FastQCFastQC Report
Thu 28 Sept 2023
SRR3199163.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3199163.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13109192
Total Bases668.5 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGT1566731.1951384951871937No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG1142830.8717776045998868TruSeq Adapter, Index 8 (100% over 49bp)
TGACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACG1012850.7726258033294501No Hit
TTGTGACATCCGGCGCGCTTGTGGCGAGATCGGAAGAGCACACGTCTGAA783590.5977408828858407Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGTGACATCCGGCGCGCTTGTGGCAGATCGGAAGAGCACACGTCTGAAC718970.5484472269534233Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGACCTCAGATTAGTCGAGAGCACTCGCTGAGATCGGAAGAGCACACGTC715680.5459375375690584No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAGATCGGAAGAGCACACGTCT590400.45037100684771414Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGTAGATCGGAAGAGCACACG557760.4254724471195479No Hit
AAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGAAC518670.3956536756803928Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTGCGATCCGTTGAAACTCATCTCAGCGAGATCGGAAGAGCACACGTCT513600.39178616042849934Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTGA456490.3482213091394191Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGCAGAGCGCTATGTGGCATATAGCTCGAGAGATCGGAAGAGCACACGT447790.3415847445059924No Hit
GGTGACCGGTTGGGAATACCTCGTGCGCTAAGCAAGATCGGAAGAGCACA407160.31059122484436874No Hit
AGTCTGACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCA396320.30232221787582336No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAGAGATCGGAAGAGCACACGT395450.30165856141248065No Hit
AGCTGGTAGAGATAAATCGATGCAGATCGGAAGAGCACACGTCTGAACTC367400.2802613616460877Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GACTTGGGCCAACGTCTATCTGAGGGTAGATCGGAAGAGCACACGTCTGA361700.2759132675759116Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CATTGCGATCCGTTGAAACTCATCTCAGCGAGATCGGAAGAGCACACGTC357590.2727780629042583No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAGTTCTAGATCGGAAGAGCAC347310.2649362371075197No Hit
GACTTGGGCCAACGTCTATCTGAGAGATCGGAAGAGCACACGTCTGAACT345480.2635402700639368Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAACTCC311120.23732965387950686Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CATAGAAGACGATCAAACTCAGTCAGATCGGAAGAGCACACGTCTGAACT304460.23224924922909054Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAAC301590.23005994572358082Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAACT276710.21108089651902268Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCCGAGTGTCGTAAACGGTAGAGCAATTTAGATCGGAAGAGCACACGTC275360.21005108476555992No Hit
GAGCTGGTAGAGATAAATCGATGCAGATCGGAAGAGCACACGTCTGAACT270550.20638190362914816Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAAGATCGGAAGAGCACACGTC268920.205138501289782No Hit
GCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAACT268330.20468843541234272Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGTGAGTCCCATCTGACGAGCGCGGCGAGCAGATCGGAAGAGCACACGT260310.19857059077325284No Hit
TCCGTGTTAGTATAGTGGTGAGTATTCCCAGATCGGAAGAGCACACGTCT258810.19742635549162757Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGTGGTGAGTCTGGATAAACTAGCTGATCAGATCGGAAGAGCACACGTC254280.19397076494111917No Hit
GCACGCGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACG253150.1931087743622948No Hit
GACTTGGGCCAACGTCTATCTGAGGGTCAGATCGGAAGAGCACACGTCTG252430.19255954142711465Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTAGCGTCATACCGCGTAGAATAAACAGATCGGAAGAGCACACGTCTG251240.19165178143702527Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTG243830.18599925914579632Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGACTTGGGCCAACGTCTATCTGAGGGTAGATCGGAAGAGCACACGTCTG240170.1832073250586306Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCCGAGTGTCGTAAACGGTAGAGCAGATCGGAAGAGCACACGTCTGAAC236410.18033910861935656Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTGAA232360.17724967335896827Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGCGATCCGTTGAAACTCATCTCAGCAGATCGGAAGAGCACACGTCTG230760.17602915572523462Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTGGTAGAGATAAATCGATGCCAGATCGGAAGAGCACACGTCTGAACT228640.17441196986053756Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGCGATGGATCATTCAAGTGTCTGACCAGATCGGAAGAGCACACGTCTG227770.1737483133971949Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGA219570.16749316052430996Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTTCGGGGGAGTGTTATAGTCCAGCCAGATCGGAAGAGCACACGTCTG218550.16671508053280476Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGCGAAAGGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGA216360.16504449702163185No Hit
GGAGCAGAGCGCTATGTGGCATATAGCTCGAGAGATCGGAAGAGCACACG212770.162305960580942No Hit
GAAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGAA208050.15870543356142774Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGCGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTC205360.15665343828971307No Hit
CAAGTGATCCCTGGAATCGGTTTGCCCGAGATCGGAAGAGCACACGTCTG204140.15572279359399116Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTGCGATCCGTTGAAACTCATCTCAGCGATAGATCGGAAGAGCACACGT201900.15401406890676406No Hit
GTGCGAGTTTAAGTACATTTGAATGTGTCTAGATCGGAAGAGCACACGTC198100.15111533952664663No Hit
CGCGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCT195500.14913199837182947Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGATAGGGATAGCGATCACGTAAAGTGTCACAAGATCGGAAGAGCACAC194020.14800301956062586No Hit
TGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGA191100.14577557487906195Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTG189050.14421178666084072Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGACTTGGGCCAACGTCTATCTGAGGGTCAGATCGGAAGAGCACACGTCT184150.14047395140753147Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGACTTGGGCCAACGTCTATCTGAGAGATCGGAAGAGCACACGTCTGAAC183360.13987132082587547Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGGTGGGAGCTCCGGCGACAATGAAATACCACTACTAGATCGGAAGAGC181610.1385363796639793No Hit
TTGTGGTGAGTCTGGATAAACTAGCTGATCGCATAGATCGGAAGAGCACA173140.13207526444040182No Hit
GACTTGGGCCAACGTCTATCTGAGGAGATCGGAAGAGCACACGTCTGAAC171920.13114461974467992Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGCTGGTAGAGATAAATCGATGCCAGATCGGAAGAGCACACGTCTGAAC171890.13112173503904742Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTCGGGGGAGTGTTATAGTCCAGCCAGATCGGAAGAGCACACGTCTGAA170910.13037416798838555Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAA169200.12906973976733271Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGTGTTAGTATAGTGGTGAGTATTCCCGAGATCGGAAGAGCACACGTC166200.12678126920408214No Hit
CTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCT165440.12620152332805867Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTGGTAGAGATAAATCGATGCCAAAGCAGATCGGAAGAGCACACGTCT163800.12495049275348168Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGACATCCGGCGCGCTTGTGGCAGATCGGAAGAGCACACGTCTGAACT161130.12291375395218865Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACTTGGGCCAACGTCTATCTGAAGATCGGAAGAGCACACGTCTGAACTC157480.1201294481002338Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGCGAGTTTAAGTACATTTGAATGTGTCTCTACCAGATCGGAAGAGCAC155630.11871822458622927No Hit
TTGACCTCAGATTAGTCGAGAGCACTCGCTGAGATCGGAAGAGCACACGT154500.11785623400740487No Hit
TGTGAGTCCCATCTGACGAGCGCGGCGAGCAGATCGGAAGAGCACACGTC151780.11578135403005768No Hit
TCCGTGTTAGTATAGTGGTGAGTATTCCAGATCGGAAGAGCACACGTCTG150910.11511769756671503Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCTTCGGGGGAGTGTTATAGTCCAGCCAGATCGGAAGAGCACACGTCT149070.11371410228792132Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGTGACATCCGGCGCGCTTGTGGCGGCAGATCGGAAGAGCACACGTCTG147860.11279108582741026Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGACTTGGGCCAACGTCTATCTGAAGATCGGAAGAGCACACGTCTGAACT141520.10795478470374069Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTGCGATCCGTTGAAACTCATCTCAGATCGGAAGAGCACACGTCTGAAC141020.10757337294319894Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGAATCAACTGTAGACGACTTTAAGCAGATCGGAAGAGCACACGTCTGA139470.10639099648551947Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCTGACTTGGGCCAACGTCTATCTGAGGGTCAGATCGGAAGAGCACAC139200.10618503413482691No Hit
TTTTTGGAGCAGAGCGCTATGTGGCATATAGCTCGAGAGATCGGAAGAGC138800.10587990472639353No Hit
CGGCGATGGATCATTCAAGTGTCTGACCTATCAGATCGGAAGAGCACACG136270.1039499612180522No Hit
CATTGCGATCCGTTGAAACTCATCTCAGCAGATCGGAAGAGCACACGTCT135260.10317950946175782Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGCTGGTAGAGATAAATCGATGCCAAAGCAGATCGGAAGAGCACACGTC134780.10281335417163774No Hit
CTTCGGGGGAGTGTTATAGTCCAGCCGAGATCGGAAGAGCACACGTCTGA134070.10227174947166844Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCACGCGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACAC133790.10205815888576504No Hit
ACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTC133170.10158520830269326No Hit
ATCCGAGTGTCGTAAACGGTAGAGCAATTAGATCGGAAGAGCACACGTCT132430.10102071889709144Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)