FastQCFastQC Report
Thu 28 Sept 2023
SRR3199161.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3199161.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17055621
Total Bases869.8 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGT2210541.2960771114695853No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG1531200.8977685421128905TruSeq Adapter, Index 8 (100% over 49bp)
TGACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACG1386010.8126411814615252No Hit
TTGTGACATCCGGCGCGCTTGTGGCGAGATCGGAAGAGCACACGTCTGAA1019760.5979025917613906Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGTGACATCCGGCGCGCTTGTGGCAGATCGGAAGAGCACACGTCTGAAC982660.5761502322313564Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGACCTCAGATTAGTCGAGAGCACTCGCTGAGATCGGAAGAGCACACGTC935010.5482122286840215No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAGATCGGAAGAGCACACGTCT812420.4763356315199546Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGTAGATCGGAAGAGCACACG802060.4702613877266621No Hit
ATTGCGATCCGTTGAAACTCATCTCAGCGAGATCGGAAGAGCACACGTCT666520.3907919858209795Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGAAC652310.3824604216990985Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGTGACCGGTTGGGAATACCTCGTGCGCTAAGCAAGATCGGAAGAGCACA588520.345059262280746No Hit
CGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTGA572810.3358482227061682Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGAGATCGGAAGAGCACACGT559080.3277980907291502No Hit
GAGCAGAGCGCTATGTGGCATATAGCTCGAGAGATCGGAAGAGCACACGT558630.3275342480933412No Hit
AGTCTGACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCA555490.3256932128123626No Hit
GACTTGGGCCAACGTCTATCTGAGGGTAGATCGGAAGAGCACACGTCTGA520610.3052424769523197Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTTGGGCCAACGTCTATCTGAGAGATCGGAAGAGCACACGTCTGAACT477790.28013638436266847Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGTTCTAGATCGGAAGAGCAC474410.27815463300925836No Hit
AGCTGGTAGAGATAAATCGATGCAGATCGGAAGAGCACACGTCTGAACTC471570.27648949281881907Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CATTGCGATCCGTTGAAACTCATCTCAGCGAGATCGGAAGAGCACACGTC446230.26163222083792786No Hit
AAACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAAC411570.24131047471094721Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CATAGAAGACGATCAAACTCAGTCAGATCGGAAGAGCACACGTCTGAACT407830.23911764924888987Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAACTCC403840.23677824454471635Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCCGAGTGTCGTAAACGGTAGAGCAATTTAGATCGGAAGAGCACACGTC389960.22864016502242868No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAAGATCGGAAGAGCACACGTC378660.2220147832787795No Hit
GAGCTGGTAGAGATAAATCGATGCAGATCGGAAGAGCACACGTCTGAACT377670.22143432947999958Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAACT371370.21774053257867304Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAACT369400.2165854881507979Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGTGTTAGTATAGTGGTGAGTATTCCCAGATCGGAAGAGCACACGTCT363770.2132845236183426Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTTGGGCCAACGTCTATCTGAGGGTCAGATCGGAAGAGCACACGTCTG356660.20911580997255977Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGTGAGTCCCATCTGACGAGCGCGGCGAGCAGATCGGAAGAGCACACGT352450.20664741553532412No Hit
TTGTGGTGAGTCTGGATAAACTAGCTGATCAGATCGGAAGAGCACACGTC348510.20433732667957386No Hit
GCTTAGCGTCATACCGCGTAGAATAAACAGATCGGAAGAGCACACGTCTG347310.2036337463174164Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCCGAGTGTCGTAAACGGTAGAGCAGATCGGAAGAGCACACGTCTGAAC341180.2000396233007288Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGACTTGGGCCAACGTCTATCTGAGGGTAGATCGGAAGAGCACACGTCTG337950.1981458194925884Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTG337440.19784679783867148Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGCGATGGATCATTCAAGTGTCTGACCAGATCGGAAGAGCACACGTCTG329070.19293932481262335Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTTCGGGGGAGTGTTATAGTCCAGCCAGATCGGAAGAGCACACGTCTG315180.18479538212065102Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACGCGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACG312990.1835113479597137No Hit
GCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTGAA304890.17876218051515097Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGCGATCCGTTGAAACTCATCTCAGCAGATCGGAAGAGCACACGTCTG301530.17679215550111016Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGA297660.17452310883315242Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAAGTGATCCCTGGAATCGGTTTGCCCGAGATCGGAAGAGCACACGTCTG297080.174183044991443Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTGGTAGAGATAAATCGATGCCAGATCGGAAGAGCACACGTCTGAACT294890.17289901083050566Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGCGAAAGGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGA291530.17092898581646485No Hit
GGAGCAGAGCGCTATGTGGCATATAGCTCGAGAGATCGGAAGAGCACACG281350.1649602790774959No Hit
GTGCGAGTTTAAGTACATTTGAATGTGTCTAGATCGGAAGAGCACACGTC274880.1611668082915304No Hit
GAGATAGGGATAGCGATCACGTAAAGTGTCACAAGATCGGAAGAGCACAC273620.16042804891126508No Hit
GAAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGAA270480.15858701363028646Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGA265800.15584305021787245Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGCGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTC262080.15366195109518438No Hit
ATTGCGATCCGTTGAAACTCATCTCAGCGATAGATCGGAAGAGCACACGT253250.1484847722636426No Hit
TGACTTGGGCCAACGTCTATCTGAGAGATCGGAAGAGCACACGTCTGAAC251170.14726523296923635Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTG250200.14669650550982574Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGGTGGGAGCTCCGGCGACAATGAAATACCACTACTAGATCGGAAGAGC248450.14567045081501284No Hit
GGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAA247450.1450841338465483Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGTGGTGAGTCTGGATAAACTAGCTGATCGCATAGATCGGAAGAGCACA243600.14282681351795987No Hit
CTTCGGGGGAGTGTTATAGTCCAGCCAGATCGGAAGAGCACACGTCTGAA241000.14130238939995207Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCT240340.1409154202007655Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGCTGGTAGAGATAAATCGATGCCAGATCGGAAGAGCACACGTCTGAAC238030.13956102800361242Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGACTTGGGCCAACGTCTATCTGAGGGTCAGATCGGAAGAGCACACGTCT236760.13881640545366247Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTTGGGCCAACGTCTATCTGAGGAGATCGGAAGAGCACACGTCTGAAC235360.1379955616978121Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGCGAGTTTAAGTACATTTGAATGTGTCTCTACCAGATCGGAAGAGCAC225280.13208548665568964No Hit
GACTTGGGCCAACGTCTATCTGAAGATCGGAAGAGCACACGTCTGAACTC221070.12961709221845397Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCT219140.12848550046931742Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGTGTTAGTATAGTGGTGAGTATTCCCGAGATCGGAAGAGCACACGTC218070.12785814131306036No Hit
TGTGACATCCGGCGCGCTTGTGGCAGATCGGAAGAGCACACGTCTGAACT216630.12701384487847142Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGTGTTAGTATAGTGGTGAGTATTCCAGATCGGAAGAGCACACGTCTG211330.12390636494560943Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTGGTAGAGATAAATCGATGCCAAAGCAGATCGGAAGAGCACACGTCT206970.12135002296310407Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCTTCGGGGGAGTGTTATAGTCCAGCCAGATCGGAAGAGCACACGTCT206490.1210685908182411Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGACCTCAGATTAGTCGAGAGCACTCGCTGAGATCGGAAGAGCACACGT205140.12027706291081398No Hit
CGGCGATGGATCATTCAAGTGTCTGACCTATCAGATCGGAAGAGCACACG195730.11475982023756275No Hit
TGACTTGGGCCAACGTCTATCTGAAGATCGGAAGAGCACACGTCTGAACT194680.11414418742067498Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGTGACATCCGGCGCGCTTGTGGCGGCAGATCGGAAGAGCACACGTCTG193470.11343474388883289Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGAATCAACTGTAGACGACTTTAAGCAGATCGGAAGAGCACACGTCTGA192060.1126080369632979Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCCGAGTGTCGTAAACGGTAGAGCAATTAGATCGGAAGAGCACACGTCT191790.11244973138181248Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTCTGACTTGGGCCAACGTCTATCTGAGGGTCAGATCGGAAGAGCACAC190470.1116757929834393No Hit
GAGCTGGTAGAGATAAATCGATGCCAAAGCAGATCGGAAGAGCACACGTC189530.11112465503308265No Hit
TGTGAGTCCCATCTGACGAGCGCGGCGAGCAGATCGGAAGAGCACACGTC188410.11046798002840236No Hit
CTTCGGGGGAGTGTTATAGTCCAGCCGAGATCGGAAGAGCACACGTCTGA186730.10948296752138197Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTGGGTGAGTCGTGCGAGTTTAAGTACAGATCGGAAGAGCACACGTCT185820.10894941908007924Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGTTCAGATCGGAAGAGCACA184720.10830447041476825No Hit
GGTGCGAAAGGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAG181820.1066041512062211No Hit
ACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTC181020.10613509763144947No Hit
ATTGCGATCCGTTGAAACTCATCTCAGATCGGAAGAGCACACGTCTGAAC180560.10586539182595579Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTTGGAGCAGAGCGCTATGTGGCATATAGCTCGAGAGATCGGAAGAGC178010.10437028355637125No Hit
GGGCGACCGGGGTTCGATTCCCCGACACGGAGATCGGAAGAGCACACGTC176700.1036022083276827No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)