FastQCFastQC Report
Thu 28 Sept 2023
SRR3199164.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3199164.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13154542
Total Bases670.8 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGT1623521.2341896814043394No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG1145100.8704978098059211TruSeq Adapter, Index 8 (100% over 49bp)
TGACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACG1027310.780954593478055No Hit
TTGTGACATCCGGCGCGCTTGTGGCGAGATCGGAAGAGCACACGTCTGAA800710.6086947002791887Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGACCTCAGATTAGTCGAGAGCACTCGCTGAGATCGGAAGAGCACACGTC730480.5553062964867952No Hit
TTGTGACATCCGGCGCGCTTGTGGCAGATCGGAAGAGCACACGTCTGAAC721470.5484569512188261Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGATCGGAAGAGCACACGTCT613950.46672092422526Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGTAGATCGGAAGAGCACACG580050.4409503576787394No Hit
AAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGAAC524900.39902567493417856Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTGCGATCCGTTGAAACTCATCTCAGCGAGATCGGAAGAGCACACGTCT516620.3927312710697187Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGCAGAGCGCTATGTGGCATATAGCTCGAGAGATCGGAAGAGCACACGT465670.3539994018795941No Hit
CGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTGA458800.3487768711369807Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTGACCGGTTGGGAATACCTCGTGCGCTAAGCAAGATCGGAAGAGCACA419410.31883284115858995No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAGAGATCGGAAGAGCACACGT412310.3134354658641859No Hit
AGTCTGACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCA397750.3023670455421405No Hit
GACTTGGGCCAACGTCTATCTGAGGGTAGATCGGAAGAGCACACGTCTGA376540.2862433370922378Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGGTAGAGATAAATCGATGCAGATCGGAAGAGCACACGTCTGAACTC372400.2830961351600078Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGTTCTAGATCGGAAGAGCAC361310.2746655869888895No Hit
GACTTGGGCCAACGTCTATCTGAGAGATCGGAAGAGCACACGTCTGAACT361260.2746275773037176Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CATTGCGATCCGTTGAAACTCATCTCAGCGAGATCGGAAGAGCACACGTC361070.27448314050006456No Hit
CTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAACTCC314220.2388680654940324Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CATAGAAGACGATCAAACTCAGTCAGATCGGAAGAGCACACGTCTGAACT307010.23338706889225028Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAAC298690.22706225727965293Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGCTGGTAGAGATAAATCGATGCAGATCGGAAGAGCACACGTCTGAACT283790.21573537109843885Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAAGATCGGAAGAGCACACGTC282420.2146939057247299No Hit
GCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAACT280820.21347759579923042Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACTTATACTCAGCCGTCAGTGTAAGATCGGAAGAGCACACGTCTGAACT277690.211098189507472Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGTGAGTCCCATCTGACGAGCGCGGCGAGCAGATCGGAAGAGCACACGT274080.20835389023806378No Hit
ATCCGAGTGTCGTAAACGGTAGAGCAATTTAGATCGGAAGAGCACACGTC273220.2077001236531078No Hit
TCCGTGTTAGTATAGTGGTGAGTATTCCCAGATCGGAAGAGCACACGTCT265560.20187703988477895Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTAGCGTCATACCGCGTAGAATAAACAGATCGGAAGAGCACACGTCTG262960.19990053625584228Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTTGGGCCAACGTCTATCTGAGGGTCAGATCGGAAGAGCACACGTCTG262760.19974849751515483Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACGCGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACG262500.19955084715226118No Hit
TTGTGGTGAGTCTGGATAAACTAGCTGATCAGATCGGAAGAGCACACGTC259440.1972246544197434No Hit
TTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTG252950.19229099728443605Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGACTTGGGCCAACGTCTATCTGAGGGTAGATCGGAAGAGCACACGTCTG243630.18520599196840148Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTGAA238730.18148104282155927Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCCGAGTGTCGTAAACGGTAGAGCAGATCGGAAGAGCACACGTCTGAAC237410.1804775871330222Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTGCGATCCGTTGAAACTCATCTCAGCAGATCGGAAGAGCACACGTCTG233330.17737599682299846Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGA232120.17645616244183948Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGCGATGGATCATTCAAGTGTCTGACCAGATCGGAAGAGCACACGTCTG231560.17603045396791464Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTGGTAGAGATAAATCGATGCCAGATCGGAAGAGCACACGTCTGAACT228890.17400073677973737Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGCGAAAGGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGA226790.17240433000251928No Hit
TCCTTCGGGGGAGTGTTATAGTCCAGCCAGATCGGAAGAGCACACGTCTG222580.16920391451104871Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAGCAGAGCGCTATGTGGCATATAGCTCGAGAGATCGGAAGAGCACACG219480.16684731403039346No Hit
GAAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGAA214740.1632439958761012Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGCGATCCGTTGAAACTCATCTCAGCGATAGATCGGAAGAGCACACGT207160.15748172760404733No Hit
ACGCGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTC206890.15727647530411928No Hit
CAAGTGATCCCTGGAATCGGTTTGCCCGAGATCGGAAGAGCACACGTCTG204750.15564966077876372Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGATAGGGATAGCGATCACGTAAAGTGTCACAAGATCGGAAGAGCACAC203820.15494268063456712No Hit
GTGCGAGTTTAAGTACATTTGAATGTGTCTAGATCGGAAGAGCACACGTC201350.1530650021870773No Hit
GCGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTG199840.15191710969488714Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGA196100.14907398524403206Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCT190990.14518939541946804Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGACTTGGGCCAACGTCTATCTGAGGGTCAGATCGGAAGAGCACACGTCT186400.14170010632069135Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGACTTGGGCCAACGTCTATCTGAGAGATCGGAAGAGCACACGTCTGAAC185800.14124399009862904Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGGTGGGAGCTCCGGCGACAATGAAATACCACTACTAGATCGGAAGAGC185050.14067384482105116No Hit
TTGTGGTGAGTCTGGATAAACTAGCTGATCGCATAGATCGGAAGAGCACA180150.13694889567420895No Hit
GAGCTGGTAGAGATAAATCGATGCCAGATCGGAAGAGCACACGTCTGAAC179340.13633313877442482Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAA177530.13495718817120353Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTTGGGCCAACGTCTATCTGAGGAGATCGGAAGAGCACACGTCTGAAC177200.13470632424906925Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTCGGGGGAGTGTTATAGTCCAGCCAGATCGGAAGAGCACACGTCTGAA170220.1294001721990777Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGTGTTAGTATAGTGGTGAGTATTCCCGAGATCGGAAGAGCACACGTC167300.12718040658504112No Hit
AGCTGGTAGAGATAAATCGATGCCAAAGCAGATCGGAAGAGCACACGTCT166790.12679270779628818Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGACATCCGGCGCGCTTGTGGCAGATCGGAAGAGCACACGTCTGAACT166020.12620735864464153Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCT164490.12504426227838264Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCGAGTTTAAGTACATTTGAATGTGTCTCTACCAGATCGGAAGAGCAC163570.12434488407122041No Hit
TTGACCTCAGATTAGTCGAGAGCACTCGCTGAGATCGGAAGAGCACACGT160680.12214792426828697No Hit
GACTTGGGCCAACGTCTATCTGAAGATCGGAAGAGCACACGTCTGAACTC160650.12212511845718382Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCGTGTTAGTATAGTGGTGAGTATTCCAGATCGGAAGAGCACACGTCTG154950.11779201434759189Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTGAGTCCCATCTGACGAGCGCGGCGAGCAGATCGGAAGAGCACACGTC151870.11545061774100535No Hit
TTGTGACATCCGGCGCGCTTGTGGCGGCAGATCGGAAGAGCACACGTCTG151640.1152757731892148Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCTTCGGGGGAGTGTTATAGTCCAGCCAGATCGGAAGAGCACACGTCT150940.11474363759680878Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGAATCAACTGTAGACGACTTTAAGCAGATCGGAAGAGCACACGTCTGA142990.10870009765448314Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGACTTGGGCCAACGTCTATCTGAAGATCGGAAGAGCACACGTCTGAACT141900.1078714865177366Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTCTGACTTGGGCCAACGTCTATCTGAGGGTCAGATCGGAAGAGCACAC141780.10778026327332416No Hit
TTTTTGGAGCAGAGCGCTATGTGGCATATAGCTCGAGAGATCGGAAGAGC141430.10751419547712113No Hit
GAGCTGGTAGAGATAAATCGATGCCAAAGCAGATCGGAAGAGCACACGTC140890.10710369087726505No Hit
ATTGCGATCCGTTGAAACTCATCTCAGATCGGAAGAGCACACGTCTGAAC140640.10691364245140576Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGCGATGGATCATTCAAGTGTCTGACCTATCAGATCGGAAGAGCACACG139270.10587217707769682No Hit
CATTGCGATCCGTTGAAACTCATCTCAGCAGATCGGAAGAGCACACGTCT138260.10510438143722525Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCGAGTGTCGTAAACGGTAGAGCAATTAGATCGGAAGAGCACACGTCT136710.10392608119689761Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGTTCAGATCGGAAGAGCACA135490.10299864487870426No Hit
GGTGCGAAAGGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAG134760.10244370347519512No Hit
CTTCGGGGGAGTGTTATAGTCCAGCCGAGATCGGAAGAGCACACGTCTGA134410.1021776356789921Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCACGCGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACAC134250.10205600468644214No Hit
ACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTC133660.1016074904014142No Hit
CAGTGGGTGAGTCGTGCGAGTTTAAGTACAGATCGGAAGAGCACACGTCT131920.10028475335743349Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)