FastQCFastQC Report
Thu 28 Sept 2023
SRR3199176.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3199176.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13182117
Total Bases672.2 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGCGTCATACCGCGTAGAATAAACCAGATCGGAAGAGCACACGTCT848240.6434778268164363Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGACCTCAGATTAGTCGAGAGCACTCGCTGAGATCGGAAGAGCACACGTC607950.4611929935077954No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAAGATCGGAAGAGCACACGTC545650.4139319959002033No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG463280.3514458261901332TruSeq Adapter, Index 10 (100% over 49bp)
GATAGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCT459830.3488286441396325Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGATAGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTC425720.32295267899685615No Hit
AAAGGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGAGCACA411320.3120287886991141No Hit
AAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGAAC396880.30107455426165614Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GACTTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGT391040.29664430986312745No Hit
GCTTAGCGTCATACCGCGTAGAATAAACAGATCGGAAGAGCACACGTCTG368010.2791736714216692Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGTGATGGCCGCATCGGCGACTCAGGAGATCGGAAGAGCACACGTCTGA296130.22464525235210703Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGAGATCGGAAGAGCACACGT284220.2156102847516829No Hit
ATTGCGATCCGTTGAAACTCATCTCAGCGAGATCGGAAGAGCACACGTCT270210.20498224981617139Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTGGTAGAGATAAATCGATGCAGATCGGAAGAGCACACGTCTGAACTC263500.1998920203788208Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GACTTGGGCCAACGTCTATCTGAGGGTAGATCGGAAGAGCACACGTCTGA261870.1986554966853958Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTTGGGCCAACGTCTATCTGAGAGATCGGAAGAGCACACGTCTGAACT253210.19208599043689265Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGTGTTAGTATAGTGGTGAGTATTCCCGAGATCGGAAGAGCACACGTC229960.1744484592269967No Hit
ATTGCGATCCGTTGAAACTCATCTCAGCAGATCGGAAGAGCACACGTCTG229890.17439535698249378Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGAGCACACGT228370.1732422796732877No Hit
TTGTGACATCCGGCGCGCTTGTGGCGAGATCGGAAGAGCACACGTCTGAA214960.16306940683351545Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCCGAGTGTCGTAAACGGTAGAGCAGATCGGAAGAGCACACGTCTGAAC214130.16243976593440948Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTACAATGAGTCGATCAACGTGCGAGATCGGAAGAGCACACGTCTGAAC208620.15825986068853734Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTACAATGAGTCGATCAACGTGCGTATAGATCGGAAGAGCACACGTCTG208070.15784262876744304Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTTGGGCCAACGTCTATCTGAGGGTCAGATCGGAAGAGCACACGTCTG197650.14993798037143805Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTGAACTC197490.14981660381257425Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGTGACCGGTTGGGAATACCTCGTGCGCTAAGCAAGATCGGAAGAGCACA191760.14546980579826443No Hit
ACAATGAGTCGATCAACGTGCGTATAGATCGGAAGAGCACACGTCTGAAC188690.14314089307506528Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGATGGCCGCATCGGCGACTCAGGAGATCGGAAGAGCACACGTCTGAA187380.1421471224993679Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTTGGGCCAACGTCTATCTGAAGATCGGAAGAGCACACGTCTGAACTC184030.1396058007981571Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGAA181550.13772446413576817Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTTGGGCCAACGTCTATCTGAGGAGATCGGAAGAGCACACGTCTGAAC180800.13715551151609412Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAACT180690.13707206513187525Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGCGATGGATCATTCAAGTGTCTGACCAGATCGGAAGAGCACACGTCTG179660.13629070353418954Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTG179080.13585071350830827Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTAGCGTCATACCGCGTAGAATAAAAGATCGGAAGAGCACACGTCTGA178790.13563071849536762Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGAATCCAGAGACGTTGGCACAAGAGATCGGAAGAGCACACGTCTGAA174110.13208045414860148Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTGAA171030.12974395539047331Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGTGACATCCGGCGCGCTTGTGGCAGATCGGAAGAGCACACGTCTGAAC170760.12953913244739065Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGTGTTAGTATAGTGGTGAGTATTCCAGATCGGAAGAGCACACGTCTG170080.12902328207221953Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAAAAGCTCAAAGCCGAATCGTTTTGAGATCGGAAGAGCACACGTCTGA169060.1282495065094628Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGA169020.12821916236974681Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCGACCGGGGTTCGATTCCCCGACACGGAGATCGGAAGAGCACACGTC168850.12809019977595404No Hit
GAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGAGCACACGTCT160790.1219758556231901Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGTGTTAGTATAGTGGTGAGTATTCCCAGATCGGAAGAGCACACGTCT155840.1182207683333413Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTAGCGTCATACCGCGTAGAATAGATCGGAAGAGCACACGTCTGAACT152080.115368419200042Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAACAAGTACCGTGAGGGAAAGTTGAGATCGGAAGAGCACACGTCTGAAC148320.11251607006674269Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTCCGATCACTGAAGTCAAACTACAGATCGGAAGAGCACACGTCTGAAC147770.11209883814564839Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCTGCGCATAACCGCCCGATGCGAGAAGATCGGAAGAGCACACGTCTGA147120.1116057458752642Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTGAA143440.10881408502139678Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGGCCAACGTCTATCTGAGGGTCACTAGATCGGAAGAGCACACGTCTG142100.10779755634091245Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTAGAACATCGAAACTCTGAACGAGATCGGAAGAGCACACGTCTGAACTC138460.10503623962676101Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGCTGGTAGAGATAAATCGATGCAGATCGGAAGAGCACACGTCTGAACT137840.10456590546116379Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCCGAGTGTCGTAAACGGTAGAGCAATTTAGATCGGAAGAGCACACGTC134490.10202458375995298No Hit
ACTATGCTTGAATAAGTTGAAGCAGATCGGAAGAGCACACGTCTGAACTC134150.1017666585723674Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGAGAATTAGTGTGAATTGCAGGAGATCGGAAGAGCACACGTCTGAACTC132600.10059082315837434Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)