FastQCFastQC Report
Thu 28 Sept 2023
SRR3199175.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3199175.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13194548
Total Bases672.9 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGCGTCATACCGCGTAGAATAAACCAGATCGGAAGAGCACACGTCT825060.6253037239320361Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGACCTCAGATTAGTCGAGAGCACTCGCTGAGATCGGAAGAGCACACGTC605560.4589471348317502No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAAGATCGGAAGAGCACACGTC529890.4015976901974967No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG472370.35800392707654705TruSeq Adapter, Index 10 (100% over 49bp)
GATAGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCT438610.3324176015730133Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGATAGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTC421790.31966991214856316No Hit
AAAGGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGAGCACA413850.31365227516698563No Hit
AAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGAAC398580.30207931336488375Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGT381190.28889962733092484No Hit
GCTTAGCGTCATACCGCGTAGAATAAACAGATCGGAAGAGCACACGTCTG354690.26881557443271265Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGTGATGGCCGCATCGGCGACTCAGGAGATCGGAAGAGCACACGTCTGA284570.21567241257525455Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGAGATCGGAAGAGCACACGT275330.20866952016848173No Hit
ATTGCGATCCGTTGAAACTCATCTCAGCGAGATCGGAAGAGCACACGTCT270530.20503165398314516Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTGGTAGAGATAAATCGATGCAGATCGGAAGAGCACACGTCTGAACTC262430.19889275479538973Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GACTTGGGCCAACGTCTATCTGAGGGTAGATCGGAAGAGCACACGTCTGA252450.19132902468504415Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTTGGGCCAACGTCTATCTGAGAGATCGGAAGAGCACACGTCTGAACT244780.1855160176763918Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGTGTTAGTATAGTGGTGAGTATTCCCGAGATCGGAAGAGCACACGTC228760.17337463928283106No Hit
ATTGCGATCCGTTGAAACTCATCTCAGCAGATCGGAAGAGCACACGTCTG223770.16959277422765828Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGAGCACACGT220550.16715237232832833No Hit
ATCCGAGTGTCGTAAACGGTAGAGCAGATCGGAAGAGCACACGTCTGAAC217290.16468165487745393Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGTGACATCCGGCGCGCTTGTGGCGAGATCGGAAGAGCACACGTCTGAA213140.16153641640471506Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTACAATGAGTCGATCAACGTGCGTATAGATCGGAAGAGCACACGTCTG202310.15332848082404946Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTACAATGAGTCGATCAACGTGCGAGATCGGAAGAGCACACGTCTGAAC200770.1521613320895873Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTGAACTC198400.15036513566057738Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GACTTGGGCCAACGTCTATCTGAGGGTCAGATCGGAAGAGCACACGTCTG188100.14255888113787604Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACAATGAGTCGATCAACGTGCGTATAGATCGGAAGAGCACACGTCTGAAC187970.1424603555953565Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGATGGCCGCATCGGCGACTCAGGAGATCGGAAGAGCACACGTCTGAA185530.14061110695114376Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTGACCGGTTGGGAATACCTCGTGCGCTAAGCAAGATCGGAAGAGCACA185410.14052016029651035No Hit
GACTTGGGCCAACGTCTATCTGAAGATCGGAAGAGCACACGTCTGAACTC182510.13832228280953618Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAACT178250.13509367657005Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGCGATGGATCATTCAAGTGTCTGACCAGATCGGAAGAGCACACGTCTG178120.13499515102753046Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTG176930.13409326336908245Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTTGGGCCAACGTCTATCTGAGGAGATCGGAAGAGCACACGTCTGAAC176760.13396442227501845Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGAA176500.1337673711899794Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGAATCCAGAGACGTTGGCACAAGAGATCGGAAGAGCACACGTCTGAA173560.13153917815146074Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTTAGCGTCATACCGCGTAGAATAAAAGATCGGAAGAGCACACGTCTGA171650.13009161056521223Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTGAA169340.12834088746351902Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGTGACATCCGGCGCGCTTGTGGCAGATCGGAAGAGCACACGTCTGAAC168720.12787099641457972Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGTGTTAGTATAGTGGTGAGTATTCCAGATCGGAAGAGCACACGTCTG168660.127825523087263Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGA164640.12477881015704365Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGA163480.12389965916225398Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCGACCGGGGTTCGATTCCCCGACACGGAGATCGGAAGAGCACACGTC160210.12142136282349346No Hit
GAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGAGCACACGTCT154270.11691950341913948Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGTGTTAGTATAGTGGTGAGTATTCCCAGATCGGAAGAGCACACGTCT150670.11419110378013708Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACAAGTACCGTGAGGGAAAGTTGAGATCGGAAGAGCACACGTCTGAAC148950.11288753506372481Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTTAGCGTCATACCGCGTAGAATAGATCGGAAGAGCACACGTCTGAACT148220.11233427624803821Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTGA146880.11131870527129842Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTCCGATCACTGAAGTCAAACTACAGATCGGAAGAGCACACGTCTGAAC146230.11082607755870076Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTG143310.10861304229595435Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAA139030.10536927828069594Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTAGAACATCGAAACTCTGAACGAGATCGGAAGAGCACACGTCTGAACTC138470.10494486055907333Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTATGCTTGAATAAGTTGAAGCAGATCGGAAGAGCACACGTCTGAACTC133760.10137520436471184Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCCGAGTGTCGTAAACGGTAGAGCAATTTAGATCGGAAGAGCACACGTC133090.10086741887634196No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)