FastQCFastQC Report
Thu 28 Sept 2023
SRR3199162.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3199162.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17077465
Total Bases870.9 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGT2216841.2981083550749482No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG1532640.8974634115777723TruSeq Adapter, Index 8 (100% over 49bp)
TGACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACG1383180.8099445672996548No Hit
TTGTGACATCCGGCGCGCTTGTGGCGAGATCGGAAGAGCACACGTCTGAA1017620.5958846936591584Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGTGACATCCGGCGCGCTTGTGGCAGATCGGAAGAGCACACGTCTGAAC986390.5775974361534337Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGACCTCAGATTAGTCGAGAGCACTCGCTGAGATCGGAAGAGCACACGTC934830.5474056014753946No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAGATCGGAAGAGCACACGTCT823510.4822202826941821Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGTAGATCGGAAGAGCACACG810960.4748714168057144No Hit
ATTGCGATCCGTTGAAACTCATCTCAGCGAGATCGGAAGAGCACACGTCT672970.39406902605275435Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGAAC654730.383388283916846Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGTGACCGGTTGGGAATACCTCGTGCGCTAAGCAAGATCGGAAGAGCACA595100.34847092352407105No Hit
CGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTGA575280.3368649855233198Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGCAGAGCGCTATGTGGCATATAGCTCGAGAGATCGGAAGAGCACACGT562940.32963908870549585No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAGAGATCGGAAGAGCACACGT557150.3262486557577486No Hit
AGTCTGACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCA551400.322881645490124No Hit
GACTTGGGCCAACGTCTATCTGAGGGTAGATCGGAAGAGCACACGTCTGA527430.3088456044266523Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTTGGGCCAACGTCTATCTGAGAGATCGGAAGAGCACACGTCTGAACT482760.28268832639973207Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGTTCTAGATCGGAAGAGCAC477520.2796199553036706No Hit
AGCTGGTAGAGATAAATCGATGCAGATCGGAAGAGCACACGTCTGAACTC472110.2764520378170882Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CATTGCGATCCGTTGAAACTCATCTCAGCGAGATCGGAAGAGCACACGTC446120.2612331514074249No Hit
AAACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAAC412530.24156395577446654Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CATAGAAGACGATCAAACTCAGTCAGATCGGAAGAGCACACGTCTGAACT408950.2394676259034933Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAACTCC408620.2392743887924818Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCCGAGTGTCGTAAACGGTAGAGCAATTTAGATCGGAAGAGCACACGTC391590.22930218273028227No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAAGATCGGAAGAGCACACGTC383540.22458836835560783No Hit
GAGCTGGTAGAGATAAATCGATGCAGATCGGAAGAGCACACGTCTGAACT378190.22145558488920924Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAACT371650.21762597668916317Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAACT367630.21527199733684127Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGTGTTAGTATAGTGGTGAGTATTCCCAGATCGGAAGAGCACACGTCT365530.21404230663040447Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTTGGGCCAACGTCTATCTGAGGGTCAGATCGGAAGAGCACACGTCTG358500.20992577059885642Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGTGAGTCCCATCTGACGAGCGCGGCGAGCAGATCGGAAGAGCACACGT354900.20781772938782192No Hit
GCTTAGCGTCATACCGCGTAGAATAAACAGATCGGAAGAGCACACGTCTG347840.20368362634618195Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGTGGTGAGTCTGGATAAACTAGCTGATCAGATCGGAAGAGCACACGTC345550.20234267790916277No Hit
TGACTTGGGCCAACGTCTATCTGAGGGTAGATCGGAAGAGCACACGTCTG338000.19792164703602086Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCCGAGTGTCGTAAACGGTAGAGCAGATCGGAAGAGCACACGTCTGAAC336880.19726581199258791Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTG334060.19561451304394417Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGCGATGGATCATTCAAGTGTCTGACCAGATCGGAAGAGCACACGTCTG327280.19164436876316246Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACGCGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACG318800.18667876057716998No Hit
TCCTTCGGGGGAGTGTTATAGTCCAGCCAGATCGGAAGAGCACACGTCTG312680.18309509051841125Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTGAA310780.18198251321258746Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGCGATCCGTTGAAACTCATCTCAGCAGATCGGAAGAGCACACGTCTG303100.1774853586290471Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTGGTAGAGATAAATCGATGCCAGATCGGAAGAGCACACGTCTGAACT296290.17349764733817344Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGAAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGA294060.17219183292133813Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGCGAAAGGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGA292440.1712432143763726No Hit
CAAGTGATCCCTGGAATCGGTTTGCCCGAGATCGGAAGAGCACACGTCTG291880.17091529685465612Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAGCAGAGCGCTATGTGGCATATAGCTCGAGAGATCGGAAGAGCACACG284030.1663185958805947No Hit
GAGATAGGGATAGCGATCACGTAAAGTGTCACAAGATCGGAAGAGCACAC275490.1613178536744183No Hit
GTGCGAGTTTAAGTACATTTGAATGTGTCTAGATCGGAAGAGCACACGTC271820.15916882277316918No Hit
GAAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGAA269710.15793327639670174Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGA264490.15487661664070165Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGCGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTC258520.1513807816324027No Hit
GCGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTG254120.14880428681891603Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTGCGATCCGTTGAAACTCATCTCAGCGATAGATCGGAAGAGCACACGT251390.1472056889005482No Hit
TAGGTGGGAGCTCCGGCGACAATGAAATACCACTACTAGATCGGAAGAGC250470.14666696725772824No Hit
TGACTTGGGCCAACGTCTATCTGAGAGATCGGAAGAGCACACGTCTGAAC248040.14524403944027992Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAA245500.14375669925249443Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTCGGGGGAGTGTTATAGTCCAGCCAGATCGGAAGAGCACACGTCTGAA242410.14194729721302313Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGTGGTGAGTCTGGATAAACTAGCTGATCGCATAGATCGGAAGAGCACA241030.14113921474879323No Hit
CGCGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCT240870.1410455240283028Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGCTGGTAGAGATAAATCGATGCCAGATCGGAAGAGCACACGTCTGAAC239210.14007348280321463Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GACTTGGGCCAACGTCTATCTGAGGAGATCGGAAGAGCACACGTCTGAAC235320.1377956271612912Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGACTTGGGCCAACGTCTATCTGAGGGTCAGATCGGAAGAGCACACGTCT234030.13704024572733717Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCGAGTTTAAGTACATTTGAATGTGTCTCTACCAGATCGGAAGAGCAC225000.13175257568965884No Hit
TCCGTGTTAGTATAGTGGTGAGTATTCCCGAGATCGGAAGAGCACACGTC220150.12891257572479287No Hit
GACTTGGGCCAACGTCTATCTGAAGATCGGAAGAGCACACGTCTGAACTC220070.12886573036454768Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCT218940.12820403965108404Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGACATCCGGCGCGCTTGTGGCAGATCGGAAGAGCACACGTCTGAACT218540.127969812849858Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGTGTTAGTATAGTGGTGAGTATTCCAGATCGGAAGAGCACACGTCTG215570.12623067885075448Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTGGTAGAGATAAATCGATGCCAAAGCAGATCGGAAGAGCACACGTCT208300.12197360673847084Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCTTCGGGGGAGTGTTATAGTCCAGCCAGATCGGAAGAGCACACGTCT203750.1193092768745244Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGACCTCAGATTAGTCGAGAGCACTCGCTGAGATCGGAAGAGCACACGT203310.11905162739317575No Hit
TGACTTGGGCCAACGTCTATCTGAAGATCGGAAGAGCACACGTCTGAACT195050.11421484394785761Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGTGACATCCGGCGCGCTTGTGGCGGCAGATCGGAAGAGCACACGTCTG193780.11347117385396485Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTCTGACTTGGGCCAACGTCTATCTGAGGGTCAGATCGGAAGAGCACAC193250.11316082334234033No Hit
TTGAATCAACTGTAGACGACTTTAAGCAGATCGGAAGAGCACACGTCTGA192320.11261624602948975Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCCGAGTGTCGTAAACGGTAGAGCAATTAGATCGGAAGAGCACACGTCT191990.11242300891847823Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGCGATGGATCATTCAAGTGTCTGACCTATCAGATCGGAAGAGCACACG191860.11234688520807977No Hit
CTTCGGGGGAGTGTTATAGTCCAGCCGAGATCGGAAGAGCACACGTCTGA190470.11153294707381921Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGAGTCCCATCTGACGAGCGCGGCGAGCAGATCGGAAGAGCACACGTC190340.11145682336342075No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAGTTCAGATCGGAAGAGCACA187440.10975867905453181No Hit
GAGCTGGTAGAGATAAATCGATGCCAAAGCAGATCGGAAGAGCACACGTC187190.10961228730376552No Hit
CAGTGGGTGAGTCGTGCGAGTTTAAGTACAGATCGGAAGAGCACACGTCT183290.10732857599181143Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTC181070.1060286172450068No Hit
ATTGCGATCCGTTGAAACTCATCTCAGATCGGAAGAGCACACGTCTGAAC180010.10540791622175774Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGTGCGAAAGGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAG176660.10344626676148949No Hit
TTTTTGGAGCAGAGCGCTATGTGGCATATAGCTCGAGAGATCGGAAGAGC176350.10326474099053928No Hit
GGGCGACCGGGGTTCGATTCCCCGACACGGAGATCGGAAGAGCACACGTC176300.10323546264038602No Hit
CATTGCGATCCGTTGAAACTCATCTCAGCAGATCGGAAGAGCACACGTCT173870.10181253482293771Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)