FastQCFastQC Report
Thu 28 Sept 2023
SRR3199173.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3199173.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18023800
Total Bases919.2 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGCGTCATACCGCGTAGAATAAACCAGATCGGAAGAGCACACGTCT1171400.6499184411722279Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGACCTCAGATTAGTCGAGAGCACTCGCTGAGATCGGAAGAGCACACGTC819910.45490407128352506No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAAGATCGGAAGAGCACACGTC764960.4244166047115481No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG677590.3759418102730834TruSeq Adapter, Index 10 (100% over 49bp)
GATAGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCT643560.3570612190548053Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGATAGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTC584710.32440994684805646No Hit
GACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGT568140.31521654701006446No Hit
AAAGGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGAGCACA556180.3085808763967643No Hit
AAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGAAC528480.29321230817030813Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTTAGCGTCATACCGCGTAGAATAAACAGATCGGAAGAGCACACGTCTG503070.27911428222683343Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGTGATGGCCGCATCGGCGACTCAGGAGATCGGAAGAGCACACGTCTGA411460.22828704268800143Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGAGATCGGAAGAGCACACGT395850.21962627192933787No Hit
GACTTGGGCCAACGTCTATCTGAGGGTAGATCGGAAGAGCACACGTCTGA381480.21165348039814025Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTGCGATCCGTTGAAACTCATCTCAGCGAGATCGGAAGAGCACACGTCT366440.20330895815532796Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTTGGGCCAACGTCTATCTGAGAGATCGGAAGAGCACACGTCTGAACT358290.19878715920061252Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCTGGTAGAGATAAATCGATGCAGATCGGAAGAGCACACGTCTGAACTC353970.19639032834363454Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCCGAGTGTCGTAAACGGTAGAGCAGATCGGAAGAGCACACGTCTGAAC328640.1823366881567705Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGTGTTAGTATAGTGGTGAGTATTCCCGAGATCGGAAGAGCACACGTC313870.17414196784251934No Hit
GGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGAGCACACGT311190.17265504499606077No Hit
ATTGCGATCCGTTGAAACTCATCTCAGCAGATCGGAAGAGCACACGTCTG310640.17234989291936217Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGTGACATCCGGCGCGCTTGTGGCGAGATCGGAAGAGCACACGTCTGAA290890.16139215925609474Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTACAATGAGTCGATCAACGTGCGAGATCGGAAGAGCACACGTCTGAAC285980.1586679834441128Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GACTTGGGCCAACGTCTATCTGAGGGTCAGATCGGAAGAGCACACGTCTG283420.15724763923257026Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTACAATGAGTCGATCAACGTGCGTATAGATCGGAAGAGCACACGTCTG283310.15718660881723054Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTGACCGGTTGGGAATACCTCGTGCGCTAAGCAAGATCGGAAGAGCACA280920.1558605843384858No Hit
GACTTGGGCCAACGTCTATCTGAAGATCGGAAGAGCACACGTCTGAACTC267740.14854803093687236Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTGAACTC267610.14847590408237996Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAACT259440.14394300868851184Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTGATGGCCGCATCGGCGACTCAGGAGATCGGAAGAGCACACGTCTGAA259320.1438764300535958Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTTGGGCCAACGTCTATCTGAGGAGATCGGAAGAGCACACGTCTGAAC257520.14287775052985496Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACAATGAGTCGATCAACGTGCGTATAGATCGGAAGAGCACACGTCTGAAC254030.14094142189771303Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTTAGCGTCATACCGCGTAGAATAAAAGATCGGAAGAGCACACGTCTGA248250.1377345509825897Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTGTTAGTATAGTGGTGAGTATTCCAGATCGGAAGAGCACACGTCTG247690.13742385068631477Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTG247580.13736282027097504Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGCGATGGATCATTCAAGTGTCTGACCAGATCGGAAGAGCACACGTCTG246930.13700218599851308Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGTGACATCCGGCGCGCTTGTGGCAGATCGGAAGAGCACACGTCTGAAC244400.13559848644569958Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGAA241160.13380086330296606Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGAATCCAGAGACGTTGGCACAAGAGATCGGAAGAGCACACGTCTGAA240980.133700995350592Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGA235640.13073824609682752Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTGAA234200.12993930247783486Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGCGACCGGGGTTCGATTCCCCGACACGGAGATCGGAAGAGCACACGTC231570.12848012072925796No Hit
GGAAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGA227220.12606664521355096Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTGTTAGTATAGTGGTGAGTATTCCCAGATCGGAAGAGCACACGTCT222070.12320931213173691Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGAGCACACGTCT216730.12024656287797247Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTAGAACATCGAAACTCTGAACGAGATCGGAAGAGCACACGTCTGAACTC211720.11746690487022715Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTTAGCGTCATACCGCGTAGAATAGATCGGAAGAGCACACGTCTGAACT209640.11631287519834885Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAA208640.11575805324071506Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCCGAGTGTCGTAAACGGTAGAGCAATTTAGATCGGAAGAGCACACGTC202090.11212396941821369No Hit
TTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTG200420.11119741674896526Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACAAGTACCGTGAGGGAAAGTTGAGATCGGAAGAGCACACGTCTGAAC200060.1109976808442171Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGCTGGTAGAGATAAATCGATGCAGATCGGAAGAGCACACGTCTGAACT192990.10707508960374615Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTGA192190.10663123203763912Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGAGAATTAGTGTGAATTGCAGGAGATCGGAAGAGCACACGTCTGAACTC190600.10574906512500139Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGAGTGTTATAGTCCAGCCGAGATCGGAAGAGCACACGTCTGAACTC190540.10571577580754335Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTATACACTGACGAGTAGGAGAGATCGGAAGAGCACACGTCTGAACTCC189350.10505553767795914Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGTCCGATCACTGAAGTCAAACTACAGATCGGAAGAGCACACGTCTGAAC187840.1042177565219321Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)