570.2737459258605845No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAGTAGATCGGAAGAGCACACG362560.2714919793200031No Hit
CGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTGA352130.2636817924700813Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGGTAGAGATAAATCGATGCCAAAGCAGATCGGAAGAGCACACGTCT347410.260147364672226Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTTGGGCCAACGTCTATCTGAGGGTAGATCGGAAGAGCACACGTCTGA330020.24712539445936507Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGA326190.24425741597085115Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGAGATCGGAAGAGCACACGT306110.2292211214409922No Hit
TCCTTCGGGGGAGTGTTATAGTCCAGCCAGATCGGAAGAGCACACGTCTG305390.2286819714379295Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGCAGAGCGCTATGTGGCATATAGCTCGAGAGATCGGAAGAGCACACGT304290.22785827004436157No Hit
GCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAACT285260.2136082359356357Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGTGATGGCCGCATCGGCGACTCAGGAGATCGGAAGAGCACACGTCTGA283050.21195334495401277Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTTGGGCCAACGTCTATCTGAGAGATCGGAAGAGCACACGTCTGAACT283020.21193088037055183Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTGACATCCGGCGCGCTTGTGGCGAGATCGGAAGAGCACACGTCTGAAC282440.21149656509030687Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGATAGGGATAGCGATCACGTAAAGTGTCACAAGATCGGAAGAGCACAC274530.20557340325110446No Hit
ATTGCGATCCGTTGAAACTCATCTCAGCGATAGATCGGAAGAGCACACGT270340.20243584976105922No Hit
ATCCGAGTGTCGTAAACGGTAGAGCAATTTAGATCGGAAGAGCACACGTC269510.20181432961863974No Hit
TGACTTGGGCCAACGTCTATCTGAGGGTAGATCGGAAGAGCACACGTCTG264770.19826492543181048Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTGGTAGAGATAAATCGATGCCAGATCGGAAGAGCACACGTCTGAACT263910.1976209407059301Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTTTGGAGCAGAGCGCTATGTGGCATATAGCTCGAGAGATCGGAAGAGC260790.1952846240259918No Hit
TGGAGCTGGTAGAGATAAATCGATGCCAGATCGGAAGAGCACACGTCTGA259880.1946031983276765Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCT251060.18799861079015878Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATAGAAGACGATCAAACTCAGTCAGATCGGAAGAGCACACGTCTGAACT241450.1808024558881695Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGTGGTGAGTCTGGATAAACTAGCTGATCGCAAGATCGGAAGAGCACAC240800.1803157232465157No Hit
GACTTGGGCCAACGTCTATCTGAGGGTCAGATCGGAAGAGCACACGTCTG240560.18013600657882814Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCCGAGTGTCGTAAACGGTAGAGCAGATCGGAAGAGCACACGTCTGAAC239090.17903524198924187Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTGACATCCGGCGCGCTTGTGGCAGATCGGAAGAGCACACGTCTGAACT238720.17875817879322353Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGCTGGTAGAGATAAATCGATGCCAAAGCAGATCGGAAGAGCACACGTC236580.1771557051730095No Hit
TGAGTCTGAATATTCGTGCATGGAATAATGAAAAGATCGGAAGAGCACAC234300.1754483968299777No Hit
CAAGTGATCCCTGGAATCGGTTTGCCCGAGATCGGAAGAGCACACGTCTG230320.17246809542415903Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTC221360.1657586731638236No Hit
TTGTGACATCCGGCGCGCTTGTGGCGGCAGATCGGAAGAGCACACGTCTG220740.1652944051056307Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAAC220470.16509222385448222Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTGCGATCCGTTGAAACTCATCTCAGCAGATCGGAAGAGCACACGTCTG219670.16449316829552368Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGCGAGTTTAAGTACATTTGAATGTGTCTAGATCGGAAGAGCACACGTC219290.1642086169050184No Hit
ACGCGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTC219190.16413373496014858No Hit
CATTGCGATCCGTTGAAACTCATCTCAGCGAGATCGGAAGAGCACACGTC217470.16284576550838775No Hit
TCCGTGTTAGTATAGTGGTGAGTATTCCCAGATCGGAAGAGCACACGTCT216060.16178993008572334Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAAGATCGGAAGAGCACACGTC215190.16113845716535596No Hit
GAGCTGGTAGAGATAAATCGATGCAGATCGGAAGAGCACACGTCTGAACT210860.15789606895249295Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTTTGGAGCAGAGCGCTATGTGGCATAGATCGGAAGAGCACACGTCTGA207920.15569453977332037Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGAGCTGGTAGAGATAAATCGATGCAGATCGGAAGAGCACACGTCTGAA203550.15242219878250943Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGACTTGGGCCAACGTCTATCTGAGGGTCAGATCGGAAGAGCACACGTCT199840.14964407862783927Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCGAAAGGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGA198120.14835610917607844No Hit
GACTTGGGCCAACGTCTATCTGAAGATCGGAAGAGCACACGTCTGAACTC195500.1463942022204893Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGTGGTGAGTCTGGATAAACTAGCTGATCGCAGATCGGAAGAGCACACG189960.1422457424747015No Hit
GAGTGATGGCCGCATCGGCGACTCAGGAGAGATCGGAAGAGCACACGTCT188460.1411225133016543Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCGAGTGTCGTAAACGGTAGAGCAATTAGATCGGAAGAGCACACGTCT179070.1340910986783786Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTAGCGTCATACCGCGTAGAATAAACAGATCGGAAGAGCACACGTCTG177280.13275071186520893Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGACTTGGGCCAACGTCTATCTGAGAGATCGGAAGAGCACACGTCTGAAC175750.1316050181087007Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGAATCAACTGTAGACGACTTTAAGCAGATCGGAAGAGCACACGTCTGA174900.1309685215773073Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTCGGGGGAGTGTTATAGTCCAGCCGAGATCGGAAGAGCACACGTCTGA170730.127845944476236Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGCTGGTAGAGATAAATCGATGCCAGATCGGAAGAGCACACGTCTGAAC167330.12529995835066227Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTGCGCATAACCGCCCGATGCGAGAGCAGATCGGAAGAGCACACGTCTG165230.12372743750839613Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGCTGGTAGAGATAAATCGATGCCAAAGAGATCGGAAGAGCACACGTCT164750.12336800417302102Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTG159690.11957897776260835Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTTGGGCCAACGTCTATCTGAGGAGATCGGAAGAGCACACGTCTGAAC157750.11812626803213394Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAACT156010.11682332219139915Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGAA153460.11491383259721885Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAA150930.11301931939201253Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTTCGGGGGAGTGTTATAGTCCAGCCGAGATCGGAAGAGCACACGTCT150880.11298187841957762Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTATACACTGACGAGTAGGAGGGCGTGTCGAGATCGGAAGAGCACACGT148740.11137940479936358No Hit
CTTCGGGGGAGTGTTATAGTCCAGCCAGATCGGAAGAGCACACGTCTGAA148710.11135694021590262Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGCGATCCGTTGAAACTCATCTCAGATCGGAAGAGCACACGTCTGAAC148540.11122964090962394Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTGCGATCCGTTGAAACTCATCTCAGCGAAGATCGGAAGAGCACACGTC147020.11009143534760274No Hit
AGAGCGCTATGTGGCATATAGCTCGAGAGATCGGAAGAGCACACGTCTGA144660.1083242214486751Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGACTTGGGCCAACGTCTATCTGAAGATCGGAAGAGCACACGTCTGAACT143760.10765028394484676Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAACTCC142680.10684155894025275Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCACGCGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACAC142530.10672923602294802No Hit
GAGTGATGGCCGCATCGGCGACTCAGGAGGAGATCGGAAGAGCACACGTC142300.10655700754974744No Hit
GGGGGAGTGTTATAGTCCAGCCGAGATCGGAAGAGCACACGTCTGAACTC142010.10633984990962499Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCAGCTAAATGCGAGAATTAGTGTGAATTGCAGGAGATCGGAAGAGCACA141920.10627245615924216No Hit
CGGCGATGGATCATTCAAGTGTCTGACCAGATCGGAAGAGCACACGTCTG140440.10516420337516887Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGTGAGTCCCATCTGACGAGCGCGGCGAGCAGATCGGAAGAGCACACGT138300.10356172975495484No Hit
CGTAAGCTGTGTGAGAATGTGATGCTAGATCGGAAGAGCACACGTCTGAA136660.10233366585908985Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGAAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGA136530.10223631933075909Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAAGTGATCCCTGGAATCGGTTTGCCCAGATCGGAAGAGCACACGTCTGA136470.1021913901638372Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTAGAACATCGAAACTCTGAACGAGATCGGAAGAGCACACGTCTGAACTC136310.1020715790520455Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCCTTCGGGGGAGTGTTATAGTCCAGCCAGATCGGAAGAGCACACGTCT134760.10091090890656336Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGGTGAGTCTGGATAAACTAGCTGATCGCATAGATCGGAAGAGCACAC134640.10082105057271959No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)