FastQCFastQC Report
Thu 28 Sept 2023
SRR3199174.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3199174.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18054928
Total Bases920.8 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGCGTCATACCGCGTAGAATAAACCAGATCGGAAGAGCACACGTCT1183670.6555938633485551Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGACCTCAGATTAGTCGAGAGCACTCGCTGAGATCGGAAGAGCACACGTC817510.45279050683558525No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAAGATCGGAAGAGCACACGTC771210.4271465386070773No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG671740.37205354682112274TruSeq Adapter, Index 10 (100% over 49bp)
GATAGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCT645340.3574315001422326Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGATAGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTC583370.3231084610251561No Hit
GACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGT568000.3145955497579387No Hit
AAAGGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGAGCACA558850.30952768130673247No Hit
AAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGAAC527010.29189260682734375Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTTAGCGTCATACCGCGTAGAATAAACAGATCGGAAGAGCACACGTCTG511140.28310276285787456Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGTGATGGCCGCATCGGCGACTCAGGAGATCGGAAGAGCACACGTCTGA416670.23077909809443714Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGAGATCGGAAGAGCACACGT398150.22052151080303395No Hit
GACTTGGGCCAACGTCTATCTGAGGGTAGATCGGAAGAGCACACGTCTGA389070.21549241293014298Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTGCGATCCGTTGAAACTCATCTCAGCGAGATCGGAAGAGCACACGTCT364750.20202240629261994Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTTGGGCCAACGTCTATCTGAGAGATCGGAAGAGCACACGTCTGAACT356080.19722039323557533Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCTGGTAGAGATAAATCGATGCAGATCGGAAGAGCACACGTCTGAACTC351320.1945839939101391Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCCGAGTGTCGTAAACGGTAGAGCAGATCGGAAGAGCACACGTCTGAAC329000.18222171808162294Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGTGTTAGTATAGTGGTGAGTATTCCCGAGATCGGAAGAGCACACGTC317840.17604058016736482No Hit
GGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGAGCACACGT312700.17319371198821729No Hit
ATTGCGATCCGTTGAAACTCATCTCAGCAGATCGGAAGAGCACACGTCTG306290.1696434347453504Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGTGACATCCGGCGCGCTTGTGGCGAGATCGGAAGAGCACACGTCTGAA297700.16488573092066608Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTTGGGCCAACGTCTATCTGAGGGTCAGATCGGAAGAGCACACGTCTG289180.160166797674297Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTACAATGAGTCGATCAACGTGCGAGATCGGAAGAGCACACGTCTGAAC286950.1589316778222544Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTACAATGAGTCGATCAACGTGCGTATAGATCGGAAGAGCACACGTCTG285880.15833904183943573Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTGACCGGTTGGGAATACCTCGTGCGCTAAGCAAGATCGGAAGAGCACA285140.15792918144010323No Hit
GACTTGGGCCAACGTCTATCTGAAGATCGGAAGAGCACACGTCTGAACTC266930.14784329242409608Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTGAACTC265830.14723404047914232Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTGATGGCCGCATCGGCGACTCAGGAGATCGGAAGAGCACACGTCTGAA260530.14429855383527423Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAACT258000.1428972743618806Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACTTGGGCCAACGTCTATCTGAGGAGATCGGAAGAGCACACGTCTGAAC257750.14275880801075475Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACAATGAGTCGATCAACGTGCGTATAGATCGGAAGAGCACACGTCTGAAC251220.13914206691934744Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTTAGCGTCATACCGCGTAGAATAAAAGATCGGAAGAGCACACGTCTGA249730.13831680746663738Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTGTTAGTATAGTGGTGAGTATTCCAGATCGGAAGAGCACACGTCTG249490.13818387976955654Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTG248000.13735862031684648Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGCGATGGATCATTCAAGTGTCTGACCAGATCGGAAGAGCACACGTCTG246910.13675490702593773Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGTGACATCCGGCGCGCTTGTGGCAGATCGGAAGAGCACACGTCTGAAC244900.1356416375628859Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGAATCCAGAGACGTTGGCACAAGAGATCGGAAGAGCACACGTCTGAA241710.13387480692252Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGAA240250.13306616343194502Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGA234650.12996451716672588Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTGAA233580.12937188118390724Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGCGACCGGGGTTCGATTCCCCGACACGGAGATCGGAAGAGCACACGTC232150.12857985365546737No Hit
GGAAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGA229780.12726719264679426Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTGTTAGTATAGTGGTGAGTATTCCCAGATCGGAAGAGCACACGTCT226120.12524004526631177Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGAGCACACGTCT217930.12070388760342882Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAA213070.11801210173754224Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTTAGCGTCATACCGCGTAGAATAGATCGGAAGAGCACACGTCTGAACT209890.11625080975122139Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTAGAACATCGAAACTCTGAACGAGATCGGAAGAGCACACGTCTGAACTC209760.11617880724863594Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCCGAGTGTCGTAAACGGTAGAGCAATTTAGATCGGAAGAGCACACGTC203260.11257868211936375No Hit
TTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTG202620.11222420826048156Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACAAGTACCGTGAGGGAAAGTTGAGATCGGAAGAGCACACGTCTGAAC202050.11190850497991463Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTGA194880.10793729002962515Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGCTGGTAGAGATAAATCGATGCAGATCGGAAGAGCACACGTCTGAACT192550.1066467836371322Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGAGTGTTATAGTCCAGCCGAGATCGGAAGAGCACACGTCTGAACTC191000.1057882922601519Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTATACACTGACGAGTAGGAGAGATCGGAAGAGCACACGTCTGAACTCC190570.10555013013621543Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGAGAATTAGTGTGAATTGCAGGAGATCGGAAGAGCACACGTCTGAACTC188040.1041488506628218Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGTCCGATCACTGAAGTCAAACTACAGATCGGAAGAGCACACGTCTGAAC187700.10396053642529066Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)